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[174839_at]

Description CEK019D1R_rc /REP_DB=TREMBL Accession /GB=D32601 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk19d1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 78      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 89      6grp_total > 59      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2910
1 2 3 4 range
A 1121.9
(-2.2)
P 		868.5
(-2.8)
P 		760.7
(-3)
P 		3444.7
(3)
P 		2684
B 1080.7
(-2.7)
P 		1015.4
(-2.9)
P 		947.5
(-3)
P 		3670.7
(3)
P 		2723
C 929.4
(-2.6)
P 		780.6
(-3)
P 		799.1
(-3)
P 		3266.9
(3)
P 		2486
avr 1044
(-2.5) 		888.2
(-2.9) 		835.8
(-3) 		3460.8
(3) 		2625

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2170
1 2 3 4 5 6 range
A 1626.8
(-0.1)
P 		935.8
(-2.2)
P 		670.4
(-3)
P 		741.5
(-2.8)
P 		1781.6
(0.3)
P 		2668.7
(3)
P 		1998
B 1847.2
(0.2)
P 		1095.8
(-2)
P 		739.2
(-3)
P 		1076.4
(-2)
P 		1258.3
(-1.5)
P 		2840.3
(3)
P 		2101
C 1397.1
(-0.9)
P 		1037
(-2.1)
P 		753.8
(-3)
P 		888.8
(-2.6)
P 		1178.7
(-1.6)
P 		2602.3
(3)
P 		1849
avr 1623.7
(-0.3) 		1022.9
(-2.1) 		721.1
(-3) 		902.2
(-2.5) 		1406.2
(-0.9) 		2703.8
(3) 		1983
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 594
1 2 3 4 range
A 408.5
(1.2)
P 		188.5
(-3)
P 		198.9
(-2.8)
P 		504.2
(3)
P 		316
B 344.6
(-1.7)
P 		250.8
(-2.7)
P 		219.6
(-3)
P 		782.2
(3)
P 		563
C 307.7
(-1.5)
P 		224.4
(-3)
P 		278.5
(-2)
P 		550.9
(3)
P 		327
avr 353.6
(-1) 		221.2
(-3) 		232.3
(-2.8) 		612.4
(3) 		391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 913
1 2 3 4 5 6 range
A 299.9
(-1.2)
P 		332.8
(-0.8)
P 		146
(-3)
P 		188.1
(-2.5)
P 		537.6
(1.7)
P 		649.8
(3)
P 		504
B 642.7
(0.2)
P 		364.7
(-1.7)
P 		179.5
(-3)
P 		285.1
(-2.3)
P 		511.2
(-0.7)
P 		1059.1
(3)
P 		880
C 517.8
(-0.1)
P 		316.4
(-1.8)
P 		191.9
(-2.9)
P 		178.7
(-3)
P 		381.4
(-1.2)
P 		871.2
(3)
P 		693
avr 486.8
(-0.3) 		338
(-1.6) 		172.5
(-3) 		217.3
(-2.6) 		476.7
(-0.3) 		860
(3) 		688
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174839_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21654
Sequence Type Exemplar sequence
Representative Public ID CEK019D1R NCBI
Target Description CEK019D1R_rc /REP_DB=TREMBL Accession /GB=D32601 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk19d1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027976(11), NM_001027979(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027976 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-7) (fkh-7) mRNA, complete cds. 11/11 None
NM_001027979 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-7) (fkh-7) mRNA, complete cds. 11/11 None
F26D12.1a ENSEMBL cdna:known chromosome:CEL140:IV:5584670:5610349:1 gene:F26D12.1 11/11 A 
F26D12.1d ENSEMBL cdna:known chromosome:CEL140:IV:5609394:5610349:1 gene:F26D12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177340 4 2 NM_001027976 174839_at (11), 187837_at (11)
NM_001027979 174839_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5609979-5610339(+) 100.0 100.0
Public Domain and Genome References
Ensembl F26D12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR85907 Forkhead transcription factor family protein 7, isoform a [Caenorhabditis elegans] ref|NP_001023147.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 0.0
blast AAR85908 Forkhead transcription factor family protein 7, isoform b [Caenorhabditis elegans] ref|NP_001023148.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 0.0
blast CAE72690 Hypothetical protein CBG19911 [Caenorhabditis briggsae] 0.0
blast AAO91733 Forkhead transcription factor family protein 7, isoform d [Caenorhabditis elegans] ref|NP_001023150.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 3.0E-42
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.00000001268431E-29
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.8E-29
Sequence
Target Sequence 
>C. ELEGANS:174839_AT
gacactacacatttgatctactaccatcttccaacacactttcccgccttttttctctcg
ttgtcagggtctctccaacatccttctaatctctcgattcatctcccattacagtagcgt
tgttttctttgtttgtgattcgccctgtctgaaactgtttcagaaactcttaatattcac
tgaaattacctccttctcccattttgcccacactttgtattttatgcgcaagttgtgatt
tgtgtgatggtttttactttttcaacaatttttcgcccatttcctcccattttctacttt
ctttttatcattga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACTACACATTTGATCTACTACC 433 367 14 Antisense
TTTGATCTACTACCATCTTCCAACA 299 667 25 Antisense
ATTCATCTCCCATTACAGTAGCGTT 562 9 110 Antisense
CAGTAGCGTTGTTTTCTTTGTTTGT 319 207 125 Antisense
TGTTTTCTTTGTTTGTGATTCGCCC 642 561 134 Antisense
TTCGCCCTGTCTGAAACTGTTTCAG 317 697 152 Antisense
TATTCACTGAAATTACCTCCTTCTC 35 661 187 Antisense
CCATTTTGCCCACACTTTGTATTTT 628 265 212 Antisense
GCCCACACTTTGTATTTTATGCGCA 172 287 219 Antisense
TGTGATGGTTTTTACTTTTTCAACA 534 555 256 Antisense
TTTTCTACTTTCTTTTTATCATTGA 73 677 303 Antisense
Sequence Source Affymetrix Proprietary Database