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[174873_at]

Description CEK120C8R_rc /REP_DB=TREMBL Accession /GB=D66661 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk120c8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 16      6grp_total > 23      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7992
1 2 3 4 range
A 8178.8
(2.1)
P 		8769
(2.6)
P 		9325.1
(3)
P 		1460.7
(-3)
P 		7864
B 8792.9
(2.4)
P 		9452.2
(3)
P 		8982.2
(2.6)
P 		2288.1
(-3)
P 		7164
C 8688.8
(2.5)
P 		9328
(3)
P 		8609.8
(2.4)
P 		2264.2
(-3)
P 		7064
avr 8553.5
(2.5) 		9183.1
(3) 		8972.4
(2.8) 		2004.3
(-3) 		7179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5667
1 2 3 4 5 6 range
A 9311.1
(3)
P 		8806.8
(2.2)
P 		9194.9
(2.8)
P 		6624.7
(-1.3)
P 		6539.6
(-1.4)
P 		5526.2
(-3)
P 		3785
B 8582.2
(3)
P 		8256.4
(2.5)
P 		7187.5
(0.8)
P 		7700.2
(1.6)
P 		5950.2
(-1.1)
P 		4728.1
(-3)
P 		3854
C 10395.2
(3)
P 		7269.6
(-0.4)
P 		8891.2
(1.4)
P 		7972.9
(0.3)
P 		8183.3
(0.6)
P 		4921.5
(-3)
P 		5474
avr 9429.5
(3) 		8110.9
(1.2) 		8424.5
(1.6) 		7432.6
(0.3) 		6891
(-0.5) 		5058.6
(-3) 		4371
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2787
1 2 3 4 range
A 2977.9
(3)
P 		1903.5
(0.7)
P 		2437.8
(1.8)
P 		213.8
(-3)
P 		2764
B 2804
(3)
P 		2334.5
(1.8)
P 		2081.4
(1.1)
P 		487.6
(-3)
P 		2316
C 2876.2
(2.7)
P 		2681.9
(2.3)
P 		3000.8
(3)
P 		381.8
(-3)
P 		2619
avr 2886
(3) 		2306.6
(1.6) 		2506.7
(2.1) 		361.1
(-3) 		2525

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2508
1 2 3 4 5 6 range
A 1716.4
(-1.8)
P 		3132.1
(3)
P 		2002.5
(-0.8)
P 		1680.2
(-1.9)
P 		1973.4
(-0.9)
P 		1345.5
(-3)
P 		1787
B 2986.2
(3)
P 		2748.2
(1.8)
P 		1745.3
(-3)
P 		2039.5
(-1.6)
P 		2417.5
(0.3)
P 		1763.1
(-2.9)
P 		1241
C 3853
(3)
P 		2218.2
(-1.4)
P 		2263
(-1.2)
P 		1603.4
(-3)
P 		2648
(-0.2)
P 		1647.5
(-2.9)
P 		2250
avr 2851.9
(3) 		2699.5
(2.3) 		2003.6
(-1) 		1774.4
(-2.1) 		2346.3
(0.6) 		1585.4
(-3) 		1267
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174873_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22357
Sequence Type Exemplar sequence
Representative Public ID CEK120C8R NCBI
Target Description CEK120C8R_rc /REP_DB=TREMBL Accession /GB=D66661 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk120c8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078428(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078428 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-1) (mlc-1) mRNA, complete cds. 11/11 None
C36E6.3 ENSEMBL cdna:known chromosome:CEL140:X:17459085:17460757:1 gene:C36E6.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17460337-17460673(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mlc-1
Ensembl C36E6.3
Entrez Gene 181776 Entrez gene
WormBase CE34269 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21284.1.S1_AT similar to myosin light chain 2, precursor lymphocyte-specific cfa
CHICKEN:GGA.13194.1.S1_AT similar to myosin L2B regulatory light chain, cardiac muscle - chicken gga
HG-U133_PLUS_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
U133_X3P:G12803868_3P_A_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133_PLUS_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
MOE430A:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430A_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MU11KSUBB:X65979_S_AT myosin light chain 2, precursor lymphocyte-specific mm
MG-U74AV2:101195_AT myosin light chain 2, precursor lymphocyte-specific mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5859 muscle myosin inferred from direct assay QuickGO AmiGO
5859 muscle myosin inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK09070 Myosin light chain protein 1 [Caenorhabditis elegans] ref|NP_510829.2| Myosin Light Chain family member (mlc-1) [Caenorhabditis elegans] sp|P19625|MLR1_CAEEL Myosin regulatory light chain 1 gb|AAA28113.1| myosin light chain 2.0E-81
blast AAK09069 Myosin light chain protein 2 [Caenorhabditis elegans] ref|NP_510828.1| Myosin Light Chain family member (mlc-2) [Caenorhabditis elegans] sp|P19626|MLR2_CAEEL Myosin regulatory light chain 2 gb|AAA28114.1| myosin light chain 2.0E-81
blast CAE58685 Hypothetical protein CBG01859 [Caenorhabditis briggsae] emb|CAE58684.1| Hypothetical protein CBG01858 [Caenorhabditis briggsae] 1.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Sequence
Target Sequence 
>C. ELEGANS:174873_AT
aataacgtcatttcgaagccatctggcaaccccaatttcctaaccacgccgtctgactgc
gcgtacacacttctatcactccaaatggtttttccttcttttttctcgctcgcaaattct
attgtcatttcattacatgttttgtcattttttttttgtgttcacccactttcattactt
tggtattccaaaacatttgcaaacttcttctagattttctctgagcagttacttgtggtg
catatacattctcccatatcgggactagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATAACGTCATTTCGAAGCCATCTG 637 171 44 Antisense
ATTTCGAAGCCATCTGGCAACCCCA 157 13 53 Antisense
TACACACTTCTATCACTCCAAATGG 539 641 107 Antisense
TTTTTCTCGCTCGCAAATTCTATTG 318 675 143 Antisense
TTTTTTTGTGTTCACCCACTTTCAT 682 613 194 Antisense
GTTCACCCACTTTCATTACTTTGGT 258 445 203 Antisense
AAAACATTTGCAAACTTCTTCTAGA 289 129 233 Antisense
ACTTCTTCTAGATTTTCTCTGAGCA 124 103 246 Antisense
TCTCTGAGCAGTTACTTGTGGTGCA 125 609 261 Antisense
TTGTGGTGCATATACATTCTCCCAT 243 707 276 Antisense
ACATTCTCCCATATCGGGACTAGTC 33 105 289 Antisense
Sequence Source Affymetrix Proprietary Database