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[174784_at]

Description CEK046A8R_rc /REP_DB=TREMBL Accession /GB=D34446 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk46a8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 24      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 61      6grp_total > 03      4grp_total > 55      6grp_cgc > 41      6grp_high1_sub > 02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 588
1 2 3 4 range
A 648.8
(3)
P 		296.5
(-1.5)
P 		341.7
(-0.9)
P 		179.3
(-3)
P 		470
B 767.6
(3)
P 		375.2
(-1.8)
P 		303.7
(-2.7)
P 		275.5
(-3)
P 		492
C 585
(3)
P 		357.3
(-1.5)
P 		340.2
(-1.8)
P 		280.6
(-3)
P 		304
avr 667.1
(3) 		343
(-1.6) 		328.5
(-1.8) 		245.1
(-3) 		422

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 246.3
(-3)
P 		441.5
(3)
P 		277.7
(-2)
P 		357.9
(0.4)
P 		381.3
(1.1)
P 		359.7
(0.5)
P 		195
B 490.3
(3)
P 		387
(0.4)
P 		274.3
(-2.5)
P 		402.3
(0.7)
P 		392.6
(0.5)
P 		256.4
(-3)
P 		234
C 558.1
(3)
P 		515
(2.1)
P 		319
(-2.3)
P 		369.4
(-1.2)
P 		342
(-1.8)
P 		285.8
(-3)
P 		272
avr 431.6
(2.4) 		447.8
(3) 		290.3
(-3) 		376.5
(0.3) 		372
(0.1) 		300.6
(-2.6) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 236.2
(3)
P 		64.4
(-1.9)
P 		89.3
(-1.2)
P 		26.3
(-3)
P 		210
B 244.8
(3)
P 		92.7
(-1.9)
P 		70.4
(-2.6)
P 		58.7
(-3)
P 		186
C 193.7
(3)
P 		102.7
(-0.7)
P 		118.6
(-0.1)
P 		47.3
(-3)
P 		146
avr 224.9
(3) 		86.6
(-1.6) 		92.8
(-1.4) 		44.1
(-3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 45.4
(-3)
P 		157
(3)
P 		60.5
(-2.2)
P 		90.8
(-0.6)
P 		115
(0.7)
P 		87.6
(-0.7)
P 		112
B 170.6
(3)
P 		128.8
(0.6)
P 		66.6
(-3)
P 		106.6
(-0.7)
P 		159.5
(2.4)
P 		95.6
(-1.3)
P 		104
C 206.9
(3)
P 		157.1
(0.7)
P 		81.2
(-2.7)
P 		74.3
(-3)
P 		110.7
(-1.4)
P 		95.7
(-2)
P 		133
avr 141
(2.5) 		147.6
(3) 		69.4
(-3) 		90.6
(-1.4) 		128.4
(1.5) 		93
(-1.2) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22458
Sequence Type Exemplar sequence
Representative Public ID CEK046A8R NCBI
Target Description CEK046A8R_rc /REP_DB=TREMBL Accession /GB=D34446 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk46a8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076209(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076209 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-7) (rgs-7) mRNA, complete cds. 11/11 A 
F56B6.2a ENSEMBL cdna:known chromosome:CEL140:X:3542545:3557582:1 gene:F56B6.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180645 3 1 NM_076209 174784_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3557152-3557550(+) 100.0 100.0
Public Domain and Genome References
Ensembl F56B6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAS75452 RGS-7A [Caenorhabditis elegans] gb|AAL11462.1| Regulator of g protein signaling protein 7, isoform a [Caenorhabditis elegans] ref|NP_508610.3| Regulator of G protein Signaling family member (rgs-7) [Caenorhabditis elegans] sp|Q8WQC0|RGS7_CAEEL Regulator of G-protein signaling rgs-7 (Regulator of G-protein signaling c2) (Protein C2-RGS) 0.0
blast AAL65791 Regulator of g protein signaling protein 7, isoform b [Caenorhabditis elegans] ref|NP_001024714.1| Regulator of G protein Signaling family member (rgs-7) [Caenorhabditis elegans] dbj|BAB85494.1| C2-RGS protein [Caenorhabditis elegans] 0.0
blast CAE68445 Hypothetical protein CBG14233 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-6
Pfam IPR000342 EMBL-EBI Regulator of G protein 3.6E-40
Sequence
Target Sequence 
>C. ELEGANS:174784_AT
tcattttatgatctttttgttttcccacccccgcacaatataaattgaaggtagtgcaaa
tgtgtcctctttttctctctctcacgtagttgtcattttcattggaactttatctgtacg
tttctattctttgaaacctgtctaaaatttcaacatgccgctcatttcgttgagcttttt
tctcatcttttctgtattctaattgaatcagactcttaattgccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATTTTATGATCTTTTTGTTTTCC 372 619 21 Antisense
CCCACCCCCGCACAATATAAATTGA 93 259 44 Antisense
GTAGTGCAAATGTGTCCTCTTTTTC 57 457 71 Antisense
CTCTCTCACGTAGTTGTCATTTTCA 565 229 97 Antisense
GTCATTTTCATTGGAACTTTATCTG 457 467 112 Antisense
ACTTTATCTGTACGTTTCTATTCTT 331 103 127 Antisense
TTCTATTCTTTGAAACCTGTCTAAA 278 691 142 Antisense
AATTTCAACATGCCGCTCATTTCGT 541 173 166 Antisense
ATGCCGCTCATTTCGTTGAGCTTTT 630 41 175 Antisense
TTTTCTCATCTTTTCTGTATTCTAA 345 677 198 Antisense
ATTGAATCAGACTCTTAATTGCCAG 414 5 222 Antisense
Sequence Source Affymetrix Proprietary Database