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[174742_at]

Description CEK033C7R_rc /REP_DB=TREMBL Accession /GB=D33593 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk33c7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 18      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 47      6grp_total > 16      4grp_total > 39      6grp_cgc > 03      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1723
1 2 3 4 range
A 4818.5
(3)
P 		3939.5
(-0.7)
P 		4075.2
(-0.1)
P 		3395.7
(-3)
P 		1423
B 5118.6
(3)
P 		3940.5
(-1.5)
P 		3538.4
(-3)
P 		4502
(0.7)
P 		1580
C 3875
(3)
P 		3484.3
(-2.3)
P 		3432.5
(-3)
P 		3828.7
(2.4)
P 		443
avr 4604
(3) 		3788.1
(-2.3) 		3682
(-3) 		3908.8
(-1.5) 		922

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2373
1 2 3 4 5 6 range
A 5164.1
(3)
P 		4467.7
(1.2)
P 		2897.4
(-3)
P 		3146
(-2.3)
P 		4074.6
(0.1)
P 		4119.1
(0.2)
P 		2267
B 4043
(3)
P 		3937.4
(2.5)
P 		2791.3
(-3)
P 		3711.4
(1.4)
P 		3408.5
(-0)
P 		3252.5
(-0.8)
P 		1252
C 3991.7
(1.7)
P 		3848.6
(1)
P 		4229.5
(3)
P 		3442.8
(-1.2)
P 		3546.8
(-0.7)
P 		3108.4
(-3)
P 		1121
avr 4399.6
(3) 		4084.6
(1.3) 		3306.1
(-3) 		3433.4
(-2.3) 		3676.6
(-1) 		3493.3
(-2) 		1094
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1257
1 2 3 4 range
A 1754.4
(3)
P 		855.2
(-1.3)
P 		1065.3
(-0.3)
P 		497.1
(-3)
P 		1257
B 1632.3
(3)
P 		973.2
(-1.9)
P 		819.9
(-3)
P 		959.4
(-2)
P 		812
C 1282.7
(3)
P 		1001.8
(0.4)
P 		1196.3
(2.2)
P 		645.6
(-3)
P 		637
avr 1556.5
(3) 		943.4
(-1.3) 		1027.2
(-0.7) 		700.7
(-3) 		856

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 958
1 2 3 4 5 6 range
A 952
(-1)
P 		1588.9
(3)
P 		631
(-3)
P 		797.9
(-2)
P 		1229.5
(0.7)
P 		1002.9
(-0.7)
P 		958
B 1406.8
(3)
P 		1310.6
(2.2)
P 		677.8
(-3)
P 		983
(-0.5)
P 		1384.8
(2.8)
P 		1212.9
(1.4)
P 		729
C 1479.6
(3)
P 		1174.3
(0.7)
P 		1076.5
(-0.1)
P 		692.4
(-3)
P 		1147.7
(0.5)
P 		1040.6
(-0.3)
P 		787
avr 1279.5
(2.2) 		1357.9
(3) 		795.1
(-3) 		824.4
(-2.7) 		1254
(1.9) 		1085.5
(0.1) 		563
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174742_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20963
Sequence Type Exemplar sequence
Representative Public ID CEK033C7R NCBI
Target Description CEK033C7R_rc /REP_DB=TREMBL Accession /GB=D33593 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk33c7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025962(11), NM_001025963(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025962 NCBI Caenorhabditis elegans SR Protein Kinase family member (spk-1) (spk-1) mRNA, complete cds. 11/11 None
NM_001025963 NCBI Caenorhabditis elegans SR Protein Kinase family member (spk-1) (spk-1) mRNA, complete cds. 11/11 None
B0464.5b.2 ENSEMBL cdna:known chromosome:CEL140:III:9456459:9462883:1 gene:B0464.5 11/11 None
B0464.5b.1 ENSEMBL cdna:known chromosome:CEL140:III:9456510:9462855:1 gene:B0464.5 11/11 None
B0464.5c.1 ENSEMBL cdna:known chromosome:CEL140:III:9456538:9462869:1 gene:B0464.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
B0464.5c.2 2/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176328 3 2 NM_001025962 174742_at (11)
NM_001025963 174742_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9462427-9462869(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0464.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA79541 Hypothetical protein B0464.5b [Caenorhabditis elegans] ref|NP_001021133.1| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] gb|AAG36873.1| SR protein specfic kinase SPK-1 [Caenorhabditis elegans] 0.0
blast CAA79540 Hypothetical protein B0464.5a [Caenorhabditis elegans] ref|NP_499080.3| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] sp|Q03563|SPK1_CAEEL Serine/threonine-protein kinase spk-1 0.0
blast CAA79542 Hypothetical protein B0464.5c [Caenorhabditis elegans] ref|NP_001021134.1| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] 0.0
blast Q61IS6 Serine/threonine-protein kinase spk-1 emb|CAE65212.1| Hypothetical protein CBG10087 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-28
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Sequence
Target Sequence 
>C. ELEGANS:174742_AT
aacaatcatccgactacttcatctccccattctccctgtcactttctacatcgaatcacc
gcatcctttctcttttttaccaccgttcttcctgaagtgactgagcagtaagcacgttta
gcgccactctcgtgcgcgtctcgttgaagcgccttttgatctttagtgctactggtataa
tataattattttgtacgaacatcgtccttatcctctgcccaacaacatcatatgcttcta
tcagcctcaaacagtagatcatcttttccccccatttctttttcatttatattcctccgt
ctgttcacatacccaaactatttggcataaacctttctcaaatctttactgtgcttcatt
gcttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAATCATCCGACTACTTCATCTC 265 135 24 Antisense
GTCACTTTCTACATCGAATCACCGC 318 463 61 Antisense
TTTACCACCGTTCTTCCTGAAGTGA 244 673 99 Antisense
AGCAGTAAGCACGTTTAGCGCCACT 619 75 127 Antisense
TCTCGTGCGCGTCTCGTTGAAGCGC 145 611 151 Antisense
GCGCCTTTTGATCTTTAGTGCTACT 600 287 172 Antisense
TGTACGAACATCGTCCTTATCCTCT 546 559 215 Antisense
TCCTCTGCCCAACAACATCATATGC 607 591 234 Antisense
ATCATATGCTTCTATCAGCCTCAAA 112 31 250 Antisense
CGTCTGTTCACATACCCAAACTATT 452 245 321 Antisense
ATCTTTACTGTGCTTCATTGCTTCC 565 31 365 Antisense
Sequence Source Affymetrix Proprietary Database