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[174864_at]

Description CEK085E9R_rc /REP_DB=TREMBL Accession /GB=D72021 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk85e9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 39      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 0      6grp_total > 39      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1417
1 2 3 4 range
A 2352.3
(3)
P
1594.2
(-2.4)
P
1510.3
(-3)
P
2148.8
(1.5)
P
842
B 2725.9
(2.9)
P
1516.7
(-2.2)
P
1338.1
(-3)
P
2754.7
(3)
P
1417
C 1911.1
(1.7)
P
1621.4
(-2.3)
P
1568.7
(-3)
P
2007.3
(3)
P
439
avr 2329.8
(3)
1577.4
(-2.3)
1472.4
(-3)
2303.6
(2.8)
857

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2223
1 2 3 4 5 6 range
A 2139.8
(0.9)
P
1516.6
(-1.7)
P
1207.8
(-3)
P
1444.9
(-2)
P
1758
(-0.7)
P
2651.9
(3)
P
1444
B 2310.7
(3)
P
1776.1
(0.3)
P
1104.9
(-3)
P
1610.4
(-0.5)
P
1457.4
(-1.2)
P
2042.4
(1.7)
P
1206
C 3328.1
(3)
P
1565.5
(-2.1)
P
1247.5
(-3)
P
1433.5
(-2.5)
P
1827.2
(-1.3)
P
1559.9
(-2.1)
P
2081
avr 2592.9
(3)
1619.4
(-1.2)
1186.7
(-3)
1496.3
(-1.7)
1680.9
(-0.9)
2084.7
(0.8)
1406
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 559
1 2 3 4 range
A 856.5
(3)
P
346.1
(-2.7)
P
394.8
(-2.1)
P
314.5
(-3)
P
542
B 869.3
(3)
P
374.6
(-2.3)
P
310.1
(-3)
P
587
(-0)
P
559
C 632.6
(3)
P
466.2
(-0.4)
P
546.7
(1.2)
P
338.5
(-3)
P
294
avr 786.1
(3)
395.6
(-3)
417.2
(-2.7)
413.3
(-2.7)
391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 971
1 2 3 4 5 6 range
A 394.5
(-0.9)
P
539.4
(1.3)
P
263
(-3)
P
366.5
(-1.4)
P
530.5
(1.2)
P
645.7
(3)
P
383
B 804
(3)
P
591.2
(0.6)
P
268.3
(-3)
P
426.5
(-1.2)
P
592.1
(0.6)
P
761.6
(2.5)
P
536
C 1233.6
(3)
P
477.7
(-1.8)
P
317.5
(-2.8)
P
288.3
(-3)
P
591.2
(-1.1)
P
522.2
(-1.5)
P
945
avr 810.7
(3)
536.1
(-0.1)
282.9
(-3)
360.4
(-2.1)
571.3
(0.3)
643.2
(1.1)
528
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174864_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20065
Sequence Type Exemplar sequence
Representative Public ID CEK085E9R NCBI
Target Description CEK085E9R_rc /REP_DB=TREMBL Accession /GB=D72021 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk85e9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058712
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_058712 NCBI Caenorhabditis elegans Vasa- and Belle-like Helicase family member (vbh-1) (vbh-1) mRNA, complete cds. None
Y54E10A.9a ENSEMBL cdna:known chromosome:CEL140:I:3158515:3162372:-1 gene:Y54E10A.9 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3158554-3158854(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y54E10A.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60764 Vasa- and belle-like helicase protein 1, isoform a [Caenorhabditis elegans] ref|NP_491113.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
blast AAK68520 Vasa- and belle-like helicase protein 1, isoform b [Caenorhabditis elegans] ref|NP_491112.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
blast AAU20831 Vasa- and belle-like helicase protein 1, isoform c [Caenorhabditis elegans] ref|NP_001021793.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Sequence
Target Sequence
>C. ELEGANS:174864_AT
accatttcataatactcaagttgcactatcatatgcgtctcgtcaatagctaattccaaa
cattttctaggccggtctaagccgttgaacgtgcagtccgtttccacgctttctcttcaa
tttctatttgccaatttctccccaaccaactgcattcttgtccaatatgcacaatttttc
ttttttattttcatcgtccctctcccatcatttttctctttccctcgtcacgcacttatc
tctgtcactatgagattcttatcttct


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
ACCATTTCATAATACTCAAGTTGCA 453 87 14 Antisense
GTTGCACTATCATATGCGTCTCGTC 491 439 33 Antisense
GCGTCTCGTCAATAGCTAATTCCAA 20 291 48 Antisense
TCCAAACATTTTCTAGGCCGGTCTA 571 585 68 Antisense
GGCCGGTCTAAGCCGTTGAACGTGC 354 537 83 Antisense
GTTGAACGTGCAGTCCGTTTCCACG 163 437 97 Antisense
CCAACCAACTGCATTCTTGTCCAAT 334 277 155 Antisense
ACCAACTGCATTCTTGTCCAATATG 212 85 158 Antisense
GTCCAATATGCACAATTTTTCTTTT 487 477 173 Antisense
ACGCACTTATCTCTGTCACTATGAG 9 93 243 Antisense
TGTCACTATGAGATTCTTATCTTCT 466 559 256 Antisense
Sequence Source Affymetrix Proprietary Database