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[174835_at] man(9)-alpha-mannosidase

Description CEK020E7R_rc /REP_DB=TREMBL Accession /GB=D32718 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk20e7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 41      6grp_total > 26      4grp_total > 55      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 391
1 2 3 4 range
A 728.1
(3)
P 		628.2
(0.1)
P 		528.9
(-2.8)
P 		523.2
(-3)
P 		205
B 796.8
(3)
P 		562.6
(-3)
P 		744.7
(1.7)
P 		676.9
(-0.1)
P 		234
C 914.1
(3)
P 		537.5
(-3)
P 		783.9
(0.9)
P 		685.3
(-0.6)
P 		377
avr 813
(3) 		576.1
(-3) 		685.8
(-0.2) 		628.5
(-1.7) 		237

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 438
1 2 3 4 5 6 range
A 806
(0.4)
P 		814.3
(0.6)
P 		554.4
(-3)
P 		635.9
(-1.9)
P 		992.8
(3)
P 		690.3
(-1.1)
P 		438
B 891.1
(2.4)
P 		840.6
(1.6)
P 		927.9
(3)
P 		600.5
(-2.4)
P 		561.1
(-3)
P 		715.7
(-0.5)
P 		367
C 849.3
(1.3)
P 		954
(3)
P 		576.1
(-3)
P 		799.6
(0.5)
P 		584
(-2.9)
P 		683.9
(-1.3)
P 		378
avr 848.8
(2.3) 		869.6
(3) 		686.1
(-2.8) 		678.7
(-3) 		712.6
(-1.9) 		696.6
(-2.4) 		191
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 226
1 2 3 4 range
A 265.1
(3)
P 		136.4
(-1.1)
P 		138.3
(-1)
P 		76.6
(-3)
P 		189
B 254.1
(3)
P 		138.9
(-3)
P 		172.6
(-1.2)
P 		144.2
(-2.7)
P 		115
C 302.6
(3)
P 		154.5
(-1.7)
P 		273.2
(2.1)
P 		115.5
(-3)
P 		187
avr 273.9
(3) 		143.3
(-1.8) 		194.7
(0.1) 		112.1
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 148.6
(-2.1)
P 		289.6
(2.7)
P 		120.7
(-3)
P 		161.3
(-1.6)
P 		299.6
(3)
P 		168.1
(-1.4)
P 		179
B 310.1
(3)
P 		279.8
(1.8)
P 		225.3
(-0.4)
P 		159
(-3)
P 		228
(-0.3)
P 		266.9
(1.3)
P 		151
C 314.8
(3)
P 		291.1
(2.2)
P 		146.6
(-3)
P 		160.8
(-2.5)
P 		189
(-1.5)
P 		228.9
(-0.1)
P 		168
avr 257.8
(1.6) 		286.8
(3) 		164.2
(-2.8) 		160.4
(-3) 		238.9
(0.7) 		221.3
(-0.1) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174835_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22156
Sequence Type Exemplar sequence
Representative Public ID CEK020E7R NCBI
Target Description CEK020E7R_rc /REP_DB=TREMBL Accession /GB=D32718 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk20e7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077270(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077270 NCBI Caenorhabditis elegans ZC506.1 (man(9)-alpha-mannosidase) mRNA, complete cds. 11/11 None
ZC506.1 ENSEMBL cdna:known chromosome:CEL140:X:9958892:9964665:1 gene:ZC506.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9964312-9964655(+) 100.0 100.0
Public Domain and Genome References
Gene Title man(9)-alpha-mannosidase
Ensembl ZC506.1
Entrez Gene 181210 Entrez gene
SwissProt Q09641 EMBL-EBI
WormBase CE37461 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.20827.1.S1_AT similar to Putative alpha-mannosidase C1orf22 cfa
DROSOPHILA_2:1630203_AT dm
DROSGENOME1:146315_AT dm
CHICKEN:GGA.10298.1.S1_S_AT similar to Putative alpha-mannosidase C1orf22 gga
CHICKEN:GGAAFFX.26310.1.S1_AT similar to Putative alpha-mannosidase C1orf22 gga
HG-U133_PLUS_2:220342_X_AT chromosome 1 open reading frame 22 hs
HG-U133A:220342_X_AT chromosome 1 open reading frame 22 hs
HG-U133A_2:220342_X_AT chromosome 1 open reading frame 22 hs
HU35KSUBA:RC_AA256323_AT chromosome 1 open reading frame 22 hs
HU35KSUBB:RC_AA179387_AT chromosome 1 open reading frame 22 hs
HG-U133A:220926_S_AT chromosome 1 open reading frame 22 hs
HG-U133_PLUS_2:220926_S_AT chromosome 1 open reading frame 22 hs
HG-U133A_2:220926_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:G8922223_3P_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:G13376781_3P_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:HS.301997.1.S1_3P_S_AT chromosome 1 open reading frame 22 hs
HG-U133_PLUS_2:223243_S_AT chromosome 1 open reading frame 22 hs
HG-U133B:223243_S_AT chromosome 1 open reading frame 22 hs
HG-U95B:59633_AT chromosome 1 open reading frame 22 hs
HG-U95C:65608_AT chromosome 1 open reading frame 22 hs
HG-U95E:90857_AT chromosome 1 open reading frame 22 hs
HG-U95C:56528_R_AT Chromosome 1 open reading frame 22 hs
MG-U74AV2:104114_AT RIKEN cDNA 2310050N11 gene mm
MOE430A:1460401_AT RIKEN cDNA 2310050N11 gene mm
MOUSE430A_2:1460401_AT RIKEN cDNA 2310050N11 gene mm
MOUSE430_2:1460401_AT RIKEN cDNA 2310050N11 gene mm
MG-U74BV2:105544_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MG-U74BV2:110546_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MOE430B:1459133_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MOUSE430_2:1459133_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87371 Hypothetical protein ZC506.1 [Caenorhabditis elegans] ref|NP_509671.3| ZC506.1 [Caenorhabditis elegans] 0.0
blast CAE58760 Hypothetical protein CBG01952 [Caenorhabditis briggsae] 0.0
blast XP_193956 PREDICTED: hypothetical protein LOC66967 [Mus musculus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Pfam IPR003137 EMBL-EBI Protease-associated PA 1.6E-29
Sequence
Target Sequence 
>C. ELEGANS:174835_AT
cacaagccatacatgttccgtttctaaacttgggttaattgtctgtttctttcaaaaatt
catatacatataacatttgataatctattcaaaagcaacattcatgcctttagcaatttc
ctgtaaggtgataaaatgcgggttaaaaatttgtttccttttttcttctgttgtttatat
atttactttgttcaccgtttcatcgtaagcccactgacgacttcttcaacttcctccaag
caaatgtgtatttaaatcaacaaaatgtgtgtagaatttacttgtaaatgtttacttttc
aatggttcaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACAAGCCATACATGTTCCGTTTCT 300 193 14 Antisense
TACATGTTCCGTTTCTAAACTTGGG 137 645 23 Antisense
TTGGGTTAATTGTCTGTTTCTTTCA 502 707 43 Antisense
AAGCAACATTCATGCCTTTAGCAAT 550 151 106 Antisense
CATGCCTTTAGCAATTTCCTGTAAG 540 209 116 Antisense
ATATTTACTTTGTTCACCGTTTCAT 362 19 192 Antisense
TGTTCACCGTTTCATCGTAAGCCCA 490 557 202 Antisense
ATCGTAAGCCCACTGACGACTTCTT 596 35 215 Antisense
TTCTTCAACTTCCTCCAAGCAAATG 577 675 235 Antisense
CAACTTCCTCCAAGCAAATGTGTAT 294 189 240 Antisense
ATGTTTACTTTTCAATGGTTCACCG 444 13 301 Antisense
Sequence Source Affymetrix Proprietary Database