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[174849_at] ATP-dependent RNA helicase like

Description CEK016E2R_rc /REP_DB=TREMBL Accession /GB=D27646 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk16e2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 18      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 10      6grp_total > 18      4grp_total > 46      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 761
1 2 3 4 range
A 1694.3
(3)
P 		932.9
(-3)
P 		1099.4
(-1.7)
P 		1046
(-2.1)
P 		761
B 1661.8
(3)
P 		1430.7
(-0.1)
P 		1212.4
(-3)
P 		1343.8
(-1.2)
P 		449
C 1606.3
(3)
P 		1470.5
(1.3)
P 		1308.3
(-0.8)
P 		1137
(-3)
P 		469
avr 1654.1
(3) 		1278
(-1.7) 		1206.7
(-2.6) 		1175.6
(-3) 		479

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1095
1 2 3 4 5 6 range
A 2054.7
(3)
P 		1389.8
(-0.6)
P 		1015.9
(-2.7)
P 		959.9
(-3)
P 		1529.8
(0.1)
P 		1258.5
(-1.4)
P 		1095
B 2007.6
(3)
P 		1388.2
(-0.9)
P 		1060.1
(-3)
P 		1435.5
(-0.6)
P 		1094.7
(-2.8)
P 		1264.6
(-1.7)
P 		948
C 1629.9
(3)
P 		1233.8
(-1.1)
P 		1176.4
(-1.7)
P 		1123.7
(-2.2)
P 		1048.1
(-3)
P 		1100
(-2.5)
P 		582
avr 1897.4
(3) 		1337.3
(-1.1) 		1084.1
(-3) 		1173
(-2.3) 		1224.2
(-2) 		1207.7
(-2.1) 		813
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 464
1 2 3 4 range
A 616.9
(3)
P 		202.5
(-2.4)
P 		287.4
(-1.3)
P 		153.1
(-3)
P 		464
B 529.9
(3)
P 		353.4
(-1.3)
P 		280.9
(-3)
P 		286.4
(-2.9)
P 		249
C 531.7
(3)
P 		422.8
(1.1)
P 		456
(1.7)
P 		191.7
(-3)
P 		340
avr 559.5
(3) 		326.2
(-1) 		341.4
(-0.7) 		210.4
(-3) 		349

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 477
1 2 3 4 5 6 range
A 378.8
(0.5)
P 		494.3
(3)
P 		221.3
(-3)
P 		243.5
(-2.5)
P 		461.6
(2.3)
P 		306.4
(-1.1)
P 		273
B 698.5
(3)
P 		462.1
(-0.2)
P 		257.4
(-3)
P 		380.2
(-1.3)
P 		444.7
(-0.5)
P 		471.6
(-0.1)
P 		441
C 604.1
(3)
P 		376.5
(-0.6)
P 		299.4
(-1.8)
P 		226
(-3)
P 		339.2
(-1.2)
P 		368.2
(-0.7)
P 		378
avr 560.5
(3) 		444.3
(0.7) 		259.4
(-3) 		283.2
(-2.5) 		415.2
(0.1) 		382.1
(-0.6) 		301
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174849_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21836
Sequence Type Exemplar sequence
Representative Public ID CEK016E2R NCBI
Target Description CEK016E2R_rc /REP_DB=TREMBL Accession /GB=D27646 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk16e2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073982(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073982 NCBI Caenorhabditis elegans F58G11.2 (F58G11.2) mRNA, complete cds. 11/11 None
F58G11.2 ENSEMBL cdna:known chromosome:CEL140:V:13667993:13671865:1 gene:F58G11.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13671472-13671855(+) 99.74 99.74
Public Domain and Genome References
Gene Title ATP-dependent RNA helicase like
Ensembl F58G11.2
Entrez Gene 179855 Entrez gene
SwissProt P90897 EMBL-EBI
WormBase CE11402 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03153 Hypothetical protein F58G11.2 [Caenorhabditis elegans] ref|NP_506383.1| F58G11.2 [Caenorhabditis elegans] 0.0
blast CAE66266 Hypothetical protein CBG11510 [Caenorhabditis briggsae] 1.0E-140
blast AAT12450 vasa protein [Copidosoma floridanum] gb|AAT11555.1| vasa-like protein [Copidosoma floridanum] 7.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.0E-28
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.1E-60
Sequence
Target Sequence 
>C. ELEGANS:174849_AT
atttcgagacatcgcttcacaccattctatcgtattttcgtaattatcaattctggcatg
atcaatcagtcaattgaacatggtgatttatgtgatgtctcgaaaattttcaaattcacg
acaccgcattcttttatttgttttgtaaatcttctgttaagtattgtttccgatcccgat
atttacttactcgtattccgtattccaccgtatccccgtaccttattttgactatctatt
tctttatgaattttgtattcagtacctattaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCGAGACATCGCTTCACACCAT 183 17 90 Antisense
TCACACCATTCTATCGTATTTTCGT 502 627 106 Antisense
AATTCTGGCATGATCAATCAGTCAA 437 177 138 Antisense
ATTTTCAAATTCACGACACCGCATT 118 15 195 Antisense
GACACCGCATTCTTTTATTTGTTTT 534 367 209 Antisense
TGTTAAGTATTGTTTCCGATCCCGA 576 561 244 Antisense
TTCCGATCCCGATATTTACTTACTC 522 453 257 Antisense
TACTTACTCGTATTCCGTATTCCAC 626 645 273 Antisense
TATCCCCGTACCTTATTTTGACTAT 162 653 300 Antisense
TTTTGACTATCTATTTCTTTATGAA 12 679 315 Antisense
GAATTTTGTATTCAGTACCTATTAA 554 323 337 Antisense
Sequence Source Affymetrix Proprietary Database