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[174853_at]

Description CEK071D6R_rc /REP_DB=TREMBL Accession /GB=D65948 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk71d6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 39      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 76      6grp_total > 29      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 892
1 2 3 4 range
A 1565.3
(3)
P 		673.7
(-3)
P 		1100.1
(-0.1)
P 		734.7
(-2.6)
P 		892
B 970.3
(-2.7)
P 		1099.7
(-0.1)
P 		956.5
(-3)
P 		1251.2
(3)
P 		295
C 1509.3
(3)
P 		982
(-3)
P 		1096
(-1.7)
P 		1177.5
(-0.8)
P 		527
avr 1348.3
(3) 		918.5
(-3) 		1050.9
(-1.2) 		1054.5
(-1.1) 		430

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1153
1 2 3 4 5 6 range
A 1209.6
(2.4)
P 		946.2
(-0.4)
P 		1009.8
(0.3)
P 		694.2
(-3)
P 		1179.1
(2.1)
P 		1269.5
(3)
P 		575
B 1354.4
(2.7)
P 		766.9
(-2.2)
P 		746.2
(-2.4)
P 		979.8
(-0.4)
P 		670
(-3)
P 		1392.8
(3)
P 		723
C 1746.1
(3)
P 		593.5
(-3)
P 		1238.8
(0.4)
P 		708.3
(-2.4)
P 		1560.8
(2)
P 		819
(-1.8)
P 		1153
avr 1436.7
(3) 		768.9
(-3) 		998.3
(-0.9) 		794.1
(-2.8) 		1136.6
(0.3) 		1160.4
(0.5) 		668
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 462
1 2 3 4 range
A 569.9
(3)
P 		146.2
(-2.5)
P 		287.6
(-0.7)
P 		107.5
(-3)
P 		462
B 309.4
(3)
P 		271.6
(0.4)
P 		221.6
(-3)
P 		266.6
(0.1)
P 		88
C 499.6
(3)
P 		282.3
(-1.3)
P 		382
(0.7)
P 		198.5
(-3)
P 		301
avr 459.6
(3) 		233.4
(-2.1) 		297.1
(-0.6) 		190.9
(-3) 		269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 223
(-1.4)
P 		336.5
(2.4)
P 		219.9
(-1.5)
P 		176.1
(-3)
P 		355.8
(3)
P 		309.1
(1.4)
P 		180
B 471.3
(2.1)
P 		255.3
(-1.7)
P 		181.2
(-3)
P 		259.5
(-1.6)
P 		272.2
(-1.4)
P 		519.4
(3)
P 		338
C 647.2
(3)
P 		181.1
(-2.5)
P 		315.3
(-0.9)
P 		142.4
(-3)
P 		505.1
(1.3)
P 		274.2
(-1.4)
P 		505
avr 447.2
(3) 		257.6
(-1.5) 		238.8
(-1.9) 		192.7
(-3) 		377.7
(1.4) 		367.6
(1.1) 		255
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174853_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20910
Sequence Type Exemplar sequence
Representative Public ID CEK071D6R NCBI
Target Description CEK071D6R_rc /REP_DB=TREMBL Accession /GB=D65948 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk71d6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066744(11), NM_181913(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066744 NCBI Caenorhabditis elegans Tousled-Like Kinase family member (tlk-1) (tlk-1) mRNA, complete cds. 11/11 None
NM_181913 NCBI Caenorhabditis elegans Tousled-Like Kinase family member (tlk-1) (tlk-1) mRNA, complete cds. 11/11 None
C07A9.3a ENSEMBL cdna:known chromosome:CEL140:III:9699777:9710192:1 gene:C07A9.3 11/11 A 
C07A9.3b ENSEMBL cdna:known chromosome:CEL140:III:9699890:9710164:1 gene:C07A9.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9709769-9710178(+) 91.2 100.0
Public Domain and Genome References
Ensembl C07A9.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA82347 Hypothetical protein C07A9.3a [Caenorhabditis elegans] ref|NP_499145.2| Tousled-Like Kinase family member (tlk-1) [Caenorhabditis elegans] gb|AAR18092.1| Tousled-like kinase [Caenorhabditis elegans] 0.0
blast H88562 protein C07A9.3 [imported] - Caenorhabditis elegans 0.0
blast CAD45580 Hypothetical protein C07A9.3b [Caenorhabditis elegans] ref|NP_871642.2| Tousled-Like Kinase family member (tlk-1) [Caenorhabditis elegans] sp|P34314|TLK1_CAEEL Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase 1) 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.12 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CMK2 8.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-58
Sequence
Target Sequence 
>C. ELEGANS:174853_AT
gttccaacaaatcgacatgacatatcccacccagatcccgcgatacattcttttcttcct
caatcgtctctctcttcacacttttcgttccccttccatttttttgttgatttgtttctc
tttttgtaataataatgtatcggacgtcgtcgccctccccaatcccctttctttatttat
aagacgcttctagcaatatatctggagtcatccttctactcttgcttccccccaccacca
ccatctattcacatcctcgtttcaccaatttccttcagttgtacatttgtttagtaaaag
gactgcttacaatgtattgaaacgcgttgaaaggtata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCCAACAAATCGACATGACATAT 668 439 36 Antisense
CAAATCGACATGACATATCCCACCC 664 193 43 Antisense
ATAATGTATCGGACGTCGTCGCCCT 348 25 167 Antisense
CTTTCTTTATTTATAAGACGCTTCT 666 219 202 Antisense
GACGCTTCTAGCAATATATCTGGAG 598 379 218 Antisense
ATATCTGGAGTCATCCTTCTACTCT 245 21 233 Antisense
GTTTCACCAATTTCCTTCAGTTGTA 21 449 294 Antisense
ACCAATTTCCTTCAGTTGTACATTT 146 83 299 Antisense
TTCCTTCAGTTGTACATTTGTTTAG 179 697 305 Antisense
AAAAGGACTGCTTACAATGTATTGA 502 131 331 Antisense
GTATTGAAACGCGTTGAAAGGTATA 447 451 349 Antisense
Sequence Source Affymetrix Proprietary Database