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[174721_at]

Description CEK014B2R_rc /REP_DB=TREMBL Accession /GB=D27567 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk14b2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 80      6grp_total > 09      4grp_total > 47      6grp_cgc > 0      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 235
1 2 3 4 range
A 320.6
(3)
P 		85.7
(-3)
P 		132.2
(-1.8)
P 		153.3
(-1.3)
P 		235
B 273.7
(3)
P 		126.5
(-3)
P 		126.1
(-3)
P 		162.2
(-1.5)
P 		148
C 271.7
(3)
P 		124.1
(-3)
P 		182.9
(-0.6)
P 		126.7
(-2.9)
P 		148
avr 288.7
(3) 		112.1
(-3) 		147.1
(-1.8) 		147.4
(-1.8) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 214
(0.7)
P 		243
(3)
P 		177
(-2.3)
P 		194.8
(-0.9)
P 		225.2
(1.6)
P 		168.4
(-3)
P 		75
B 291.8
(2.7)
P 		217.2
(-0.2)
P 		151.3
(-2.8)
P 		299.1
(3)
P 		145.5
(-3)
P 		174.7
(-1.9)
P 		154
C 356.1
(3)
P 		228.4
(-1.7)
P 		212.7
(-2.3)
P 		233.8
(-1.5)
P 		211.5
(-2.3)
P 		193
(-3)
P 		163
avr 287.3
(3) 		229.5
(-0.2) 		180.3
(-2.9) 		242.6
(0.5) 		194.1
(-2.1) 		178.7
(-3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 116.7
(3)
P 		18.6
(-3)
P 		34.6
(-2)
P 		22.4
(-2.8)
P 		98
B 87.3
(3)
P 		31.2
(-2.8)
P 		29.2
(-3)
P 		34.6
(-2.4)
P 		58
C 89.9
(3)
P 		35.7
(-1.7)
P 		63.7
(0.7)
P 		21.4
(-3)
P 		69
avr 98
(3) 		28.5
(-2.8) 		42.5
(-1.6) 		26.1
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 39.4
(-2.9)
P 		86.4
(3)
P 		38.5
(-3)
P 		49.4
(-1.6)
P 		68
(0.7)
P 		41
(-2.7)
P 		48
B 101.5
(3)
P 		72.3
(0.3)
P 		36.7
(-3)
P 		79.2
(0.9)
P 		59.1
(-0.9)
P 		65.1
(-0.4)
P 		65
C 132
(3)
P 		69.7
(-1.4)
P 		54.1
(-2.5)
P 		47
(-3)
P 		68.5
(-1.5)
P 		64.6
(-1.8)
P 		85
avr 91
(3) 		76.1
(1.1) 		43.1
(-3) 		58.5
(-1.1) 		65.2
(-0.2) 		56.9
(-1.3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174721_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20206
Sequence Type Exemplar sequence
Representative Public ID CEK014B2R NCBI
Target Description CEK014B2R_rc /REP_DB=TREMBL Accession /GB=D27567 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk14b2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059821(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059821 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-8) (rme-8) mRNA, complete cds. 11/11 A 
F18C12.2a ENSEMBL cdna:known chromosome:CEL140:I:8091709:8103720:1 gene:F18C12.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172594 2 1 NM_059821 174721_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8103306-8103641(+) 91.04 100.0
Public Domain and Genome References
Ensembl F18C12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99831 Hypothetical protein F18C12.2a [Caenorhabditis elegans] ref|NP_492222.2| Receptor Mediated Endocytosis family member (rme-8) [Caenorhabditis elegans] gb|AAK54248.1| endocytosis protein RME-8 [Caenorhabditis elegans] 0.0
blast CAA99832 Hypothetical protein F18C12.2b [Caenorhabditis elegans] ref|NP_001021395.1| Receptor Mediated Endocytosis family member (rme-8) [Caenorhabditis elegans] 0.0
blast CAE66544 Hypothetical protein CBG11852 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.99999986863986E-8
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.6E-14
Sequence
Target Sequence 
>C. ELEGANS:174721_AT
tgtgataatttttcgaagtgttcccccttgtcgcgtatctacttttgattatttttgatt
tcccgtgatttttttgtgaaaatgttgttcttctggtgcttttcccccattttcagcgga
ttttttcgacttttgttcttttttctttcgattgtttgtgcgcacgaaattttgggatcc
ctacacaagtctacggtgaacttttgacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGATAATTTTTCGAAGTGTTCCC 651 549 82 Antisense
CCTTGTCGCGTATCTACTTTTGATT 564 269 107 Antisense
GATTATTTTTGATTTCCCGTGATTT 668 429 128 Antisense
TTGATTTCCCGTGATTTTTTTGTGA 62 703 136 Antisense
AAATGTTGTTCTTCTGGTGCTTTTC 69 121 161 Antisense
CCATTTTCAGCGGATTTTTTCGACT 688 269 188 Antisense
TCAGCGGATTTTTTCGACTTTTGTT 371 621 194 Antisense
TTTCTTTCGATTGTTTGTGCGCACG 427 671 223 Antisense
GTGCGCACGAAATTTTGGGATCCCT 231 477 239 Antisense
GGATCCCTACACAAGTCTACGGTGA 166 511 256 Antisense
CAAGTCTACGGTGAACTTTTGACAA 610 157 267 Antisense
Sequence Source Affymetrix Proprietary Database