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[174854_at]

Description CEK050D8R_rc /REP_DB=TREMBL Accession /GB=D64379 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk50d8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 08      4grp_high_8_8 > 49      6grp_highest_nonorm > 50      6grp_total > 04      4grp_total > 62      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 955
1 2 3 4 range
A 1030.4
(3)
P 		369.6
(-1.3)
P 		547
(-0.1)
P 		108.5
(-3)
M 		922
B 766.1
(3)
P 		384.5
(-0.3)
P 		583.9
(1.4)
P 		75.9
(-3)
A 		690
C 959.6
(3)
P 		465.2
(-0.7)
P 		562.9
(0.1)
P 		148.1
(-3)
P 		812
avr 918.7
(3) 		406.4
(-0.8) 		564.6
(0.4) 		110.8
(-3) 		808

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 686
1 2 3 4 5 6 range
A 866
(3)
P 		450.6
(-0.8)
P 		438.4
(-0.9)
P 		373
(-1.5)
P 		599.8
(0.6)
P 		209.1
(-3)
P 		657
B 745.9
(3)
P 		553.5
(0.8)
P 		298
(-2.2)
P 		551.2
(0.7)
P 		436
(-0.6)
P 		227.7
(-3)
P 		518
C 475.6
(3)
P 		428.7
(2)
P 		277.2
(-1)
P 		452.1
(2.5)
P 		376.7
(1)
P 		180.5
(-3)
P 		295
avr 695.8
(3) 		477.6
(0.3) 		337.9
(-1.4) 		458.8
(0.1) 		470.8
(0.2) 		205.8
(-3) 		490
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 359
1 2 3 4 range
A 375.2
(3)
P 		80.2
(-1.9)
P 		143
(-0.9)
P 		15.9
(-3)
M 		359
B 244.3
(3)
P 		95
(-0.9)
P 		135.3
(0.1)
P 		16.2
(-3)
A 		228
C 317.6
(3)
P 		133.8
(-0.8)
P 		196.2
(0.5)
P 		25
(-3)
P 		293
avr 312.4
(3) 		103
(-1.3) 		158.2
(-0.2) 		19
(-3) 		293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 159.6
(2)
P 		160.3
(2)
P 		95.5
(-0.9)
P 		94.6
(-1)
P 		181
(3)
P 		50.9
(-3)
P 		130
B 259.5
(3)
P 		184.2
(0.6)
P 		72.4
(-3)
P 		146
(-0.6)
P 		177.1
(0.4)
P 		84.9
(-2.6)
P 		187
C 176.3
(3)
P 		130.8
(0.6)
P 		70.5
(-2.5)
P 		90.9
(-1.4)
P 		121.9
(0.2)
P 		60.4
(-3)
P 		116
avr 198.5
(3) 		158.4
(1.2) 		79.5
(-2.4) 		110.5
(-1) 		160
(1.3) 		65.4
(-3) 		133
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174854_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21790
Sequence Type Exemplar sequence
Representative Public ID CEK050D8R NCBI
Target Description CEK050D8R_rc /REP_DB=TREMBL Accession /GB=D64379 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk50d8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073279(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073279 NCBI Caenorhabditis elegans Y32F6A.4 (Y32F6A.4) mRNA, complete cds. 11/11 None
Y32F6A.4 ENSEMBL cdna:known chromosome:CEL140:V:10431312:10436186:-1 gene:Y32F6A.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010891 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10431333-10431607(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32F6A.4
Entrez Gene 179456 Entrez gene
SwissProt Q8I4E4 EMBL-EBI
WormBase CE32009 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250161_AT amino acid transporter family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD54163 Hypothetical protein Y32F6A.4 [Caenorhabditis elegans] ref|NP_505680.2| Y32F6A.4 [Caenorhabditis elegans] 0.0
blast CAE64816 Hypothetical protein CBG09610 [Caenorhabditis briggsae] 0.0
blast CAE72727 Hypothetical protein CBG19965 [Caenorhabditis briggsae] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 6.7E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505680.2 11 54-76,86-108,142-161,176-198,205-227,242-264,281-303,318-340,361-383,388-410,441-463
Y32F6A.4 11 54-76,86-108,142-161,176-198,205-227,242-264,281-303,318-340,361-383,388-410,441-463
Sequence
Target Sequence 
>C. ELEGANS:174854_AT
gaaaatgtagtgacccccaattgcatttctatagctaaaattgatttttctctttgtggc
tttaatttttccattcgatctttaacttctctccagaagaagctgtgcaattgttttcta
ttatttttcattttctctgtacaaattttggattaatttgtattcctttccgttcacatg
tataga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAATGTAGTGACCCCCAATTGCA 476 353 32 Antisense
ACCCCCAATTGCATTTCTATAGCTA 519 87 44 Antisense
GATTTTTCTCTTTGTGGCTTTAATT 214 429 74 Antisense
GTGGCTTTAATTTTTCCATTCGATC 374 491 87 Antisense
TTCCATTCGATCTTTAACTTCTCTC 247 699 100 Antisense
TTCGATCTTTAACTTCTCTCCAGAA 573 695 105 Antisense
CTTTAACTTCTCTCCAGAAGAAGCT 621 217 111 Antisense
AGAAGAAGCTGTGCAATTGTTTTCT 573 73 126 Antisense
TTTCATTTTCTCTGTACAAATTTTG 90 673 157 Antisense
TAATTTGTATTCCTTTCCGTTCACA 41 633 185 Antisense
ATTCCTTTCCGTTCACATGTATAGA 193 9 193 Antisense
Sequence Source Affymetrix Proprietary Database