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[174823_at] twt1 like protein

Description CEK025C9R_rc /REP_DB=TREMBL Accession /GB=D33039 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk25c9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 12      6grp_total > 24      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3207
1 2 3 4 range
A 4558.3
(3)
P 		2097.1
(-2.2)
P 		1698.1
(-3)
P 		2855.4
(-0.6)
P 		2860
B 4820.7
(3)
P 		1838.2
(-2.7)
P 		1696.2
(-3)
P 		2907.4
(-0.7)
P 		3125
C 4903.4
(3)
P 		1788.7
(-3)
P 		1776.8
(-3)
P 		2487.6
(-1.6)
P 		3127
avr 4760.8
(3) 		1908
(-2.6) 		1723.7
(-3) 		2750.1
(-1) 		3037

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1826
1 2 3 4 5 6 range
A 2787.9
(3)
P 		2537.3
(2.2)
P 		2069
(0.6)
P 		1273.8
(-2.1)
P 		1647.2
(-0.8)
P 		998.9
(-3)
P 		1789
B 2550.5
(2.4)
P 		2722
(3)
P 		2585.3
(2.5)
P 		1766.4
(-0.3)
P 		1442.3
(-1.4)
P 		984.7
(-3)
P 		1737
C 2700
(3)
P 		2544
(2.5)
P 		1896.6
(0.2)
P 		1544.1
(-1)
P 		1546.1
(-1)
P 		962.4
(-3)
P 		1738
avr 2679.5
(3) 		2601.1
(2.7) 		2183.6
(1.2) 		1528.1
(-1.1) 		1545.2
(-1) 		982
(-3) 		1698
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1267
1 2 3 4 range
A 1659.7
(3)
P 		455.2
(-2.8)
P 		443.9
(-2.9)
P 		418
(-3)
P 		1242
B 1537.3
(3)
P 		454
(-2.7)
P 		393
(-3)
P 		619.6
(-1.8)
P 		1144
C 1623.2
(3)
P 		514.3
(-2.5)
P 		619.3
(-2)
P 		419.5
(-3)
P 		1204
avr 1606.7
(3) 		474.5
(-3) 		485.4
(-2.9) 		485.7
(-2.9) 		1132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 758
1 2 3 4 5 6 range
A 513.9
(-0.5)
P 		902.4
(3)
P 		450.6
(-1.1)
P 		323.1
(-2.3)
P 		497
(-0.7)
P 		243.2
(-3)
P 		659
B 887.5
(2.8)
P 		906.1
(3)
P 		627.8
(-0.1)
P 		467.9
(-1.9)
P 		586
(-0.6)
P 		367.2
(-3)
P 		539
C 1000.8
(3)
P 		776.3
(1)
P 		482.7
(-1.5)
P 		310.5
(-3)
P 		500.3
(-1.4)
P 		322.2
(-2.9)
P 		690
avr 800.7
(2.3) 		861.6
(3) 		520.4
(-0.7) 		367.2
(-2.4) 		527.8
(-0.6) 		310.9
(-3) 		551
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174823_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22281
Sequence Type Exemplar sequence
Representative Public ID CEK025C9R NCBI
Target Description CEK025C9R_rc /REP_DB=TREMBL Accession /GB=D33039 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk25c9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077743(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077743 NCBI Caenorhabditis elegans Branched Chain AminoTransferase family member (bcat-1) (bcat-1) mRNA, complete cds. 11/11 None
K02A4.1.2 ENSEMBL cdna:known chromosome:CEL140:X:12877397:12880248:1 gene:K02A4.1 11/11 None
K02A4.1.1 ENSEMBL cdna:known chromosome:CEL140:X:12877399:12880248:1 gene:K02A4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12879841-12880228(+) 100.0 100.0
Public Domain and Genome References
Gene Title twt1 like protein
Gene Symbol bcat-1
Ensembl K02A4.1
Entrez Gene 181423 Entrez gene
SwissProt P54688 EMBL-EBI
WormBase CE03457 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264524_AT branched-chain amino acid aminotransferase 2 / branched-chain amino acid transaminase 2 (BCAT2) at
ATGENOME1:14052_AT branched-chain amino acid aminotransferase 2 / branched-chain amino acid transaminase 2 (BCAT2) at
CANINE_2:CFAAFFX.17875.1.S1_AT similar to branched chain aminotransferase 1, cytosolic cfa
CANINE_2:CFAAFFX.17877.1.S1_AT similar to branched chain aminotransferase 1, cytosolic cfa
CANINE_2:CFAAFFX.17877.1.S1_S_AT similar to branched chain aminotransferase 1, cytosolic cfa
DROSOPHILA_2:1623770_AT dm
DROSGENOME1:145006_AT dm
CHICKEN:GGA.2408.1.S1_AT similar to branched chain aminotransferase 1, cytosolic gga
HG-U133_PLUS_2:214452_AT branched chain aminotransferase 1, cytosolic hs
HG-U133A_2:214452_AT branched chain aminotransferase 1, cytosolic hs
HG-FOCUS:214452_AT branched chain aminotransferase 1, cytosolic hs
HG-U133A:214452_AT branched chain aminotransferase 1, cytosolic hs
HU35KSUBA:RC_AA423987_AT branched chain aminotransferase 1, cytosolic hs
HUGENEFL:U21551_AT branched chain aminotransferase 1, cytosolic hs
U133_X3P:HS.7567.0.S1_3P_AT branched chain aminotransferase 1, cytosolic hs
U133_X3P:HS.317432.0.S1_3P_AT branched chain aminotransferase 1, cytosolic hs
U133_X3P:HS.157205.1.S1_3P_AT branched chain aminotransferase 1, cytosolic hs
U133_X3P:HS.157205.0.S1_3P_A_AT branched chain aminotransferase 1, cytosolic hs
HG-U133A_2:214390_S_AT branched chain aminotransferase 1, cytosolic hs
HG-U133_PLUS_2:214390_S_AT branched chain aminotransferase 1, cytosolic hs
HG-U133A:214390_S_AT branched chain aminotransferase 1, cytosolic hs
HG-U133_PLUS_2:225285_AT branched chain aminotransferase 1, cytosolic hs
HG-U133B:225285_AT branched chain aminotransferase 1, cytosolic hs
HG-U133_PLUS_2:226517_AT branched chain aminotransferase 1, cytosolic hs
HG-U133B:226517_AT branched chain aminotransferase 1, cytosolic hs
HG-U95AV2:38201_AT branched chain aminotransferase 1, cytosolic hs
HG-U95B:43829_AT branched chain aminotransferase 1, cytosolic hs
HG-U95B:54904_AT branched chain aminotransferase 1, cytosolic hs
HG-U95E:72960_S_AT branched chain aminotransferase 1, cytosolic hs
HG-U95E:72962_AT branched chain aminotransferase 1, cytosolic hs
HG-U95D:82008_AT branched chain aminotransferase 1, cytosolic hs
HG-U95E:89873_AT branched chain aminotransferase 1, cytosolic hs
HG-U95D:83494_AT Branched chain aminotransferase 1, cytosolic hs
MOE430A:1430111_A_AT branched chain aminotransferase 1, cytosolic mm
MOUSE430_2:1430111_A_AT branched chain aminotransferase 1, cytosolic mm
MOUSE430A_2:1430111_A_AT branched chain aminotransferase 1, cytosolic mm
MU11KSUBB:X17502_S_AT branched chain aminotransferase 1, cytosolic mm
MG-U74AV2:100026_AT branched chain aminotransferase 1, cytosolic mm
MOE430A:1450871_A_AT branched chain aminotransferase 1, cytosolic mm
MOUSE430A_2:1450871_A_AT branched chain aminotransferase 1, cytosolic mm
MOUSE430_2:1450871_A_AT branched chain aminotransferase 1, cytosolic mm
MU19KSUBC:TC35789_S_AT Branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2, mRNA mm
RAT230_2:1369520_A_AT branched chain aminotransferase 1, cytosolic rn
RAE230A:1369520_A_AT branched chain aminotransferase 1, cytosolic rn
RG-U34A:U35774_AT branched chain aminotransferase 1, cytosolic rn
RG-U34B:RC_AA900881_AT branched chain aminotransferase 1, cytosolic rn
RAE230A:1370869_AT branched chain aminotransferase 1, cytosolic rn
RAT230_2:1370869_AT branched chain aminotransferase 1, cytosolic rn
RAE230B:1380353_AT Branched chain aminotransferase 1, cytosolic rn
RAT230_2:1380353_AT Branched chain aminotransferase 1, cytosolic rn
RAE230A:1390726_AT Branched chain aminotransferase 1, cytosolic rn
RAT230_2:1390726_AT Branched chain aminotransferase 1, cytosolic rn
YEAST_2:1777344_AT Cytosolic branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
9081 branched chain family amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4084 branched-chain-amino-acid transaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91805 Hypothetical protein K02A4.1 [Caenorhabditis elegans] ref|NP_510144.1| Branched Chain AminoTransferase family member (bcat-1) [Caenorhabditis elegans] sp|P54688|BCAT_CAEEL Branched-chain-amino-acid aminotransferase, cytosolic (BCAT) (ECA39 protein) 0.0
blast CAE57200 Hypothetical protein CBG00050 [Caenorhabditis briggsae] 0.0
blast AAC47236 ECA39 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001544 EMBL-EBI Aminotransferase, class IV 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174823_AT
tatgagcttttccgaagagctaagctaacgattttctgagttcatatcagaatacgtata
caagtcagctcggcgttgattagatcattcgcctccttattccccttcactaaaactaca
actagttttatagacacatttattgattcgatttcaaatggtatttttttaatttcttct
cttcaatccccctcccaacttccttccaccccctcaaaaagcgctcgtgtcccggtttat
aaatcatgcttcctgctacacttcaccatgtcaaatactcatatgatcatctagaataat
tttttttatgtatatgagaaagtcgttgttagttcaaatggctattcgattgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGAGCTTTTCCGAAGAGCTAAGC 228 657 13 Antisense
GAGCTAAGCTAACGATTTTCTGAGT 354 385 29 Antisense
GTATACAAGTCAGCTCGGCGTTGAT 347 453 68 Antisense
GCGTTGATTAGATCATTCGCCTCCT 529 291 85 Antisense
TCCCCTTCACTAAAACTACAACTAG 653 593 113 Antisense
TAGACACATTTATTGATTCGATTTC 304 653 143 Antisense
CGCTCGTGTCCCGGTTTATAAATCA 531 253 234 Antisense
ATAAATCATGCTTCCTGCTACACTT 222 25 251 Antisense
CTGCTACACTTCACCATGTCAAATA 29 239 265 Antisense
GTCAAATACTCATATGATCATCTAG 374 465 282 Antisense
AGTTCAAATGGCTATTCGATTGCAT 18 63 343 Antisense
Sequence Source Affymetrix Proprietary Database