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[174804_at]

Description CEK067F3R_rc /REP_DB=TREMBL Accession /GB=D71349 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk67f3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 36      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 30      6grp_total > 26      4grp_total > 53      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1173
1 2 3 4 range
A 1421.2
(2.5)
P 		1505.3
(3)
P 		1137.7
(0.7)
P 		527.1
(-3)
P 		978
B 1699.9
(3)
P 		1382.8
(0.8)
P 		1169
(-0.8)
P 		850.8
(-3)
P 		849
C 1483.9
(3)
P 		1240.7
(0.9)
P 		1206.9
(0.6)
P 		795.9
(-3)
P 		688
avr 1535
(3) 		1376.3
(1.8) 		1171.2
(0.3) 		724.6
(-3) 		810

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 743
1 2 3 4 5 6 range
A 1320.3
(3)
P 		1222.8
(2.2)
P 		862.5
(-0.9)
P 		624.7
(-3)
P 		798.3
(-1.5)
P 		1122.1
(1.3)
P 		696
B 1115.9
(3)
P 		1072.4
(2.5)
P 		755.5
(-1)
P 		763.5
(-0.9)
P 		577.2
(-3)
P 		832.8
(-0.2)
P 		539
C 1194.6
(3)
P 		900
(-0.3)
P 		973.2
(0.5)
P 		665.5
(-3)
P 		675.9
(-2.9)
P 		826
(-1.2)
P 		529
avr 1210.3
(3) 		1065.1
(1.3) 		863.7
(-0.9) 		684.6
(-3) 		683.8
(-3) 		927
(-0.2) 		527
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 465
1 2 3 4 range
A 517.4
(3)
P 		326.8
(0.4)
P 		297.4
(0)
P 		77.2
(-3)
P 		440
B 542.1
(3)
P 		341.5
(-0.3)
P 		270.9
(-1.5)
P 		181.3
(-3)
P 		361
C 491.2
(3)
P 		356.7
(0.7)
P 		420.7
(1.8)
P 		134.2
(-3)
P 		357
avr 516.9
(3) 		341.7
(0.3) 		329.7
(0.1) 		130.9
(-3) 		386

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 309
1 2 3 4 5 6 range
A 243.4
(-1.2)
P 		434.9
(3)
P 		187.8
(-2.4)
P 		158.4
(-3)
P 		240.9
(-1.2)
P 		273.2
(-0.5)
P 		277
B 388.3
(3)
P 		357
(2.1)
P 		183.4
(-3)
P 		202.2
(-2.4)
P 		234.5
(-1.5)
P 		310.6
(0.7)
P 		205
C 442.8
(3)
P 		274.6
(-0.3)
P 		247.7
(-0.8)
P 		133.8
(-3)
P 		218.7
(-1.4)
P 		276.5
(-0.2)
P 		309
avr 358.2
(3) 		355.5
(2.9) 		206.3
(-1.7) 		164.8
(-3) 		231.4
(-0.9) 		286.8
(0.8) 		193
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174804_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20665
Sequence Type Exemplar sequence
Representative Public ID CEK067F3R NCBI
Target Description CEK067F3R_rc /REP_DB=TREMBL Accession /GB=D71349 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk67f3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182192(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182192 NCBI Caenorhabditis elegans Argonaute (plant)-Like Gene family member (alg-2) (alg-2) mRNA, complete cds. 10/11 None
T07D3.7a ENSEMBL cdna:known chromosome:CEL140:II:873182:878951:1 gene:T07D3.7 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173468 2 1 NM_182192 174804_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:878538-878936(+) 100.0 100.0
Public Domain and Genome References
Ensembl T07D3.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66187 Argonaute (plant)-like gene protein 2, isoform a [Caenorhabditis elegans] ref|NP_871992.1| Argonaute (plant)-Like Gene family member (alg-2) [Caenorhabditis elegans] 0.0
blast AAO38604 Argonaute (plant)-like gene protein 2, isoform b [Caenorhabditis elegans] ref|NP_493837.1| Argonaute (plant)-Like Gene family member (alg-2) [Caenorhabditis elegans] pir||T32079 hypothetical protein T07D3.7 - Caenorhabditis elegans 0.0
blast CAE63062 Hypothetical protein CBG07340 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 6.8E-55
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174804_AT
gccccctgagattttcatgtttttccttcccctaattatcaatatcaatcaatactttaa
tatctttcaagcccaggtttataatatttccaattatgttaggtgtttaattttcaccaa
gttttttttccatttttttccaaatttcttgagccattttccatactagccacacaatgc
tttttcgatcctccaaatactttccatttgtgtctttatcccctctgctctaattaaata
cgaccttatcactcaattggtacactttgttgtaaactgtctttctcttagtatctgaaa
aaaaacctcatgattgtcctgtttttcaaccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCCCCTGAGATTTTCATGTTTTTC 32 287 37 Antisense
TTTTCCTTCCCCTAATTATCAATAT 225 677 57 Antisense
ATATCTTTCAAGCCCAGGTTTATAA 154 17 96 Antisense
TGAGCCATTTTCCATACTAGCCACA 646 569 186 Antisense
ACTAGCCACACAATGCTTTTTCGAT 376 101 201 Antisense
TACTTTCCATTTGTGTCTTTATCCC 557 643 234 Antisense
ATCCCCTCTGCTCTAATTAAATACG 249 39 254 Antisense
AAATACGACCTTATCACTCAATTGG 188 117 272 Antisense
CACTTTGTTGTAAACTGTCTTTCTC 355 197 299 Antisense
AACTGTCTTTCTCTTAGTATCTGAA 221 143 311 Antisense
CATGATTGTCCTGTTTTTCAACCCA 169 211 345 Antisense
Sequence Source Affymetrix Proprietary Database