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[174766_at]

Description CEK028G8R_rc /REP_DB=TREMBL Accession /GB=D33294 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk28g8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 04      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 11      6grp_total > 15      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2001
1 2 3 4 range
A 2610.7
(3)
P 		1877
(0.3)
P 		1244.8
(-2)
P 		981.8
(-3)
P 		1629
B 2673.5
(3)
P 		2032.1
(0.6)
P 		1265.5
(-2.3)
P 		1070.7
(-3)
P 		1603
C 2982.7
(3)
P 		1900.4
(-0.5)
P 		1345.6
(-2.2)
P 		1105.3
(-3)
P 		1877
avr 2755.6
(3) 		1936.5
(0.1) 		1285.3
(-2.2) 		1052.6
(-3) 		1703

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1758
1 2 3 4 5 6 range
A 1985.5
(3)
P 		1934.8
(2.8)
P 		1231.1
(-0.3)
P 		931.1
(-1.7)
P 		1004.6
(-1.3)
P 		628.5
(-3)
P 		1357
B 2386.3
(3)
P 		2036.7
(1.7)
P 		1646.3
(0.3)
P 		1030.1
(-2)
P 		1146.1
(-1.6)
P 		768.7
(-3)
P 		1618
C 2220.9
(3)
P 		2019.2
(2.2)
P 		1266.1
(-0.8)
P 		808.7
(-2.6)
P 		1146.2
(-1.3)
P 		715.4
(-3)
P 		1506
avr 2197.6
(3) 		1996.9
(2.2) 		1381.2
(-0.3) 		923.3
(-2.1) 		1099
(-1.4) 		704.2
(-3) 		1493
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 844
1 2 3 4 range
A 950.5
(3)
P 		407.4
(-1)
P 		325.4
(-1.6)
P 		143.7
(-3)
P 		807
B 852.5
(3)
P 		501.9
(-0.4)
P 		293.2
(-2.4)
P 		228.2
(-3)
P 		624
C 987.4
(3)
P 		546.4
(-0.3)
P 		469
(-0.9)
P 		186.4
(-3)
P 		801
avr 930.1
(3) 		485.2
(-0.6) 		362.5
(-1.6) 		186.1
(-3) 		744

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 677
1 2 3 4 5 6 range
A 366
(-0.6)
P 		688.1
(3)
P 		268.1
(-1.7)
P 		236.1
(-2.1)
P 		303.1
(-1.3)
P 		153
(-3)
P 		535
B 830.3
(3)
P 		677.9
(1.4)
P 		399.8
(-1.6)
P 		272.8
(-3)
P 		465.6
(-0.9)
P 		286.7
(-2.9)
P 		558
C 823.2
(3)
P 		616.1
(1.1)
P 		322.2
(-1.6)
P 		162.6
(-3)
P 		370.9
(-1.1)
P 		239.5
(-2.3)
P 		661
avr 673.2
(3) 		660.7
(2.8) 		330
(-1.6) 		223.8
(-3) 		379.9
(-0.9) 		226.4
(-3) 		449
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174766_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20304
Sequence Type Exemplar sequence
Representative Public ID CEK028G8R NCBI
Target Description CEK028G8R_rc /REP_DB=TREMBL Accession /GB=D33294 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk28g8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026520(11), NM_001026521(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026520 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-18) (eat-18) mRNA, complete cds. 11/11 None
NM_001026521 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-18) (eat-18) mRNA, complete cds. 11/11 None
SNAP00000013327 ENSEMBL cdna:SNAP chromosome:CEL140:I:14404471:14408284:1 10/11 None
Y105E8A.7a ENSEMBL cdna:known chromosome:CEL140:I:14398451:14408340:1 gene:Y105E8A.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173302 4 2 NM_001026520 174766_at (11), 176962_s_at (11)
NM_001026521 174766_at (11), 176961_at (9), 176962_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14407688-14408250(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y105E8A.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21658 Hypothetical protein Y105E8A.7a [Caenorhabditis elegans] ref|NP_001021691.1| EATing: abnormal pharyngeal pumping family member (eat-18) [Caenorhabditis elegans] tpe|CAE82298.1| TPA: LEV-10A protein [Caenorhabditis elegans] 0.0
blast CAE64130 Hypothetical protein CBG08746 [Caenorhabditis briggsae] 0.0
blast CAF31497 Hypothetical protein Y105E8A.7b [Caenorhabditis elegans] ref|NP_001021692.1| EATing: abnormal pharyngeal pumping family member (eat-18) [Caenorhabditis elegans] tpe|CAE82299.1| TPA: LEV-10B [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000859 EMBL-EBI CUB 2.3E-10
Pfam IPR000859 EMBL-EBI CUB 3.9E-19
Pfam IPR000859 EMBL-EBI CUB 1.1E-6
Pfam IPR000859 EMBL-EBI CUB 9.3E-19
Pfam IPR000859 EMBL-EBI CUB 4.3E-13
Pfam IPR000859 EMBL-EBI CUB 1.1E-6
Pfam IPR000859 EMBL-EBI CUB 9.3E-19
Pfam IPR000859 EMBL-EBI CUB 4.3E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021691.1 1 810-832
NP_001021692.1 1 817-839
Y105E8A.7a 1 810-832
SNAP00000013327 1 519-541
Sequence
Target Sequence 
>C. ELEGANS:174766_AT
gattcttcagaattatcgccaccggccaccagtcgattcgttcatcatgacgcaacggga
atgatgccaccaattcagttacatcagattgggagttcatcgcgtgatatgtatgcttag
tagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTTCAGAATTATCGCCACCGG 556 431 202 Antisense
CCACCAGTCGATTCGTTCATCATGA 261 273 227 Antisense
GTCGATTCGTTCATCATGACGCAAC 476 473 233 Antisense
GATTCGTTCATCATGACGCAACGGG 234 433 236 Antisense
GACGCAACGGGAATGATGCCACCAA 164 381 250 Antisense
GATGCCACCAATTCAGTTACATCAG 335 415 264 Antisense
GCCACCAATTCAGTTACATCAGATT 140 279 267 Antisense
ACATCAGATTGGGAGTTCATCGCGT 515 105 282 Antisense
GATTGGGAGTTCATCGCGTGATATG 271 435 288 Antisense
GAGTTCATCGCGTGATATGTATGCT 38 399 294 Antisense
CGCGTGATATGTATGCTTAGTAGTG 709 253 302 Antisense
Sequence Source Affymetrix Proprietary Database