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[174892_at]

Description CEK128E9R_rc /REP_DB=TREMBL Accession /GB=D64953 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk128e9 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 27      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_total > 27      4grp_total > 14      6grp_cgc > 05      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 432
1 2 3 4 range
A 422.4
(1.3)
P 		211.7
(-2.3)
P 		167.4
(-3)
P 		524.9
(3)
P 		358
B 418.6
(0.2)
P 		208.1
(-3)
P 		252.5
(-2.3)
P 		599.6
(3)
P 		392
C 461.3
(3)
P 		257.4
(-2.5)
P 		237.8
(-3)
P 		394
(1.2)
P 		224
avr 434.1
(1.5) 		225.7
(-2.9) 		219.2
(-3) 		506.2
(3) 		287

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 307.1
(3)
P 		225.7
(0.7)
P 		157.2
(-1.3)
P 		96.7
(-3)
A 		104.8
(-2.8)
A 		223.4
(0.6)
P 		210
B 379.9
(3)
P 		284.4
(0.7)
P 		155.4
(-2.5)
P 		187.1
(-1.7)
P 		135.4
(-3)
M 		221.1
(-0.9)
P 		245
C 408.2
(3)
P 		240
(-0.1)
P 		188.2
(-1.1)
P 		84.5
(-3)
A 		145.5
(-1.9)
P 		186.9
(-1.1)
P 		324
avr 365.1
(3) 		250
(0.1) 		166.9
(-1.9) 		122.8
(-3) 		128.6
(-2.9) 		210.5
(-0.8) 		242
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 153.8
(3)
P 		46
(-2.9)
P 		43.8
(-3)
P 		76.8
(-1.2)
P 		110
B 133.5
(3)
P 		51.4
(-3)
P 		58.5
(-2.5)
P 		127.8
(2.6)
P 		82
C 152.7
(3)
P 		74
(-2.5)
P 		82.9
(-1.9)
P 		66.4
(-3)
P 		86
avr 146.7
(3) 		57.1
(-3) 		61.7
(-2.7) 		90.3
(-0.8) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 56.6
(0.5)
P 		80.3
(3)
P 		34.2
(-2)
P 		24.5
(-3)
A 		31.6
(-2.2)
A 		54.4
(0.2)
P 		56
B 132.2
(3)
P 		94.7
(0.6)
P 		37.7
(-3)
P 		49.6
(-2.2)
P 		55
(-1.9)
M 		82.4
(-0.2)
P 		95
C 151.3
(3)
P 		73.2
(-0.5)
P 		47.9
(-1.6)
P 		17
(-3)
A 		47.1
(-1.7)
P 		62.6
(-1)
P 		134
avr 113.4
(3) 		82.7
(0.8) 		39.9
(-2.3) 		30.4
(-3) 		44.6
(-2) 		66.5
(-0.4) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174892_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21443
Sequence Type Exemplar sequence
Representative Public ID CEK128E9R NCBI
Target Description CEK128E9R_rc /REP_DB=TREMBL Accession /GB=D64953 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk128e9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070273(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070273 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-66) (lin-66) mRNA, complete cds. 11/11 None
B0513.1 ENSEMBL cdna:known chromosome:CEL140:IV:13888058:13893950:1 gene:B0513.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13893046-13893372(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-66
Ensembl B0513.1
Entrez Gene 178354 Entrez gene
SwissProt O62020 EMBL-EBI
WormBase CE15557 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05113 Hypothetical protein B0513.1 [Caenorhabditis elegans] ref|NP_502674.1| abnormal cell LINeage family member (lin-66) [Caenorhabditis elegans] 0.0
blast CAE74652 Hypothetical protein CBG22449 [Caenorhabditis briggsae] 0.0
blast CAE61435 Hypothetical protein CBG05318 [Caenorhabditis briggsae] 1.0E-97
Sequence
Target Sequence 
>C. ELEGANS:174892_AT
gattttggtgcattcattcaggtattttcacactctttgatcgattcacacccacaccca
cacacaccactcccatttctgtcgcaaccctgttacgtgtaaaaatttgcaaccatccga
atatacatctattgtaaacctgtaaaaccctaccacaaccccccgtaccgtagaaatttc
aaaattagtactactttggaaagcgcgacccgggaagcgtcggctgcaacacacagagcg
ttgctgaggctatagctgtgatgcgcgcaactcggaaagtgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTTGGTGCATTCATTCAGGTAT 482 429 25 Antisense
CATTCAGGTATTTTCACACTCTTTG 484 215 39 Antisense
TCACACTCTTTGATCGATTCACACC 501 627 52 Antisense
TGTCGCAACCCTGTTACGTGTAAAA 14 557 104 Antisense
TTGCAACCATCCGAATATACATCTA 411 703 131 Antisense
GTACTACTTTGGAAAGCGCGACCCG 104 459 212 Antisense
TTTGGAAAGCGCGACCCGGGAAGCG 454 437 219 Antisense
GTCGGCTGCAACACACAGAGCGTTG 432 471 243 Antisense
AGAGCGTTGCTGAGGCTATAGCTGT 274 69 259 Antisense
GCTATAGCTGTGATGCGCGCAACTC 256 303 273 Antisense
TGATGCGCGCAACTCGGAAAGTGTC 457 565 283 Antisense
Sequence Source Affymetrix Proprietary Database