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[174727_at]

Description CEK053F1R_rc /REP_DB=TREMBL Accession /GB=D64511 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk53f1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 48      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 82      6grp_total > 49      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 364
(3)
P 		186.3
(-3)
P 		281.5
(0.2)
P 		283.5
(0.3)
P 		178
B 339.5
(3)
P 		240.6
(-1.9)
P 		217.2
(-3)
P 		254.3
(-1.2)
P 		122
C 362.9
(3)
P 		264.1
(-3)
P 		303.4
(-0.6)
P 		361.9
(2.9)
P 		99
avr 355.5
(3) 		230.3
(-3) 		267.4
(-1.2) 		299.9
(0.3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 403.6
(2.4)
P 		272.4
(-1.7)
P 		353.4
(0.8)
P 		228.3
(-3)
P 		424.8
(3)
P 		346.6
(0.6)
P 		197
B 445.4
(2.4)
P 		393.2
(0.7)
P 		310.9
(-1.9)
P 		277.1
(-3)
P 		354.1
(-0.5)
P 		465.1
(3)
P 		188
C 416.4
(2)
P 		400.5
(1.5)
P 		276.6
(-3)
P 		315.3
(-1.6)
P 		290.5
(-2.5)
P 		443.4
(3)
P 		167
avr 421.8
(3) 		355.4
(0.3) 		313.6
(-1.4) 		273.6
(-3) 		356.5
(0.4) 		418.4
(2.9) 		148
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 132.5
(3)
P 		40.4
(-3)
P 		73.6
(-0.8)
P 		41.5
(-2.9)
P 		92
B 108.3
(3)
P 		59.4
(-2.1)
P 		50.3
(-3)
P 		54.2
(-2.6)
P 		58
C 120.1
(3)
P 		75.9
(-1.5)
P 		105.8
(1.5)
P 		61
(-3)
P 		59
avr 120.3
(3) 		58.6
(-2.4) 		76.6
(-0.9) 		52.2
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 74.4
(-1.6)
P 		96.9
(0.3)
P 		77
(-1.4)
P 		57.9
(-3)
P 		128.2
(3)
P 		84.4
(-0.7)
P 		70
B 155
(1.9)
P 		130.9
(0.5)
P 		75.5
(-2.9)
P 		73.4
(-3)
P 		143.9
(1.2)
P 		173.4
(3)
P 		100
C 154.3
(3)
P 		122.2
(0.9)
P 		70.4
(-2.5)
P 		63.4
(-3)
P 		94
(-1)
P 		148.4
(2.6)
P 		91
avr 127.9
(2.4) 		116.7
(1.4) 		74.3
(-2.2) 		64.9
(-3) 		122
(1.9) 		135.4
(3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20724
Sequence Type Exemplar sequence
Representative Public ID CEK053F1R NCBI
Target Description CEK053F1R_rc /REP_DB=TREMBL Accession /GB=D64511 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk53f1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062877(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062877 NCBI Caenorhabditis elegans C32D5.10 (C32D5.10) mRNA, complete cds. 11/11 None
C32D5.10 ENSEMBL cdna:known chromosome:CEL140:II:6349170:6351934:-1 gene:C32D5.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000026846 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6349176-6349508(-) 92.77 100.0
Public Domain and Genome References
Gene Title
Ensembl C32D5.10
Entrez Gene 174051 Entrez gene
SwissProt Q09268 EMBL-EBI
WormBase CE29688 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46798 Hypothetical protein C32D5.10 [Caenorhabditis elegans] ref|NP_495278.2| C32D5.10 [Caenorhabditis elegans] sp|Q09268|YQDA_CAEEL Hypothetical RING finger protein C32D5.10 in chromosome II 0.0
blast T15741 hypothetical protein C32D5.10 - Caenorhabditis elegans 0.0
blast CAE59299 Hypothetical protein CBG02634 [Caenorhabditis briggsae] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 1.0E-9
Sequence
Target Sequence 
>C. ELEGANS:174727_AT
aaggcggatatcgacgagttttcaaactttttcctaggcatatcataattttttacaact
tttttacttttattttttctgacatttcgatgtttcgtagtagcaagtttccaaatgttc
catcctgggctacaagcgaacttattcggcaatttttagattttcgttgtttacggttca
aagaaaaaagcgtttttcatatttttattgtttctccatgttagctatcattttttattt
atttatatttatagctttattctgattattgtact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCGGATATCGACGAGTTTTCAA 607 161 40 Antisense
TTTCAAACTTTTTCCTAGGCATATC 404 671 59 Antisense
TTTCTGACATTTCGATGTTTCGTAG 131 671 115 Antisense
GATGTTTCGTAGTAGCAAGTTTCCA 491 409 128 Antisense
TCCAAATGTTCCATCCTGGGCTACA 72 589 149 Antisense
TGTTCCATCCTGGGCTACAAGCGAA 293 563 155 Antisense
GGGCTACAAGCGAACTTATTCGGCA 521 491 166 Antisense
TATTCGGCAATTTTTAGATTTTCGT 161 655 182 Antisense
ATATTTTTATTGTTTCTCCATGTTA 581 19 238 Antisense
GTTTCTCCATGTTAGCTATCATTTT 339 451 249 Antisense
ATAGCTTTATTCTGATTATTGTACT 638 21 290 Antisense
Sequence Source Affymetrix Proprietary Database