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[174732_s_at] xanthine dehydrogenase

Description CEK012F5R_rc /REP_DB=TREMBL Accession /GB=D27391 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk12f5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_high_8_8 > 0      6grp_highest_nonorm > 72      6grp_total > 75      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 160.9
(-0.3)
P 		164.5
(-0.2)
M 		86.7
(-3)
P 		254.3
(3)
P 		168
B 187.2
(1.8)
P 		74.4
(-3)
A 		216.1
(3)
P 		201.7
(2.4)
P 		142
C 224.2
(0.7)
P 		139
(-3)
P 		278.1
(3)
P 		212.6
(0.2)
P 		139
avr 190.8
(1) 		126
(-3) 		193.6
(1.2) 		222.9
(3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 368.5
(-1.9)
P 		341.1
(-3)
P 		410.4
(-0.1)
P 		450.5
(1.6)
P 		484.9
(3)
P 		401.9
(-0.5)
P 		144
B 363.5
(-1.3)
P 		298.5
(-3)
P 		308.1
(-2.7)
P 		475.5
(1.8)
P 		333.1
(-2.1)
P 		521.9
(3)
P 		223
C 347.2
(-2.2)
P 		361
(-1.9)
P 		553
(3)
P 		437.5
(0.1)
P 		317.4
(-3)
P 		467.9
(0.8)
P 		236
avr 359.7
(-1.8) 		333.5
(-3) 		423.8
(1.2) 		454.5
(2.6) 		378.5
(-0.9) 		463.9
(3) 		130
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 58.6
(3)
P 		35.7
(-0.8)
M 		22.7
(-3)
P 		37.2
(-0.6)
P 		36
B 59.7
(3)
P 		18.4
(-3)
A 		50.1
(1.6)
P 		43
(0.6)
P 		41
C 74.2
(0.8)
P 		40
(-2.6)
P 		96.9
(3)
P 		35.9
(-3)
P 		61
avr 64.2
(3) 		31.4
(-3) 		56.6
(1.6) 		38.7
(-1.7) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 67.9
(-3)
P 		121.3
(1.1)
P 		89.4
(-1.4)
P 		114.3
(0.6)
P 		146.3
(3)
P 		97.8
(-0.7)
P 		78
B 126.5
(-0.4)
P 		99.4
(-1.8)
P 		74.8
(-3)
P 		126
(-0.4)
P 		135.3
(0)
P 		194.6
(3)
P 		120
C 128.7
(0.6)
P 		110.1
(-1.1)
P 		140.7
(1.6)
P 		88
(-3)
P 		102.7
(-1.7)
P 		156.6
(3)
P 		69
avr 107.7
(-2.2) 		110.3
(-1.9) 		101.6
(-3) 		109.4
(-2) 		128.1
(0.3) 		149.7
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174732_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21788
Sequence Type Exemplar sequence
Representative Public ID CEK012F5R NCBI
Target Description CEK012F5R_rc /REP_DB=TREMBL Accession /GB=D27391 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk12f5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072976(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072976 NCBI Caenorhabditis elegans B0222.9 (B0222.9) mRNA, complete cds. 11/11 None
SNAP00000040461 ENSEMBL cdna:SNAP chromosome:CEL140:V:9171029:9175586:-1 11/11 None
GENEFINDER00000040468 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9171029:9175586:-1 11/11 None
B0222.9 ENSEMBL cdna:known chromosome:CEL140:V:9170987:9175586:-1 gene:B0222.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9170997-9171444(-) 100.0 100.0
Public Domain and Genome References
Gene Title xanthine dehydrogenase
Ensembl B0222.9
Entrez Gene 179299 Entrez gene
SwissProt Q960A1 EMBL-EBI
WormBase CE32787 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK71353 Hypothetical protein B0222.9 [Caenorhabditis elegans] ref|NP_505377.2| B0222.9 [Caenorhabditis elegans] 0.0
blast NP_500531 F15E6.6 [Caenorhabditis elegans] gb|AAB92058.2| Hypothetical protein F15E6.6 [Caenorhabditis elegans] 0.0
blast CAE72122 Hypothetical protein CBG19218 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008274 EMBL-EBI Aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding 1.0E-126
Pfam IPR002346 EMBL-EBI Molybdopterin dehydrogenase, FAD-binding 9.8E-4
Pfam IPR000674 EMBL-EBI Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead 8.9E-31
Pfam IPR002888 EMBL-EBI [2Fe-2S]-binding 1.7E-57
Pfam IPR001041 EMBL-EBI Ferredoxin 1.5E-11
Pfam IPR008274 EMBL-EBI Aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding 1.0E-126
Pfam IPR000674 EMBL-EBI Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead 5.2E-55
Sequence
Target Sequence 
>C. ELEGANS:174732_S_AT
tgtcatggatgtcggggaaagcttgaatccggccctcgatattggacaaattgaaggagc
attcatacaaggatatggtctcgtaacttgcgaaaaaattacattcaataaaactaccgg
acaattggatcaaaacactgcaggaaaatataagataccaaaagccagtgatgttccaaa
agactttagagtgaaacttctgggcattaataatgcaaatggagcacaagtttactcgtc
aaaaggaatcggagagccaccactaatgatgtcatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCATGGATGTCGGGGAAAGCTTG 479 469 22 Antisense
TCGGGGAAAGCTTGAATCCGGCCCT 286 603 33 Antisense
TGAATCCGGCCCTCGATATTGGACA 102 573 45 Antisense
GGATATGGTCTCGTAACTTGCGAAA 153 511 92 Antisense
TAAAACTACCGGACAATTGGATCAA 131 639 130 Antisense
AATTGGATCAAAACACTGCAGGAAA 352 179 144 Antisense
GATACCAAAAGCCAGTGATGTTCCA 196 425 175 Antisense
GAGTGAAACTTCTGGGCATTAATAA 373 401 210 Antisense
AATGGAGCACAAGTTTACTCGTCAA 613 161 239 Antisense
AAGGAATCGGAGAGCCACCACTAAT 10 159 264 Antisense
GAGAGCCACCACTAATGATGTCATG 154 395 273 Antisense
Sequence Source Affymetrix Proprietary Database