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[174895_at] importin beta, nuclear transport factor

Description CEK094A9R_rc /REP_DB=TREMBL Accession /GB=D66234 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk94a9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 01      6grp_total > 27      4grp_total > 02      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3814
1 2 3 4 range
A 3712.2
(-2.6)
P 		3824.4
(-2.2)
P 		3560.8
(-3)
P 		5619.3
(3)
P 		2059
B 4053
(-2)
P 		3427.8
(-3)
P 		3579.8
(-2.8)
P 		7241.9
(3)
P 		3814
C 3786.3
(-2.7)
P 		3602.6
(-3)
P 		3878.4
(-2.5)
P 		6994.8
(3)
P 		3392
avr 3850.5
(-2.5) 		3618.3
(-3) 		3673
(-2.9) 		6618.7
(3) 		3000

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1816
1 2 3 4 5 6 range
A 2885.3
(3)
P 		2256.2
(0.9)
P 		1275.5
(-2.3)
P 		1069.5
(-3)
P 		1898.6
(-0.3)
P 		2056.6
(0.3)
P 		1816
B 2658.5
(3)
P 		2360.8
(1.6)
P 		1382.7
(-3)
P 		1633.3
(-1.8)
P 		1422.4
(-2.8)
P 		1963.3
(-0.3)
P 		1276
C 2622.1
(3)
P 		2004.2
(0.4)
P 		1565.4
(-1.4)
P 		1188.6
(-3)
P 		1795.6
(-0.5)
P 		1669.3
(-1)
P 		1434
avr 2722
(3) 		2207.1
(0.8) 		1407.9
(-2.5) 		1297.1
(-3) 		1705.5
(-1.3) 		1896.4
(-0.5) 		1425
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 721
1 2 3 4 range
A 1351.6
(3)
P 		830.2
(-2.9)
P 		930.9
(-1.8)
P 		822.6
(-3)
P 		529
B 1292.5
(0.9)
P 		846.6
(-2.9)
P 		829.5
(-3)
P 		1543.2
(3)
P 		714
C 1253.4
(1.1)
P 		1035.8
(-3)
P 		1351.7
(3)
P 		1179.4
(-0.3)
P 		316
avr 1299.2
(3) 		904.2
(-3) 		1037.4
(-1) 		1181.7
(1.2) 		395

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 733
1 2 3 4 5 6 range
A 531.9
(-0.1)
P 		802.4
(3)
P 		277.8
(-2.9)
P 		271.2
(-3)
P 		572.9
(0.4)
P 		500.7
(-0.4)
P 		531
B 925
(3)
P 		785.8
(1.6)
P 		335.8
(-3)
P 		432.6
(-2)
P 		577.9
(-0.5)
P 		732.1
(1)
P 		589
C 971.9
(3)
P 		611.5
(0)
P 		398.4
(-1.7)
P 		239
(-3)
P 		581
(-0.2)
P 		558.8
(-0.4)
P 		733
avr 809.6
(3) 		733.2
(2.1) 		337.3
(-2.7) 		314.3
(-3) 		577.3
(0.2) 		597.2
(0.4) 		495
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174895_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20435
Sequence Type Exemplar sequence
Representative Public ID CEK094A9R NCBI
Target Description CEK094A9R_rc /REP_DB=TREMBL Accession /GB=D66234 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk94a9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058314(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058314 NCBI Caenorhabditis elegans IMportin Beta family member (imb-3) (imb-3) mRNA, complete cds. 11/11 None
C53D5.6.1 ENSEMBL cdna:known chromosome:CEL140:I:310982:314990:-1 gene:C53D5.6 11/11 None
C53D5.6.2 ENSEMBL cdna:known chromosome:CEL140:I:310993:314992:-1 gene:C53D5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:311002-311350(-) 100.0 100.0
Public Domain and Genome References
Gene Title importin beta, nuclear transport factor
Gene Symbol imb-3
Ensembl C53D5.6
Entrez Gene 3565440 Entrez gene
SwissProt Q9N5V3 EMBL-EBI
WormBase CE26971 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245941_AT PBS lyase HEAT-like repeat-containing protein at
CANINE:1594347_AT similar to RAN binding protein 5 cfa
CANINE:1588870_AT similar to RAN binding protein 5 cfa
CANINE_2:CFA.10369.1.S1_AT similar to RAN binding protein 5 cfa
CANINE_2:CFA.18531.1.S1_S_AT similar to RAN binding protein 5 cfa
CANINE_2:CFAAFFX.9307.1.S1_S_AT similar to RAN binding protein 5 cfa
DROSOPHILA_2:1623739_AT Karyopherin 3 dm
DROSGENOME1:141618_AT Karyopherin 3 dm
CHICKEN:GGA.17602.1.S1_S_AT RAN binding protein 5 gga
CHICKEN:GGA.2487.1.S1_AT RAN binding protein 5 gga
CHICKEN:GGAAFFX.25111.1.S1_S_AT RAN binding protein 5 gga
HG-U133_PLUS_2:211952_AT RAN binding protein 5 hs
HG-U133A_2:211952_AT RAN binding protein 5 hs
HG-U133A:211952_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211953_S_AT RAN binding protein 5 hs
HG-U133A_2:211953_S_AT RAN binding protein 5 hs
HG-U133A:211953_S_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211955_AT RAN binding protein 5 hs
HG-U133A_2:211955_AT RAN binding protein 5 hs
HG-U133A:211955_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211954_S_AT RAN binding protein 5 hs
HG-U133A_2:211954_S_AT RAN binding protein 5 hs
HG-FOCUS:211954_S_AT RAN binding protein 5 hs
HG-U133A:211954_S_AT RAN binding protein 5 hs
HG-U95AV2:39028_AT RAN binding protein 5 hs
HU35KSUBA:RC_AA056451_AT RAN binding protein 5 hs
HU35KSUBA:RC_AA283922_AT RAN binding protein 5 hs
HUGENEFL:U72761_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S4_3P_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S3_3P_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S3_3P_A_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S2_3P_A_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S1_3P_AT RAN binding protein 5 hs
U133_X3P:211953_3P_S_AT RAN binding protein 5 hs
HG-U95B:44108_AT RAN binding protein 5 hs
HG-U95E:87848_S_AT RAN binding protein 5 hs
HG-U133_PLUS_2:236906_X_AT RAN binding protein 5 hs
HG-U133B:236906_X_AT RAN binding protein 5 hs
MOE430A:1426945_AT RAN binding protein 5 mm
MOUSE430A_2:1426945_AT RAN binding protein 5 mm
MOUSE430_2:1426945_AT RAN binding protein 5 mm
MOE430A:1426946_AT RAN binding protein 5 mm
MOUSE430_2:1426946_AT RAN binding protein 5 mm
MOUSE430A_2:1426946_AT RAN binding protein 5 mm
MOUSE430A_2:1431706_AT RAN binding protein 5 mm
MOE430A:1431706_AT RAN binding protein 5 mm
MOUSE430_2:1431706_AT RAN binding protein 5 mm
MU11KSUBA:AA409333_RC_S_AT RAN binding protein 5 mm
MU11KSUBA:AA120563_G_AT RAN binding protein 5 mm
MU11KSUBA:AA120563_AT RAN binding protein 5 mm
MG-U74AV2:93070_AT RAN binding protein 5 mm
MG-U74AV2:161757_F_AT RAN binding protein 5 mm
MU19KSUBA:TC24224_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBB:TC27232_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBB:TC27232_G_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBC:TC36343_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
RAT230_2:1396742_AT karyopherin (importin) beta 3 (predicted) rn
RAE230B:1396742_AT karyopherin (importin) beta 3 (predicted) rn
RG-U34B:RC_AA925863_AT karyopherin (importin) beta 3 (predicted) rn
RG-U34C:RC_AI137586_AT karyopherin (importin) beta 3 (predicted) rn
RAE230A:1373955_AT karyopherin (importin) beta 3 (predicted) rn
RAT230_2:1373955_AT karyopherin (importin) beta 3 (predicted) rn
RAE230B:1391470_AT Karyopherin (importin) beta 3 (predicted) rn
RAT230_2:1391470_AT Karyopherin (importin) beta 3 (predicted) rn
RG-U34C:RC_AI236903_AT Karyopherin (importin) beta 3 (predicted) rn
RG-U34B:RC_AA964226_AT Karyopherin (importin) beta 3 (predicted) rn
YEAST_2:1775694_AT Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Sc
Protein Similarities
Method ID Description E-Value
blast AAF39782 Importin beta family protein 3 [Caenorhabditis elegans] ref|NP_490715.1| IMportin Beta family member (imb-3) [Caenorhabditis elegans] 0.0
blast CAE74344 Hypothetical protein CBG22062 [Caenorhabditis briggsae] 0.0
blast XP_542647 PREDICTED: similar to RAN binding protein 5 isoform 1 [Canis familiaris] 0.0
Sequence
Target Sequence 
>C. ELEGANS:174895_AT
gaatcttgttcactgttaatcgcttgtttcgcggctatcctcctatcatcatcatcagtt
tcttcttttgttccaccacttctccccgatgccacattttctctaatttttattagttac
ctatcgactttttttctgttgtgttgaatattatcctcattttcaaatctactcgttagt
tgttacagttaaagtttttgaaaataatttcagccaaaacctccactttctcctatactc
caaatctaaaagtcattcttccgtagtcctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCTTGTTCACTGTTAATCGCTT 54 329 19 Antisense
GTTCACTGTTAATCGCTTGTTTCGC 130 445 26 Antisense
TTAATCGCTTGTTTCGCGGCTATCC 66 661 34 Antisense
TCATCAGTTTCTTCTTTTGTTCCAC 120 621 70 Antisense
CGATGCCACATTTTCTCTAATTTTT 304 249 105 Antisense
TAGTTACCTATCGACTTTTTTTCTG 613 651 132 Antisense
ATCGACTTTTTTTCTGTTGTGTTGA 289 37 141 Antisense
AAATCTACTCGTTAGTTGTTACAGT 643 117 183 Antisense
ATAATTTCAGCCAAAACCTCCACTT 376 25 222 Antisense
CTTTCTCCTATACTCCAAATCTAAA 344 223 244 Antisense
TAAAAGTCATTCTTCCGTAGTCCTC 494 639 265 Antisense
Sequence Source Affymetrix Proprietary Database