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[174796_at] fatty acid synthase

Description CEK013C7R_rc /REP_DB=TREMBL Accession /GB=D27368 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13c7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 03      6grp_total > 15      4grp_total > 59      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3858
1 2 3 4 range
A 2734.5
(-0.3)
P 		3302
(1)
P 		4193
(3)
P 		1512.6
(-3)
P 		2680
B 2730.5
(-0.1)
P 		4505.2
(3)
P 		3035.7
(0.4)
P 		1120.1
(-3)
P 		3385
C 2692.9
(-0.8)
P 		4978.1
(3)
P 		4278.9
(1.8)
P 		1353.2
(-3)
P 		3625
avr 2719.3
(-0.2) 		4261.8
(3) 		3835.9
(2.1) 		1328.6
(-3) 		2933

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4816
1 2 3 4 5 6 range
A 5989.8
(3)
P 		4111.8
(0.5)
P 		2442.3
(-1.7)
P 		1722.5
(-2.7)
P 		2306.3
(-1.9)
P 		1474.4
(-3)
P 		4515
B 4948.1
(3)
P 		4245.4
(1.9)
P 		3814.6
(1.2)
P 		3348.1
(0.5)
P 		1173.8
(-3)
P 		2034.4
(-1.6)
P 		3774
C 5189.3
(3)
P 		4110.6
(1.3)
P 		2735.2
(-1)
P 		2084.8
(-2)
P 		1462.1
(-3)
P 		2053.7
(-2)
P 		3727
avr 5375.7
(3) 		4155.9
(1) 		2997.4
(-0.8) 		2385.1
(-1.8) 		1647.4
(-3) 		1854.2
(-2.7) 		3728
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1270
1 2 3 4 range
A 995.6
(2.3)
P 		716.8
(0.4)
P 		1096.1
(3)
P 		221.4
(-3)
P 		875
B 870.7
(1.3)
P 		1112.7
(3)
P 		703.4
(0.2)
P 		238.7
(-3)
P 		874
C 891.4
(0.2)
P 		1431.2
(2.7)
P 		1491.3
(3)
P 		228.2
(-3)
P 		1263
avr 919.2
(1.8) 		1086.9
(2.9) 		1096.9
(3) 		229.4
(-3) 		868

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1564
1 2 3 4 5 6 range
A 1104.2
(1.1)
P 		1462.3
(3)
P 		531.9
(-2.1)
P 		436.9
(-2.6)
P 		696
(-1.2)
P 		359
(-3)
P 		1103
B 1721.7
(3)
P 		1413.1
(1.5)
P 		926.3
(-0.8)
P 		886.8
(-1)
P 		476.9
(-3)
P 		758.6
(-1.6)
P 		1245
C 1923.4
(3)
P 		1254.3
(0.3)
P 		696.2
(-1.9)
P 		419.3
(-3)
P 		473.1
(-2.8)
P 		687.5
(-1.9)
P 		1504
avr 1583.1
(3) 		1376.6
(1.8) 		718.1
(-2) 		581
(-2.8) 		548.7
(-3) 		601.7
(-2.7) 		1034
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174796_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20400
Sequence Type Exemplar sequence
Representative Public ID CEK013C7R NCBI
Target Description CEK013C7R_rc /REP_DB=TREMBL Accession /GB=D27368 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13c7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060016(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060016 NCBI Caenorhabditis elegans F32H2.5 (F32H2.5) mRNA, complete cds. 11/11 None
F32H2.5 ENSEMBL cdna:known chromosome:CEL140:I:8983508:8992895:-1 gene:F32H2.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8983452-8983861(-) 100.0 100.0
Public Domain and Genome References
Gene Title fatty acid synthase
Ensembl F32H2.5
Entrez Gene 172715 Entrez gene
SwissProt P91871 EMBL-EBI
WormBase CE09880 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17136.1.S1_AT similar to fatty acid synthase cfa
CANINE_2:CFA.17678.1.S1_S_AT similar to fatty acid synthase cfa
CANINE_2:CFAAFFX.10018.1.S1_S_AT similar to fatty acid synthase cfa
CANINE_2:CFAAFFX.10039.1.S1_S_AT similar to fatty acid synthase cfa
DROSOPHILA_2:1624549_AT dm
DROSGENOME1:152126_AT dm
CHICKEN:GGA.2448.1.S2_AT fatty acid synthase gga
HU35KSUBC:RC_AA058907_F_AT fatty acid synthase hs
HUGENEFL:S80437_S_AT fatty acid synthase hs
U133_X3P:HS.83190.1.S1_3P_A_AT fatty acid synthase hs
U133_X3P:HS.11050.2.A2_3P_S_AT fatty acid synthase hs
HG-U133A_2:212218_S_AT fatty acid synthase hs
HG-FOCUS:212218_S_AT fatty acid synthase hs
HG-U133_PLUS_2:212218_S_AT fatty acid synthase hs
HG-U133A:212218_S_AT fatty acid synthase hs
HG-U95AV2:38429_AT fatty acid synthase hs
MOE430A:1423828_AT fatty acid synthase mm
MOUSE430A_2:1423828_AT fatty acid synthase mm
MOUSE430_2:1423828_AT fatty acid synthase mm
MU11KSUBB:X13135_S_AT fatty acid synthase mm
MG-U74AV2:98575_AT fatty acid synthase mm
MG-U74BV2:110645_AT fatty acid synthase mm
MG-U74BV2:110644_S_AT fatty acid synthase mm
MU19KSUBB:TC33017_AT Fatty acid synthase, mRNA (cDNA clone IMAGE:5699125) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6633 fatty acid biosynthesis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4024 alcohol dehydrogenase activity, zinc-dependent inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
16740 transferase activity inferred from electronic annotation QuickGO AmiGO
16788 hydrolase activity, acting on ester bonds inferred from electronic annotation QuickGO AmiGO
48037 cofactor binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04244 Hypothetical protein F32H2.5 [Caenorhabditis elegans] ref|NP_492417.1| F32H2.5 [Caenorhabditis elegans] 0.0
blast CAE67008 Hypothetical protein CBG12408 [Caenorhabditis briggsae] 0.0
blast P12276 Fatty acid synthase [Includes: [Acyl-carrier-protein] S-acetyltransferase ; [Acyl-carrier-protein] S-malonyltransferase ; 3-oxoacyl-[acyl-carrier-protein] synthase ; 3-oxoacyl-[acyl-carrier-protein] reductase ; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase ; Enoyl-[acyl-carrier-protein] reductase ; Oleoyl-[acyl-carrier-protein] hydrolase ] gb|AAB46389.1| fatty acid synthase 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001227 EMBL-EBI Acyl transferase domain 1.5E-64
Pfam IPR001031 EMBL-EBI Thioesterase 3.5E-58
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 7.6E-94
Pfam IPR002085 EMBL-EBI Zinc-containing alcohol dehydrogenase superfamily 5.0E-62
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.3E-74
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.2E-7
Sequence
Target Sequence 
>C. ELEGANS:174796_AT
ctctttgctcaagactcatgagtgtgtgtattagttttcaatggattagttagttttcta
atgttttctaggactgcccacccttgatttcaattgaattctaatcattttccatatgtt
tagtaccatgctttcttctctctttgctcgatctgtctgtacttgaccccctttcccccc
tcttcacaggtctctctctctcccccagtgccatgccgttacggttacgtaggttactga
ttctttcccattccttcttctcgaaacgtttctttttgttttaaattttcttttataact
caattttcacgcggaaccaattgtttttaatgtgtttcattttgtaacttttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTTTGCTCAAGACTCATGAGTGT 175 231 16 Antisense
GAGTGTGTGTATTAGTTTTCAATGG 569 401 35 Antisense
TCTAATGTTTTCTAGGACTGCCCAC 174 611 72 Antisense
CTGCCCACCCTTGATTTCAATTGAA 215 235 89 Antisense
GAATTCTAATCATTTTCCATATGTT 663 323 111 Antisense
TGTTTAGTACCATGCTTTCTTCTCT 297 561 132 Antisense
TGCTCGATCTGTCTGTACTTGACCC 400 581 160 Antisense
AGTGCCATGCCGTTACGGTTACGTA 183 61 222 Antisense
GTAGGTTACTGATTCTTTCCCATTC 608 455 244 Antisense
AACTCAATTTTCACGCGGAACCAAT 19 3 312 Antisense
ATGTGTTTCATTTTGTAACTTTTCA 643 49 345 Antisense
Sequence Source Affymetrix Proprietary Database