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[174726_at]

Description CEK031D1R_rc /REP_DB=TREMBL Accession /GB=D33461 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk31d1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 48      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 84      6grp_total > 49      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 773
1 2 3 4 range
A 603.4
(2.4)
P 		335.9
(-3)
P 		349.6
(-2.7)
P 		635.5
(3)
P 		300
B 739.4
(0.6)
P 		482.2
(-1.4)
P 		273
(-3)
P 		1045.5
(3)
P 		773
C 925.8
(3)
P 		472.7
(-2.2)
P 		402.4
(-3)
P 		907.9
(2.8)
P 		523
avr 756.2
(1.8) 		430.3
(-2) 		341.7
(-3) 		863
(3) 		521

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 626
1 2 3 4 5 6 range
A 785.3
(3)
P 		468.6
(-1)
P 		352.7
(-2.5)
P 		311.5
(-3)
P 		702.7
(2)
P 		720.1
(2.2)
P 		474
B 796.4
(3)
P 		496
(-0.9)
P 		551.6
(-0.2)
P 		340
(-3)
P 		525.7
(-0.6)
P 		793.4
(3)
P 		456
C 688.2
(0.6)
P 		513.6
(-1.1)
P 		312.5
(-3)
P 		391.5
(-2.2)
P 		564.3
(-0.6)
P 		937.4
(3)
P 		625
avr 756.6
(2.2) 		492.7
(-1.1) 		405.6
(-2.3) 		347.7
(-3) 		597.6
(0.2) 		817
(3) 		469
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 219.7
(3)
P 		72.9
(-3)
P 		91.4
(-2.2)
P 		93
(-2.2)
P 		147
B 235.8
(3)
P 		119.1
(-1.1)
P 		63.3
(-3)
P 		222.8
(2.5)
P 		173
C 306.5
(3)
P 		135.9
(-3)
P 		140.3
(-2.8)
P 		153.1
(-2.4)
P 		171
avr 254
(3) 		109.3
(-2.6) 		98.3
(-3) 		156.3
(-0.8) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 144.8
(0)
P 		166.7
(1)
P 		76.8
(-3)
P 		79
(-2.9)
P 		212
(3)
P 		175.3
(1.4)
P 		135
B 277.1
(2.5)
P 		165.1
(-0.8)
P 		133.9
(-1.7)
P 		90
(-3)
P 		213.6
(0.6)
P 		295.8
(3)
P 		206
C 255.1
(1.5)
P 		156.7
(-1)
P 		79.5
(-3)
P 		78.7
(-3)
P 		182.6
(-0.3)
P 		313.8
(3)
P 		235
avr 225.7
(1.8) 		162.8
(-0.3) 		96.7
(-2.5) 		82.6
(-3) 		202.7
(1) 		261.6
(3) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174726_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20332
Sequence Type Exemplar sequence
Representative Public ID CEK031D1R NCBI
Target Description CEK031D1R_rc /REP_DB=TREMBL Accession /GB=D33461 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk31d1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059919(11), NM_001026432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059919 NCBI Caenorhabditis elegans RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) (rskn-1) mRNA, complete cds. 11/11 None
NM_001026432 NCBI Caenorhabditis elegans RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) (rskn-1) mRNA, complete cds. 11/11 None
T01H8.1b ENSEMBL cdna:known chromosome:CEL140:I:8581993:8586155:-1 gene:T01H8.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010905 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172647 4 2 NM_001026432 174726_at (11)
NM_059919 174726_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8581933-8582294(-) 100.0 100.0
Public Domain and Genome References
Ensembl T01H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02301 Hypothetical protein T01H8.1b [Caenorhabditis elegans] ref|NP_492320.2| RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) [Caenorhabditis elegans] 0.0
blast CAA99896 Hypothetical protein T01H8.1a [Caenorhabditis elegans] emb|CAB02302.2| Hypothetical protein T01H8.1a [Caenorhabditis elegans] ref|NP_492319.2| RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) [Caenorhabditis elegans] sp|Q21734|KS6A_CAEEL Putative ribosomal protein S6 kinase alpha 0.0
blast CAE17895 Hypothetical protein T01H8.1c [Caenorhabditis elegans] emb|CAE17938.1| Hypothetical protein T01H8.1c [Caenorhabditis elegans] ref|NP_001021602.1| RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) [Caenorhabditis elegans] 0.0
blast CAI70402 Hypothetical protein T01H8.1d [Caenorhabditis elegans] emb|CAI70409.1| Hypothetical protein T01H8.1d [Caenorhabditis elegans] ref|NP_001021603.1| RSK-pNinety (RSK-p90 kinase) homolog family member (rskn-1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-48
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-68
hanks 1.6.3 AGC group; AGC VI phosphorylate ribosomal protein S6; RSK2N 1.0E-125
hanks 1.6.2 AGC group; AGC VI phosphorylate ribosomal protein S6; RSK1N 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-94
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-88
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 2.3E-18
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-94
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-72
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 2.3E-18
Sequence
Target Sequence 
>C. ELEGANS:174726_AT
aatactactaacatttatccaattgtttcaacaaatgctcctcattgaagcccaatcacc
aatttaaataactatttattgactccataattacattcatttcatccaaacaataactaa
ctttatttactcatcacactcatcccttccaaatcctctatcgcctatgttgtgattaaa
attgcgcatccaaaagaattttcagccaaatacgggattttctgcattgaatcatttatt
tccttctaattcttcatttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACTACTAACATTTATCCAATTG 648 123 26 Antisense
AACAAATGCTCCTCATTGAAGCCCA 638 133 55 Antisense
GAAGCCCAATCACCAATTTAAATAA 34 343 72 Antisense
TTGACTCCATAATTACATTCATTTC 606 705 104 Antisense
CATTCATTTCATCCAAACAATAACT 220 217 119 Antisense
ACTTTATTTACTCATCACACTCATC 325 101 145 Antisense
CAAATCCTCTATCGCCTATGTTGTG 594 193 175 Antisense
GTTGTGATTAAAATTGCGCATCCAA 576 437 194 Antisense
GCCAAATACGGGATTTTCTGCATTG 204 277 230 Antisense
GCATTGAATCATTTATTTCCTTCTA 410 307 249 Antisense
ATTTCCTTCTAATTCTTCATTTATT 589 17 263 Antisense
Sequence Source Affymetrix Proprietary Database