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[174795_at]

Description CEK069E6R_rc /REP_DB=TREMBL Accession /GB=D65838 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk69e6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 01      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 18      6grp_total > 33      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > De      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8448
1 2 3 4 range
A 16440.3
(3)
P 		13350.3
(0.1)
P 		16435.2
(3)
P 		10137.9
(-3)
P 		6302
B 16563.5
(1.5)
P 		16455.4
(1.4)
P 		18586
(3)
P 		10685.4
(-3)
P 		7901
C 15487
(0.6)
P 		18385.8
(3)
P 		15920.8
(1)
P 		11041.7
(-3)
P 		7344
avr 16163.6
(2.2) 		16063.8
(2.1) 		16980.7
(3) 		10621.7
(-3) 		6359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6897
1 2 3 4 5 6 range
A 18514.1
(3)
P 		15686.6
(-0.7)
P 		18141.1
(2.5)
P 		13951.5
(-3)
P 		16967.9
(1)
P 		14817.3
(-1.9)
P 		4563
B 17689.4
(3)
P 		13847.3
(-0.8)
P 		15299.8
(0.6)
P 		15879.3
(1.2)
P 		13879.6
(-0.8)
P 		11617.6
(-3)
P 		6072
C 14249.2
(0)
P 		14005.5
(-0.4)
P 		15539.3
(2.4)
P 		15101.4
(1.6)
P 		15893.9
(3)
P 		12576.3
(-3)
P 		3318
avr 16817.6
(3) 		14513.1
(-0.6) 		16326.7
(2.2) 		14977.4
(0.1) 		15580.5
(1.1) 		13003.7
(-3) 		3814
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4502
1 2 3 4 range
A 5985.9
(3)
P 		2898
(-1.1)
P 		4296.5
(0.7)
P 		1484
(-3)
P 		4502
B 5282
(3)
P 		4064.1
(0.6)
P 		4306.8
(1.1)
P 		2277.1
(-3)
P 		3005
C 5126.6
(2.3)
P 		5286
(2.6)
P 		5548.9
(3)
P 		1861.8
(-3)
P 		3687
avr 5464.8
(3) 		4082.7
(0.7) 		4717.4
(1.8) 		1874.3
(-3) 		3591

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3118
1 2 3 4 5 6 range
A 3412.9
(-3)
P 		5578.9
(3)
P 		3950.7
(-1.5)
P 		3538.4
(-2.7)
P 		5120.2
(1.7)
P 		3607.5
(-2.5)
P 		2166
B 6155.1
(3)
P 		4609.2
(-0.8)
P 		3715.3
(-3)
P 		4205.9
(-1.8)
P 		5639.1
(1.7)
P 		4332.1
(-1.5)
P 		2440
C 5281.6
(3)
P 		4273.5
(0.3)
P 		3955
(-0.5)
P 		3036.9
(-3)
P 		5143
(2.6)
P 		4210
(0.1)
P 		2245
avr 4949.9
(1.8) 		4820.5
(1.3) 		3873.7
(-2) 		3593.7
(-3) 		5300.8
(3) 		4049.9
(-1.4) 		1707
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174795_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22082
Sequence Type Exemplar sequence
Representative Public ID CEK069E6R NCBI
Target Description CEK069E6R_rc /REP_DB=TREMBL Accession /GB=D65838 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk69e6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076440(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076440 NCBI Caenorhabditis elegans ACTin family member (act-4) (act-4) mRNA, complete cds. 11/11 None
M03F4.2a.1 ENSEMBL cdna:known chromosome:CEL140:X:4960520:4964383:1 gene:M03F4.2 11/11 A 
M03F4.2a.2 ENSEMBL cdna:known chromosome:CEL140:X:4960546:4964527:1 gene:M03F4.2 11/11 A 
M03F4.2a.3 ENSEMBL cdna:known chromosome:CEL140:X:4960546:4967028:1 gene:M03F4.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180767 3 1 NM_076440 174795_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4964040-4964366(+) 100.0 100.0
Public Domain and Genome References
Ensembl M03F4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR21857 actin [Cooperia oncophora] gb|AAB04575.1| Actin protein 4, isoform a [Caenorhabditis elegans] emb|CAA34720.1| actin [Caenorhabditis elegans] ref|NP_508841.1| ACTin family member (act-4) [Caenorhabditis elegans] gb|AAY52453.1| actin [Caenorhabditis remanei] sp|P10986|ACT4_CAEEL Actin-4 emb|CAE68670.1| Hypothetical protein CBG14574 [Caenorhabditis briggsae] emb|CAE75153.1| Hypothetical protein CBG23090 [Caenorhabditis briggsae] 0.0
blast CAB04678 Hypothetical protein T04C12.6 [Caenorhabditis elegans] emb|CAB04676.1| Hypothetical protein T04C12.4 [Caenorhabditis elegans] ref|NP_505819.1| T04C12.6 [Caenorhabditis elegans] ref|NP_505817.1| T04C12.4 [Caenorhabditis elegans] sp|P10983|ACT1_CAEEL Actin-1/3 emb|CAA34717.1| actin [Caenorhabditis elegans] 0.0
blast AAQ89578 actin [Panagrellus redivivus] gb|AAM47606.1| actin [Panagrellus redivivus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174795_AT
ccctctccgtgtacattgctagtaatagttccaacttctctagccgactgcattatgctt
cccattctcacccaaatcttcattggtaacttctcttgtatttgttcaatactattccgg
tagtaatctaggcatgtataactaggttctctgtgtatctttggtttgaagttgaggttt
aatgcaatcacttttgttgttgtaccctattactgccgatgacccccattttctcattac
aaaattgacattttaaaccattttttctctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCTCTCCGTGTACATTGCTAGTAA 396 259 33 Antisense
GTAATAGTTCCAACTTCTCTAGCCG 623 461 54 Antisense
CTAGCCGACTGCATTATGCTTCCCA 18 225 72 Antisense
AATCTTCATTGGTAACTTCTCTTGT 35 171 107 Antisense
TATTTGTTCAATACTATTCCGGTAG 156 663 131 Antisense
ATTCCGGTAGTAATCTAGGCATGTA 163 9 146 Antisense
GTATAACTAGGTTCTCTGTGTATCT 424 453 168 Antisense
GCAATCACTTTTGTTGTTGTACCCT 569 319 216 Antisense
TTTGTTGTTGTACCCTATTACTGCC 404 665 225 Antisense
GACCCCCATTTTCTCATTACAAAAT 498 347 253 Antisense
GACATTTTAAACCATTTTTTCTCTG 161 371 279 Antisense
Sequence Source Affymetrix Proprietary Database