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[174746_at]

Description CEK041D9R_rc /REP_DB=TREMBL Accession /GB=D34130 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41d9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 56      6grp_total > 19      4grp_total > 60      6grp_cgc > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1117
1 2 3 4 range
A 2144.5
(1.8)
P 		1873.9
(-0.1)
P 		2314.5
(3)
P 		1464
(-3)
P 		851
B 2278.6
(0.5)
P 		2236
(0.2)
P 		2580.8
(3)
P 		1846.6
(-3)
P 		734
C 2108.2
(0.2)
P 		2133.6
(0.4)
P 		2503.5
(3)
P 		1665.8
(-3)
P 		838
avr 2177.1
(0.9) 		2081.2
(0.1) 		2466.3
(3) 		1658.8
(-3) 		808

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1249
1 2 3 4 5 6 range
A 2508.2
(3)
P 		2212
(1.2)
P 		1858.7
(-0.9)
P 		1617.5
(-2.4)
P 		2345.5
(2)
P 		1514.8
(-3)
P 		993
B 2279.5
(3)
P 		2224.7
(2.4)
P 		1734.4
(-3)
P 		2278.9
(3)
P 		2064.3
(0.6)
P 		1989.4
(-0.2)
P 		545
C 2764.1
(3)
P 		1939
(-1)
P 		1889.2
(-1.2)
P 		1685.2
(-2.2)
P 		2278.3
(0.7)
P 		1516.9
(-3)
P 		1247
avr 2517.3
(3) 		2125.2
(0.2) 		1827.4
(-1.9) 		1860.5
(-1.7) 		2229.4
(1) 		1673.7
(-3) 		844
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 658
1 2 3 4 range
A 780.8
(3)
P 		406.8
(-1)
P 		605.1
(1.1)
P 		214.3
(-3)
P 		567
B 726.6
(3)
P 		552.2
(-0.1)
P 		598
(0.7)
P 		393.5
(-3)
P 		333
C 697.9
(1.2)
P 		613.4
(0.4)
P 		872.5
(3)
P 		280.9
(-3)
P 		592
avr 735.1
(3) 		524.1
(0.1) 		691.9
(2.4) 		296.2
(-3) 		439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 686
1 2 3 4 5 6 range
A 462.4
(-1.7)
P 		786.7
(3)
P 		404.8
(-2.5)
P 		410.2
(-2.4)
P 		707.8
(1.9)
P 		368.8
(-3)
P 		418
B 793.1
(2.3)
P 		740.5
(1.6)
P 		421.2
(-3)
P 		603.6
(-0.4)
P 		838.7
(3)
P 		741.9
(1.6)
P 		418
C 1024.5
(3)
P 		591.6
(-0.8)
P 		480.8
(-1.8)
P 		338.9
(-3)
P 		737.2
(0.5)
P 		507.8
(-1.5)
P 		686
avr 760
(3) 		706.3
(2) 		435.6
(-3) 		450.9
(-2.7) 		761.2
(3) 		539.5
(-1.1) 		326
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174746_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21719
Sequence Type Exemplar sequence
Representative Public ID CEK041D9R NCBI
Target Description CEK041D9R_rc /REP_DB=TREMBL Accession /GB=D34130 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41d9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029023(11), NM_001029022(10), NM_001029024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029023 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-1) (rme-1) mRNA, complete cds. 11/11 None
NM_001029022 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-1) (rme-1) mRNA, complete cds. 10/11 None
NM_001029024 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-1) (rme-1) mRNA, complete cds. 11/11 None
W06H8.1c ENSEMBL cdna:known chromosome:CEL140:V:6211434:6220242:1 gene:W06H8.1 11/11 A 
W06H8.1b ENSEMBL cdna:known chromosome:CEL140:V:6212455:6220168:1 gene:W06H8.1 10/11 A 
W06H8.1d ENSEMBL cdna:known chromosome:CEL140:V:6214501:6220216:1 gene:W06H8.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179010 6 3 NM_001029022 174746_at (10)
NM_001029023 174746_at (11)
NM_001029024 174746_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6219874-6220220(+) 100.0 100.0
Public Domain and Genome References
Ensembl W06H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39980 Receptor mediated endocytosis protein 1, isoform c [Caenorhabditis elegans] ref|NP_001024194.1| Receptor Mediated Endocytosis family member (rme-1) [Caenorhabditis elegans] gb|AAK27716.1| receptor-mediated endocytosis 1 isoform I [Caenorhabditis elegans] 0.0
blast AAF39978 Receptor mediated endocytosis protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024192.1| Receptor Mediated Endocytosis family member (rme-1) [Caenorhabditis elegans] 0.0
blast AAF39979 Receptor mediated endocytosis protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024193.1| Receptor Mediated Endocytosis family member (rme-1) [Caenorhabditis elegans] gb|AAK27715.1| receptor-mediated endocytosis 1 isoform II [Caenorhabditis elegans] 0.0
blast AAK73902 Receptor mediated endocytosis protein 1, isoform d [Caenorhabditis elegans] ref|NP_001024195.1| Receptor Mediated Endocytosis family member (rme-1) [Caenorhabditis elegans] gb|AAK27714.1| receptor-mediated endocytosis 1 isoform IV [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.9E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.9E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.9E-4
Sequence
Target Sequence 
>C. ELEGANS:174746_AT
tttaccgaaaatgtcctatctagttaatatgaatcaagcaaacgtgttttgtgatacgac
ccccttctctcaccttcccttccaaattttcactctacatttatagaatgctttgaatgc
ataattcacatcccatgttcatttccccctattatttttttttgttttttctccgaccct
tccatctaaaaaaaataatttattgcctctaatttcgcaaaaaaatatggtcgactgctt
catggtattcgagttgacttttgccttgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTACCGAAAATGTCCTATCTAGTT 173 673 47 Antisense
ATGTCCTATCTAGTTAATATGAATC 699 47 57 Antisense
GCAAACGTGTTTTGTGATACGACCC 66 319 84 Antisense
CCTTCCAAATTTTCACTCTACATTT 589 269 124 Antisense
TTCACTCTACATTTATAGAATGCTT 197 687 135 Antisense
GAATGCATAATTCACATCCCATGTT 240 331 161 Antisense
AATTCACATCCCATGTTCATTTCCC 7 177 169 Antisense
ATAATTTATTGCCTCTAATTTCGCA 171 25 241 Antisense
AAATATGGTCGACTGCTTCATGGTA 709 115 269 Antisense
CGACTGCTTCATGGTATTCGAGTTG 643 251 278 Antisense
ATTCGAGTTGACTTTTGCCTTGACC 202 9 293 Antisense
Sequence Source Affymetrix Proprietary Database