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[174743_s_at]

Description CEK011E7R_rc /REP_DB=TREMBL Accession /GB=D32351 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11e7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 82      6grp_total > 79      4grp_total > 55      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 324
1 2 3 4 range
A 458.1
(3)
P 		136.9
(-3)
P 		279.4
(-0.3)
P 		201.6
(-1.8)
P 		321
B 407.4
(3)
P 		240
(-3)
P 		259.8
(-2.3)
P 		276.2
(-1.7)
P 		167
C 460.7
(3)
P 		226.1
(-3)
P 		331.8
(-0.3)
P 		306.1
(-1)
P 		235
avr 442.1
(3) 		201
(-3) 		290.3
(-0.8) 		261.3
(-1.5) 		241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 309
1 2 3 4 5 6 range
A 279.8
(-1.3)
P 		393.9
(1.6)
P 		218.8
(-2.8)
P 		212.6
(-3)
P 		450.5
(3)
P 		377.3
(1.2)
P 		238
B 389.7
(0.2)
P 		363.8
(-0.4)
P 		253.3
(-3)
P 		290
(-2.1)
P 		294
(-2)
P 		507
(3)
P 		254
C 306.9
(-0.3)
P 		310.1
(-0.3)
P 		206.4
(-2.8)
P 		197.6
(-3)
P 		372
(1.2)
P 		444.1
(3)
P 		247
avr 325.5
(-0.2) 		355.9
(0.6) 		226.2
(-3) 		233.4
(-2.8) 		372.2
(1) 		442.8
(3) 		217
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 166.8
(3)
P 		29.7
(-3)
P 		73
(-1.1)
P 		29.5
(-3)
P 		137
B 129.9
(3)
P 		59.3
(-3)
P 		60.2
(-2.9)
P 		58.9
(-3)
P 		71
C 152.5
(3)
P 		65
(-2.2)
P 		115.6
(0.8)
P 		51.6
(-3)
P 		101
avr 149.7
(3) 		51.3
(-2.7) 		82.9
(-0.9) 		46.7
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 51.6
(-2.7)
P 		140.1
(3)
P 		47.7
(-3)
P 		53.9
(-2.6)
P 		135.9
(2.7)
P 		91.9
(-0.1)
P 		92
B 135.6
(0.5)
P 		121.1
(-0.2)
P 		61.5
(-3)
P 		76.8
(-2.3)
P 		119.4
(-0.3)
P 		189.1
(3)
P 		128
C 113.7
(1.1)
P 		94.6
(0)
P 		52.5
(-2.3)
P 		39.7
(-3)
P 		120.4
(1.4)
P 		148.7
(3)
P 		109
avr 100.3
(0.1) 		118.6
(1.3) 		53.9
(-3) 		56.8
(-2.8) 		125.2
(1.8) 		143.2
(3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174743_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20692
Sequence Type Exemplar sequence
Representative Public ID CEK011E7R NCBI
Target Description CEK011E7R_rc /REP_DB=TREMBL Accession /GB=D32351 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11e7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062755 NCBI Caenorhabditis elegans ZK1248.10 (ZK1248.10) mRNA, complete cds. 11/11 None
ZK1248.10 ENSEMBL cdna:known chromosome:CEL140:II:5823388:5827408:-1 gene:ZK1248.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041389 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5823787-5824334(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1248.10
Entrez Gene 173986 Entrez gene
SwissProt Q23419 EMBL-EBI
WormBase CE29155 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21845.1.S1_AT similar to ZK1248.10 cfa
CHICKEN:GGAAFFX.2074.1.S1_AT similar to mKIAA1055 protein gga
HG-U133_PLUS_2:215640_AT KIAA1055 protein hs
HG-U133A_2:215640_AT KIAA1055 protein hs
HG-U133A:215640_AT KIAA1055 protein hs
HU35KSUBD:RC_R26293_AT KIAA1055 protein hs
HU35KSUBD:RC_H28210_AT KIAA1055 protein hs
HU35KSUBD:RC_AA447707_S_AT KIAA1055 protein hs
U133_X3P:HS.306386.0.S1_3P_AT KIAA1055 protein hs
U133_X3P:HS.126084.0.A1_3P_A_AT KIAA1055 protein hs
HG-U133A_2:212796_S_AT KIAA1055 protein hs
HG-U133_PLUS_2:212796_S_AT KIAA1055 protein hs
HG-U133A:212796_S_AT KIAA1055 protein hs
HG-U95AV2:39400_AT KIAA1055 protein hs
HG-U95E:68481_AT KIAA1055 protein hs
HG-U133_PLUS_2:215951_AT KIAA1055 protein hs
HG-U133A_2:215951_AT KIAA1055 protein hs
HG-U133A:215951_AT KIAA1055 protein hs
HG-U133_PLUS_2:230377_S_AT KIAA1055 protein hs
HG-U133B:230377_S_AT KIAA1055 protein hs
HG-U95D:91796_AT KIAA1055 protein hs
U133_X3P:HS.126084.1.A1_3P_AT KIAA1055 protein hs
MG-U74BV2:113749_AT RIKEN cDNA 1810061M12 gene mm
MG-U74BV2:107521_AT RIKEN cDNA 1810061M12 gene mm
MG-U74CV2:167050_R_AT RIKEN cDNA 1810061M12 gene mm
MG-U74CV2:167019_I_AT RIKEN cDNA 1810061M12 gene mm
MOE430B:1428508_AT RIKEN cDNA 1810061M12 gene mm
MOUSE430_2:1428508_AT RIKEN cDNA 1810061M12 gene mm
MU19KSUBB:TC31995_AT RIKEN cDNA 1810061M12 gene mm
MOUSE430_2:1444493_AT RIKEN cDNA 1810061M12 gene, mRNA (cDNA clone IMAGE:4039019) mm
MOE430B:1444493_AT RIKEN cDNA 1810061M12 gene, mRNA (cDNA clone IMAGE:4039019) mm
MU19KSUBA:TC19188_AT RIKEN cDNA 1810061M12 gene, mRNA (cDNA clone IMAGE:4039019) mm
MU19KSUBB:TC30945_AT RIKEN cDNA 1810061M12 gene, mRNA (cDNA clone IMAGE:4039019) mm
RAE230A:1372748_AT similar to KIAA1055 protein (predicted) rn
RAT230_2:1372748_AT similar to KIAA1055 protein (predicted) rn
RG-U34A:RC_AA799507_AT similar to KIAA1055 protein (predicted) rn
RG-U34B:RC_AI059020_AT Similar to KIAA1055 protein (predicted) rn
RG-U34C:RC_AI228491_AT Similar to KIAA1055 protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC71091 Hypothetical protein ZK1248.10 [Caenorhabditis elegans] ref|NP_495156.1| ZK1248.10 [Caenorhabditis elegans] 0.0
blast CAE59197 Hypothetical protein CBG02508 [Caenorhabditis briggsae] 0.0
blast XP_693084 PREDICTED: similar to mKIAA1055 protein [Danio rerio] 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 1.3E-47
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.1E-21
Sequence
Target Sequence 
>C. ELEGANS:174743_S_AT
ggccactgtatgtaaatccgacacgaacattcttgtacatctaatctgtattttctttcg
atttctagcataattatgtcttcttcacacaactttttcactattattttcaaacttctg
tgattctcctttttccttatcaatttcttttacggtttacttcccccccttcaacccgtt
tacttgtgctccgtttttacacatgagactgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCACTGTATGTAAATCCGACACG 562 543 184 Antisense
AATCCGACACGAACATTCTTGTACA 89 169 198 Antisense
CGAACATTCTTGTACATCTAATCTG 450 253 207 Antisense
ACATCTAATCTGTATTTTCTTTCGA 57 107 220 Antisense
GTATTTTCTTTCGATTTCTAGCATA 574 453 231 Antisense
ATTTCTAGCATAATTATGTCTTCTT 149 17 244 Antisense
TTCAAACTTCTGTGATTCTCCTTTT 588 683 292 Antisense
TGATTCTCCTTTTTCCTTATCAATT 319 563 304 Antisense
TTTACTTGTGCTCCGTTTTTACACA 548 673 362 Antisense
GTGCTCCGTTTTTACACATGAGACT 172 479 369 Antisense
TCCGTTTTTACACATGAGACTGAAA 296 595 373 Antisense
Sequence Source Affymetrix Proprietary Database