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[174913_s_at]

Description CEK013FXR_rc /REP_DB=TREMBL Accession /GB=D32398 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13f10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 29      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 50      6grp_total > 29      4grp_total > 10      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 671
1 2 3 4 range
A 301.4
(-1.5)
P 		187
(-3)
P 		351.8
(-0.8)
P 		640.6
(3)
P 		454
B 314.5
(-1.9)
P 		548.6
(0.3)
P 		202.3
(-3)
P 		833.3
(3)
P 		631
C 216.4
(-3)
P 		307.9
(-2.1)
P 		226.9
(-2.9)
P 		858
(3)
P 		642
avr 277.4
(-2.8) 		347.8
(-2) 		260.3
(-3) 		777.3
(3) 		517

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 616
1 2 3 4 5 6 range
A 627.4
(1.1)
P 		264.5
(-2.6)
P 		417.3
(-1)
P 		222.3
(-3)
P 		819.7
(3)
P 		635.6
(1.2)
P 		597
B 564.8
(0.3)
P 		837.9
(3)
P 		231.3
(-3)
P 		539.2
(0)
P 		597
(0.6)
P 		443.2
(-0.9)
P 		607
C 825.3
(3)
P 		758.5
(2.2)
P 		592.9
(0.3)
P 		316.6
(-3)
P 		545.8
(-0.3)
P 		466.9
(-1.2)
P 		509
avr 672.5
(3) 		620.3
(2) 		413.8
(-2) 		359.4
(-3) 		654.2
(2.6) 		515.2
(-0) 		313
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 109.7
(3)
P 		40.6
(-3)
P 		92
(1.5)
P 		93.8
(1.6)
P 		69
B 100.3
(-0.5)
P 		135.5
(1.1)
P 		46.9
(-3)
P 		177.6
(3)
P 		131
C 71.6
(-3)
P 		88.5
(-1.6)
P 		79.1
(-2.4)
P 		144.7
(3)
P 		73
avr 93.9
(-1.1) 		88.2
(-1.6) 		72.7
(-3) 		138.7
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 115.7
(-1.1)
P 		94.1
(-1.8)
P 		90.9
(-1.9)
P 		56.4
(-3)
P 		247.3
(3)
P 		154.8
(0.1)
P 		191
B 196.5
(0.8)
P 		278.9
(3)
P 		56.2
(-3)
P 		142.8
(-0.7)
P 		242.6
(2)
P 		165.3
(-0.1)
P 		223
C 305.9
(3)
P 		231.4
(1.2)
P 		150.9
(-0.8)
P 		63.7
(-3)
P 		176.6
(-0.2)
P 		156.3
(-0.7)
P 		242
avr 206
(2.3) 		201.5
(2.1) 		99.3
(-2.5) 		87.6
(-3) 		222.2
(3) 		158.8
(0.2) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174913_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21457
Sequence Type Exemplar sequence
Representative Public ID CEK013FXR NCBI
Target Description CEK013FXR_rc /REP_DB=TREMBL Accession /GB=D32398 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13f10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068719(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068719 NCBI Caenorhabditis elegans C34D4.14 (C34D4.14) mRNA, complete cds. 11/11 None
C34D4.14 ENSEMBL cdna:known chromosome:CEL140:IV:7134330:7144547:-1 gene:C34D4.14 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7134358-7134704(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C34D4.14
Entrez Gene 177486 Entrez gene
SwissProt Q18447 EMBL-EBI
WormBase CE17508 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605993_S_AT similar to HECT domain containing protein 1 cfa
CANINE:1597064_AT similar to HECT domain containing protein 1 cfa
CANINE_2:CFA.2009.1.S1_S_AT similar to HECT domain containing protein 1 cfa
CANINE_2:CFAAFFX.20016.1.S1_S_AT similar to HECT domain containing protein 1 cfa
DROSOPHILA_2:1634156_AT dm
DROSGENOME1:146135_AT dm
CHICKEN:GGA.3240.1.S1_AT HECT domain containing 1 gga
CHICKEN:GGAAFFX.23079.1.S1_S_AT HECT domain containing 1 gga
HG-U133B:224481_S_AT HECT domain containing 1 hs
HG-U133_PLUS_2:224481_S_AT HECT domain containing 1 hs
HU35KSUBA:RC_AA441821_AT HECT domain containing 1 hs
HU35KSUBA:W58540_AT HECT domain containing 1 hs
U133_X3P:G13623274_3P_S_AT HECT domain containing 1 hs
U133_X3P:HS2.421481.1.S1_3P_X_AT HECT domain containing 1 hs
U133_X3P:HS.322848.0.A1_3P_AT HECT domain containing 1 hs
U133_X3P:HS.314346.0.S1_3P_AT HECT domain containing 1 hs
U133_X3P:HS.190223.0.S1_3P_AT HECT domain containing 1 hs
HG-U133_PLUS_2:1557100_S_AT HECT domain containing 1 hs
HG-U133_PLUS_2:241683_AT HECT domain containing 1 hs
HG-U133B:241683_AT HECT domain containing 1 hs
HG-U133_PLUS_2:241955_AT HECT domain containing 1 hs
HG-U133B:241955_AT HECT domain containing 1 hs
HG-U133_PLUS_2:242349_AT HECT domain containing 1 hs
HG-U133B:242349_AT HECT domain containing 1 hs
HG-U95B:45304_AT HECT domain containing 1 hs
HG-U95E:68210_AT HECT domain containing 1 hs
HG-U95D:72016_AT HECT domain containing 1 hs
HG-U95D:85047_AT HECT domain containing 1 hs
U133_X3P:HS2.407239.1.S1_3P_AT HECT domain containing 1 hs
HG-U133_PLUS_2:1570251_AT HECT domain containing 1 hs
HG-U95E:78021_AT HECT domain containing 1 hs
HG-U95D:81798_AT HECT domain containing 1 hs
HG-U95E:86985_I_AT HECT domain containing 1 hs
HG-U95E:86988_R_AT HECT domain containing 1 hs
MG-U74BV2:108507_AT HECT domain containing 1 mm
MG-U74BV2:116116_AT HECT domain containing 1 mm
MOE430A:1424141_AT HECT domain containing 1 mm
MOUSE430A_2:1424141_AT HECT domain containing 1 mm
MOUSE430_2:1424141_AT HECT domain containing 1 mm
MOE430B:1438039_AT HECT domain containing 1 mm
MOUSE430_2:1438039_AT HECT domain containing 1 mm
MU19KSUBB:TC31559_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
MU19KSUBB:TC31559_G_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
MU19KSUBC:TC36278_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
RG-U34B:RC_AI059295_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI228159_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI178224_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI175474_AT similar to HECT domain containing 1 rn
RAE230A:1372394_AT similar to HECT domain containing 1 rn
RAT230_2:1372394_AT similar to HECT domain containing 1 rn
RAE230B:1392259_AT similar to HECT domain containing 1 rn
RAT230_2:1392259_AT similar to HECT domain containing 1 rn
RAE230B:1383780_AT similar to HECT domain containing 1 rn
RAT230_2:1383780_AT similar to HECT domain containing 1 rn
RAE230B:1383615_A_AT similar to HECT domain containing 1 rn
RAT230_2:1383615_A_AT similar to HECT domain containing 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00699 Hypothetical protein C34D4.14 [Caenorhabditis elegans] ref|NP_501120.1| C34D4.14 [Caenorhabditis elegans] 0.0
blast CAE61812 Hypothetical protein CBG05779 [Caenorhabditis briggsae] 0.0
blast EAL41378 ENSANGP00000025754 [Anopheles gambiae str. PEST] ref|XP_559736.1| ENSANGP00000025754 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 0.027
Pfam IPR002110 EMBL-EBI Ankyrin 44.0
Pfam IPR002110 EMBL-EBI Ankyrin 0.0024
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 9.2E-39
Pfam IPR010606 EMBL-EBI Mib_herc2 2.7E-32
Sequence
Target Sequence 
>C. ELEGANS:174913_S_AT
ttgtgtttttctcttgtctttaactatttgttcaatacttttttcgggacttattatcca
aatcgtttgcaattttctcaattattggtagagaattattgtacggtcgtggcgccttca
tactcctctacagtattccttggccccgtaaaatctagcatttttacacgaacgtcaata
cccaacttgtttcagctattttcgctgaaatttctttttacaattacatttcaattggta
ccccttctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTGTTTTTCTCTTGTCTTTAACT 576 703 13 Antisense
TTCTCTTGTCTTTAACTATTTGTTC 572 691 21 Antisense
TTTCGGGACTTATTATCCAAATCGT 209 559 54 Antisense
TCCAAATCGTTTGCAATTTTCTCAA 185 589 69 Antisense
AATTATTGTACGGTCGTGGCGCCTT 462 173 106 Antisense
TTCATACTCCTCTACAGTATTCCTT 319 689 129 Antisense
TTCCTTGGCCCCGTAAAATCTAGCA 687 617 148 Antisense
TTACACGAACGTCAATACCCAACTT 477 657 176 Antisense
CCCAACTTGTTTCAGCTATTTTCGC 2 259 193 Antisense
TTTCGCTGAAATTTCTTTTTACAAT 322 671 212 Antisense
TACATTTCAATTGGTACCCCTTCTT 325 641 237 Antisense
Sequence Source Affymetrix Proprietary Database