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[174822_at]

Description CEK056B2R_rc /REP_DB=TREMBL Accession /GB=D65416 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk56b2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 12      4grp_highest_8_8 > 15      4grp_high_8_8 > 21      6grp_highest_nonorm > 36      6grp_total > 33      4grp_total > 10      6grp_cgc > 70      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4534
1 2 3 4 range
A 5312.6
(-3)
P 		6512.3
(-0.4)
P 		5517.7
(-2.6)
P 		8056.8
(3)
P 		2744
B 5278.2
(-3)
P 		5423.7
(-2.6)
P 		5608.8
(-2.1)
P 		7606
(3)
P 		2328
C 4801.5
(-3)
P 		5534.7
(-2)
P 		5347.4
(-2.3)
P 		9335.6
(3)
P 		4534
avr 5130.8
(-3) 		5823.6
(-1.7) 		5491.3
(-2.3) 		8332.8
(3) 		3202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2173
1 2 3 4 5 6 range
A 5209.9
(3)
P 		3926.3
(-2.5)
P 		4328.4
(-0.8)
P 		3925.6
(-2.5)
P 		3881.2
(-2.7)
P 		3801.5
(-3)
P 		1408
B 4924.6
(1.3)
P 		4982.7
(1.5)
P 		5433.3
(3)
P 		3725.7
(-2.6)
P 		3604.2
(-3)
P 		3681.9
(-2.7)
P 		1829
C 3830.3
(-0.6)
P 		4702.6
(3)
P 		4452.3
(2)
P 		4329.1
(1.4)
P 		4480.5
(2.1)
P 		3260.7
(-3)
P 		1442
avr 4654.9
(2.6) 		4537.2
(2) 		4738
(3) 		3993.5
(-0.9) 		3988.6
(-0.9) 		3581.4
(-3) 		1157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 755
1 2 3 4 range
A 1934.3
(3)
P 		1413.6
(-1.1)
P 		1442.4
(-0.9)
P 		1179.4
(-3)
P 		755
B 1683.2
(3)
P 		1339.5
(-2.4)
P 		1299.7
(-3)
P 		1620.9
(2)
P 		384
C 1589.4
(-2.7)
P 		1591.3
(-2.6)
P 		1863.7
(3)
P 		1574.1
(-3)
P 		290
avr 1735.6
(3) 		1448.1
(-3) 		1535.3
(-1.2) 		1458.1
(-2.8) 		288

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 843
1 2 3 4 5 6 range
A 960.4
(-2.6)
P 		1396.4
(3)
P 		942.6
(-2.8)
P 		995.6
(-2.1)
P 		1171.2
(0.1)
P 		925.5
(-3)
P 		471
B 1713.5
(3)
P 		1658.5
(2.5)
P 		1319.4
(-0.3)
P 		986.8
(-3)
P 		1464.4
(0.9)
P 		1373
(0.2)
P 		727
C 1419.7
(2.7)
P 		1434.9
(2.8)
P 		1133.2
(-0.3)
P 		870.6
(-3)
P 		1449.8
(3)
P 		1091.5
(-0.7)
P 		579
avr 1364.5
(1.5) 		1496.6
(3) 		1131.7
(-1) 		951
(-3) 		1361.8
(1.5) 		1130
(-1) 		546
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174822_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20720
Sequence Type Exemplar sequence
Representative Public ID CEK056B2R NCBI
Target Description CEK056B2R_rc /REP_DB=TREMBL Accession /GB=D65416 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk56b2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170974(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170974 NCBI Caenorhabditis elegans VIG (Drosophila Vasa Intronic Gene) ortholog family member (vig-1) (vig-1) mRNA, complete cds. 11/11 None
F56D12.5a.2 ENSEMBL cdna:known chromosome:CEL140:II:1321041:1323206:1 gene:F56D12.5 11/11 None
F56D12.5a.1 ENSEMBL cdna:known chromosome:CEL140:II:1321043:1323206:1 gene:F56D12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_170973 6/11 Cross Hyb Matching Probes None
F56D12.5b 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173512 2 1 NM_170974 174822_at (11), 186328_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1322719-1323056(+) 100.0 100.0
Public Domain and Genome References
Ensembl F56D12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66119 Vig (drosophila vasa intronic gene) ortholog protein 1, isoform a [Caenorhabditis elegans] ref|NP_740971.1| VIG (Drosophila Vasa Intronic Gene) ortholog family member (vig-1) [Caenorhabditis elegans] 8.0E-92
blast CAE62914 Hypothetical protein CBG07107 [Caenorhabditis briggsae] 1.0E-70
blast AAT79349 ALT-1 [Strongyloides ratti] gb|AAT79348.1| ALT-1 [Strongyloides ratti] 3.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR006861 EMBL-EBI Hyaluronan/mRNA binding protein 2.9E-71
Sequence
Target Sequence 
>C. ELEGANS:174822_AT
ttcccccatttagcttgttgttgttatcacctgccttatcgatctctcttctgatgagtt
gccctcgatttggtttgcttgttatctcccgttttttctttaatttttaagccctttgaa
tcccttgatcttccacatacacccaccaagacgacctcttctttaccaaatatttgacac
aacaaaaggtctcaattgtgtcaaggatcaacgaactagtactaggaaactcaatttgtg
ccccttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCCCCATTTAGCTTGTTGTTGTT 439 693 50 Antisense
TAGCTTGTTGTTGTTATCACCTGCC 90 653 60 Antisense
CCTTATCGATCTCTCTTCTGATGAG 357 269 83 Antisense
ATCTCTCTTCTGATGAGTTGCCCTC 435 33 91 Antisense
GATGAGTTGCCCTCGATTTGGTTTG 512 409 102 Antisense
ATTTGGTTTGCTTGTTATCTCCCGT 557 17 117 Antisense
TTTAAGCCCTTTGAATCCCTTGATC 75 559 155 Antisense
CGACCTCTTCTTTACCAAATATTTG 699 249 201 Antisense
AAAGGTCTCAATTGTGTCAAGGATC 236 119 234 Antisense
GTCAAGGATCAACGAACTAGTACTA 511 463 249 Antisense
TAGGAAACTCAATTTGTGCCCCTTC 242 653 272 Antisense
Sequence Source Affymetrix Proprietary Database