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[174815_at] guanylate cyclase

Description CEK040A8R_rc /REP_DB=TREMBL Accession /GB=D34048 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk40a8 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 09      6grp_total > 04      4grp_total > 14      6grp_cgc > 41      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 111.7
(3)
P 		18.8
(-3)
A 		42.7
(-1.5)
A 		74.2
(0.6)
A 		93
B 117.4
(1.9)
P 		54
(-3)
A 		66.9
(-2)
P 		132.2
(3)
P 		78
C 131.6
(0.2)
P 		37.4
(-3)
A 		59
(-2.3)
P 		214.6
(3)
P 		177
avr 120.2
(1.8) 		36.7
(-3) 		56.2
(-1.9) 		140.3
(3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 108.5
(3)
A 		69.4
(-0.8)
P 		76.6
(-0.1)
M 		89.1
(1.1)
P 		93.8
(1.6)
P 		46.7
(-3)
A 		62
B 124.4
(3)
P 		75.8
(-0.2)
P 		61.7
(-1.2)
P 		70.5
(-0.6)
P 		100.2
(1.4)
P 		33.8
(-3)
A 		91
C 62.6
(-0.5)
P 		113.6
(3)
P 		68.4
(-0.1)
P 		63.3
(-0.4)
A 		25.9
(-3)
A 		43.4
(-1.8)
P 		88
avr 98.5
(3) 		86.3
(1.7) 		68.9
(-0.1) 		74.3
(0.5) 		73.3
(0.4) 		41.3
(-3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 40.7
(3)
P 		4.1
(-3)
A 		11.2
(-1.8)
A 		10.9
(-1.9)
A 		37
B 37.4
(3)
P 		13.3
(-3)
A 		15.5
(-2.5)
P 		28.2
(0.7)
P 		24
C 43.6
(3)
P 		10.8
(-3)
A 		20.6
(-1.2)
P 		36.2
(1.6)
P 		33
avr 40.6
(3) 		9.4
(-3) 		15.8
(-1.8) 		25.1
(0) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 20
(0.1)
A 		24.7
(1.7)
P 		16.7
(-1.1)
M 		22.6
(1)
P 		28.3
(3)
P 		11.4
(-3)
A 		17
B 43.3
(3)
P 		25.2
(-0.5)
P 		15
(-2.5)
P 		18.7
(-1.8)
P 		40.7
(2.5)
P 		12.6
(-3)
A 		31
C 23.2
(0.4)
P 		34.7
(3)
P 		17.4
(-0.9)
P 		12.7
(-2)
A 		8.4
(-3)
A 		14.5
(-1.6)
P 		26
avr 28.8
(3) 		28.2
(2.8) 		16.4
(-1.7) 		18
(-1.1) 		25.8
(1.9) 		12.8
(-3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174815_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21851
Sequence Type Exemplar sequence
Representative Public ID CEK040A8R NCBI
Target Description CEK040A8R_rc /REP_DB=TREMBL Accession /GB=D34048 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk40a8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073559(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073559 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-11) (daf-11) mRNA, complete cds. 11/11 None
B0240.3 ENSEMBL cdna:known chromosome:CEL140:V:11720678:11728241:-1 gene:B0240.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11720690-11721058(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol daf-11
Ensembl B0240.3
Entrez Gene 179605 Entrez gene
SwissProt Q8I4N4 EMBL-EBI
WormBase CE05174 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD56284 Hypothetical protein B0240.3 [Caenorhabditis elegans] ref|NP_505960.1| abnormal DAuer Formation family member (daf-11) [Caenorhabditis elegans] 0.0
blast CAE75310 Hypothetical protein CBG23280 [Caenorhabditis briggsae] 0.0
blast CAB05325 Hypothetical protein ZK896.8 [Caenorhabditis elegans] ref|NP_502449.1| Guanylyl CYclase family member (gcy-18) [Caenorhabditis elegans] 3.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 0.21
Pfam IPR000719 EMBL-EBI Protein kinase 8.4E-4
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.0E-53
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505960.1 2 224-243,346-368
B0240.3 2 224-243,346-368
Sequence
Target Sequence 
>C. ELEGANS:174815_AT
agaacatttcgacccagaactacatatttaaaattgattattttgatctccaattttaaa
ttaaagagattttcacgtcacagtaagtttataactcgaaaatgtttttttaatctcaaa
ttatcttcattaaaaaattcaccacttcaaaaaaaacatcaaaaaataaaacattattcc
ttattccactgcacccaagaactttcaaaaattccgtaatcaaagaagccttttttaata
tttttatcttgtacatgccttaatgtttctcttttttgccctccccacatttctctcagt
cttgcttgtttttggtgctgttctttggactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAACATTTCGACCCAGAACTACAT 512 71 19 Antisense
TTCGACCCAGAACTACATATTTAAA 434 695 26 Antisense
TTGATTATTTTGATCTCCAATTTTA 579 705 52 Antisense
GAGATTTTCACGTCACAGTAAGTTT 6 397 84 Antisense
TTCATTAAAAAATTCACCACTTCAA 295 687 144 Antisense
TCCACTGCACCCAAGAACTTTCAAA 365 589 203 Antisense
ATATTTTTATCTTGTACATGCCTTA 452 19 256 Antisense
TGCCTTAATGTTTCTCTTTTTTGCC 578 585 274 Antisense
TTCTCTCAGTCTTGCTTGTTTTTGG 214 691 309 Antisense
GTCTTGCTTGTTTTTGGTGCTGTTC 695 467 317 Antisense
GTTTTTGGTGCTGTTCTTTGGACTT 373 447 326 Antisense
Sequence Source Affymetrix Proprietary Database