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[174918_at]

Description CEK011FXR_rc /REP_DB=TREMBL Accession /GB=D32353 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11f10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 50      4grp_high_8_8 > 32      6grp_highest_nonorm > 37      6grp_total > 39      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3310
1 2 3 4 range
A 6497.7
(3)
P 		3780.7
(-3)
P 		4484
(-1.4)
P 		4301.5
(-1.8)
P 		2717
B 6959.4
(3)
P 		4660.1
(-1.4)
P 		4041.8
(-2.5)
P 		3793.4
(-3)
P 		3166
C 5867
(3)
P 		3994.5
(-2.1)
P 		3834
(-2.5)
P 		3649.1
(-3)
P 		2218
avr 6441.4
(3) 		4145.1
(-2.5) 		4119.9
(-2.5) 		3914.7
(-3) 		2527

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2116
1 2 3 4 5 6 range
A 5936.1
(0.5)
P 		6063.1
(0.8)
P 		4791.7
(-2.9)
P 		4746.9
(-3)
P 		6813.3
(3)
P 		6295.9
(1.5)
P 		2066
B 5417.6
(-0.6)
P 		6191.5
(3)
P 		4902.5
(-3)
P 		5477.6
(-0.3)
P 		4937.1
(-2.8)
P 		6159.2
(2.8)
P 		1289
C 5812.7
(3)
P 		5820.3
(3)
P 		5799.9
(2.9)
P 		5401.4
(0.8)
P 		5351.3
(0.5)
P 		4697.2
(-3)
P 		1123
avr 5722.1
(0.9) 		6025
(3) 		5164.7
(-3) 		5208.6
(-2.7) 		5700.6
(0.7) 		5717.4
(0.9) 		860
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1751
1 2 3 4 range
A 2365.8
(3)
P 		820.7
(-2.3)
P 		1172.2
(-1.1)
P 		629.7
(-3)
P 		1736
B 2219.3
(3)
P 		1150.9
(-1.5)
P 		936.6
(-2.5)
P 		808.4
(-3)
P 		1411
C 1942.1
(3)
P 		1148.5
(-0.6)
P 		1336.3
(0.3)
P 		615.3
(-3)
P 		1327
avr 2175.7
(3) 		1040
(-1.6) 		1148.4
(-1.1) 		684.5
(-3) 		1491

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1253
1 2 3 4 5 6 range
A 1094.3
(-2.7)
P 		2156.3
(3)
P 		1043.5
(-3)
P 		1203.9
(-2.1)
P 		2055.9
(2.5)
P 		1532.8
(-0.4)
P 		1113
B 1885
(0.8)
P 		2060.9
(1.7)
P 		1190.5
(-3)
P 		1450.8
(-1.6)
P 		2005.9
(1.4)
P 		2296.7
(3)
P 		1106
C 2154.5
(3)
P 		1775.9
(0.9)
P 		1476.2
(-0.8)
P 		1086.2
(-3)
P 		1731.6
(0.6)
P 		1572.4
(-0.3)
P 		1068
avr 1711.3
(0.7) 		1997.7
(3) 		1236.7
(-3) 		1247
(-2.9) 		1931.1
(2.5) 		1800.6
(1.4) 		761
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174918_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20986
Sequence Type Exemplar sequence
Representative Public ID CEK011FXR NCBI
Target Description CEK011FXR_rc /REP_DB=TREMBL Accession /GB=D32353 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11f10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066339(11), NM_171886(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066339 NCBI Caenorhabditis elegans CaLPain family member (clp-1) (clp-1) mRNA, complete cds. 11/11 None
NM_171886 NCBI Caenorhabditis elegans CaLPain family member (clp-1) (clp-1) mRNA, complete cds. 11/11 None
C06G4.2b.1 ENSEMBL cdna:known chromosome:CEL140:III:7980998:7986252:1 gene:C06G4.2 11/11 None
C06G4.2d ENSEMBL cdna:known chromosome:CEL140:III:7981093:7986238:1 gene:C06G4.2 11/11 None
C06G4.2b.3 ENSEMBL cdna:known chromosome:CEL140:III:7981093:7986238:1 gene:C06G4.2 9/11 None
C06G4.2b.2 ENSEMBL cdna:known chromosome:CEL140:III:7981093:7986558:1 gene:C06G4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176122 4 2 NM_066339 174918_at (11), 188726_at (10)
NM_066340 188726_at (10)
NM_171886 174918_at (11), 188726_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7985759-7986229(+) 100.0 100.0
Public Domain and Genome References
Ensembl C06G4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV58888 Calpain family protein 1, isoform b [Caenorhabditis elegans] ref|NP_498740.2| CaLPain family member (clp-1) [Caenorhabditis elegans] 0.0
blast AAM15551 Calpain family protein 1, isoform d [Caenorhabditis elegans] ref|NP_741237.1| CaLPain family member (clp-1) [Caenorhabditis elegans] 0.0
blast AAV58887 Calpain family protein 1, isoform a [Caenorhabditis elegans] ref|NP_498741.3| CaLPain family member (clp-1) [Caenorhabditis elegans] sp|P34308|CAN_CAEEL Calpain clp-1 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.0E-126
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 5.1E-101
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.0E-126
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.5E-97
Sequence
Target Sequence 
>C. ELEGANS:174918_AT
gagtcggaggaactgtaagccattctactcacaactacaacaacaaatagcaagattgga
tcttcctatacctattgttttgatccacttcgttatgtataaatctctctgtcactcgtt
ctattttcatcattaacccccctaccctctctctctttcctcaatcgtccctcttttctc
cactctttctgcctaatttcccgtcgatgtttgccggatctgctctgtagaattgtaata
ttttgatatgcttgttgtttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCGGAGGAACTGTAAGCCATTC 291 399 87 Antisense
GTAAGCCATTCTACTCACAACTACA 291 463 101 Antisense
CATTCTACTCACAACTACAACAACA 612 215 107 Antisense
GATTGGATCTTCCTATACCTATTGT 289 435 140 Antisense
TACCTATTGTTTTGATCCACTTCGT 488 649 155 Antisense
GTTTTGATCCACTTCGTTATGTATA 34 449 163 Antisense
ATGTATAAATCTCTCTGTCACTCGT 306 47 181 Antisense
GTCACTCGTTCTATTTTCATCATTA 350 465 197 Antisense
ATTTCCCGTCGATGTTTGCCGGATC 309 15 282 Antisense
TTTGCCGGATCTGCTCTGTAGAATT 665 667 296 Antisense
TATTTTGATATGCTTGTTGTTTCAC 384 663 325 Antisense
Sequence Source Affymetrix Proprietary Database