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[174744_s_at] DNAJ domain

Description CEC1363_rc /REP_DB=TREMBL Accession /GB=C11136 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk164e12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 42      4grp_high_8_8 > 40      6grp_highest_nonorm > 70      6grp_total > 07      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 279.1
(-0.3)
P 		284.4
(3)
P 		274.7
(-3)
P 		277.1
(-1.5)
P 		10
B 350.5
(3)
P 		292.1
(-0.9)
P 		261.2
(-3)
P 		278.2
(-1.9)
P 		89
C 339.1
(2.3)
P 		290.5
(-3)
P 		345.5
(3)
P 		292.9
(-2.7)
P 		55
avr 322.9
(3) 		289
(-2.1) 		293.8
(-1.3) 		282.7
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 317.6
(3)
P 		275.8
(1.3)
P 		256
(0.5)
P 		297.1
(2.2)
P 		171.9
(-3)
P 		231.6
(-0.5)
P 		146
B 370.7
(3)
P 		300.6
(0.5)
P 		208
(-2.7)
P 		227.8
(-2)
P 		260.1
(-0.9)
P 		199.2
(-3)
P 		172
C 430.5
(3)
P 		265.2
(-0.7)
P 		265.7
(-0.7)
P 		163.8
(-3)
P 		241.4
(-1.3)
P 		223.7
(-1.7)
P 		267
avr 372.9
(3) 		280.5
(-0.6) 		243.2
(-2) 		229.6
(-2.6) 		224.5
(-2.8) 		218.2
(-3) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 101.6
(3)
P 		61.7
(-0.9)
P 		71.8
(0.1)
P 		40.6
(-3)
P 		61
B 111.8
(3)
P 		72.2
(-1.5)
P 		60.5
(-2.9)
P 		59.3
(-3)
P 		53
C 112.3
(2.3)
P 		83.5
(-0.1)
P 		120.4
(3)
P 		49.4
(-3)
P 		71
avr 108.6
(3) 		72.5
(-0.7) 		84.2
(0.5) 		49.8
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 58.6
(-2.1)
P 		98.1
(3)
P 		55.8
(-2.5)
P 		75.4
(0.1)
P 		51.9
(-3)
P 		56.4
(-2.4)
P 		46
B 129
(3)
P 		100.1
(0.8)
P 		50.5
(-3)
P 		60.3
(-2.3)
P 		105.7
(1.2)
P 		74.3
(-1.2)
P 		79
C 159.6
(3)
P 		80.9
(-0.7)
P 		67.6
(-1.4)
P 		32.9
(-3)
P 		78.1
(-0.9)
P 		74.9
(-1)
P 		127
avr 115.7
(3) 		93
(0.7) 		58
(-2.8) 		56.2
(-3) 		78.6
(-0.7) 		68.5
(-1.8) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174744_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21070
Sequence Type Exemplar sequence
Representative Public ID CEC1363 NCBI
Target Description CEC1363_rc /REP_DB=TREMBL Accession /GB=C11136 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk164e12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065438(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065438 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-16) (dnj-16) mRNA, complete cds. 11/11 A 
SNAP00000044748 ENSEMBL cdna:SNAP chromosome:CEL140:III:4191533:4193129:1 11/11 None
GENEFINDER00000044760 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4191533:4195088:1 11/11 None
R74.4 ENSEMBL cdna:known chromosome:CEL140:III:4191533:4193129:1 gene:R74.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4191599-4192148(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ domain
Gene Symbol dnj-16
Ensembl R74.4
Entrez Gene 175540 Entrez gene
SwissProt Q22028 EMBL-EBI
WormBase CE01057 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264865_AT DNAJ heat shock protein, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85274 Hypothetical protein R74.4 [Caenorhabditis elegans] ref|NP_497839.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-16) [Caenorhabditis elegans] 0.0
blast CAE72865 Hypothetical protein CBG20164 [Caenorhabditis briggsae] 0.0
blast AAL60201 X-box binding protein processed isoform [Caenorhabditis elegans] 1.0E-116
blast XP_467717 putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] ref|XP_506962.1| PREDICTED P0516G10.24 gene product [Oryza sativa (japonica cultivar-group)] dbj|BAD15765.1| putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] dbj|BAD15722.1| putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] 2.0E-46
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.00000003908296E-24
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 8.99999968283527E-25
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.8E-36
Sequence
Target Sequence 
>C. ELEGANS:174744_S_AT
gaaaatggcgtccgaggcagaaatcaaatcagcgtatcgaaaattggctcttaaatacca
tccagatcgtaatccaaatgatgctcatgctcaggaagagttcaagaaagtttcaatcgc
atattcagttttatctgatccaaataagcgaagacagtacgatgttagtggtccatcaga
aaatcaattggactttgaaggatttgatgtctccgaaatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAATGGCGTCCGAGGCAGAAATC 60 353 20 Antisense
GAGGCAGAAATCAAATCAGCGTATC 495 405 33 Antisense
TCAGCGTATCGAAAATTGGCTCTTA 400 623 48 Antisense
TTGGCTCTTAAATACCATCCAGATC 260 703 63 Antisense
ATACCATCCAGATCGTAATCCAAAT 351 23 74 Antisense
ATCCAAATGATGCTCATGCTCAGGA 676 39 91 Antisense
GATGCTCATGCTCAGGAAGAGTTCA 515 413 99 Antisense
TCAATCGCATATTCAGTTTTATCTG 114 633 132 Antisense
AGACAGTACGATGTTAGTGGTCCAT 74 71 171 Antisense
GTTAGTGGTCCATCAGAAAATCAAT 211 447 183 Antisense
GAAGGATTTGATGTCTCCGAAATGG 237 335 216 Antisense
Sequence Source Affymetrix Proprietary Database