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[174720_at]

Description CEK026D7R_rc /REP_DB=TREMBL Accession /GB=D33117 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk26d7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 68      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 93      6grp_total > 69      4grp_total > 14      6grp_highest_sub > Cu      6grp_cgc > 28      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 563
1 2 3 4 range
A 472.8
(1.7)
P 		232.3
(-2.4)
P 		199
(-3)
P 		546.3
(3)
P 		347
B 492.1
(-0.1)
P 		234.7
(-3)
P 		238.5
(-3)
P 		761.8
(3)
P 		527
C 495.4
(1.2)
P 		234.2
(-3)
P 		307.7
(-1.8)
P 		604.7
(3)
P 		371
avr 486.8
(0.8) 		233.7
(-3) 		248.4
(-2.8) 		637.6
(3) 		404

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 353.3
(-1)
P 		444.5
(1.2)
P 		273.4
(-3)
P 		272.1
(-3)
P 		393
(-0)
P 		515.6
(3)
P 		244
B 458.4
(-0.3)
P 		466.8
(-0.1)
P 		408.9
(-1.3)
P 		324.9
(-3)
P 		388.5
(-1.7)
P 		621.2
(3)
P 		296
C 430.1
(-0.8)
P 		389.7
(-1.4)
P 		310.6
(-2.6)
P 		286.3
(-3)
P 		303.7
(-2.7)
P 		671.1
(3)
P 		385
avr 413.9
(-0.7) 		433.7
(-0.3) 		331
(-2.3) 		294.4
(-3) 		361.7
(-1.7) 		602.6
(3) 		308
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 172.2
(3)
P 		50.4
(-3)
P 		52
(-2.9)
P 		80
(-1.5)
P 		122
B 156.9
(2.7)
P 		58
(-2.8)
P 		55.3
(-3)
P 		162.3
(3)
P 		107
C 164
(3)
P 		67.3
(-3)
P 		107.2
(-0.5)
P 		102
(-0.8)
P 		97
avr 164.4
(3) 		58.6
(-3) 		71.5
(-2.3) 		114.8
(0.2) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 65.1
(-2.7)
P 		158.1
(3)
P 		59.5
(-3)
P 		69
(-2.4)
P 		118.6
(0.6)
P 		125.5
(1)
P 		99
B 159.5
(0)
P 		155.4
(-0.1)
P 		99.3
(-2.5)
P 		86
(-3)
P 		157.8
(-0)
P 		231.7
(3)
P 		146
C 159.4
(0.7)
P 		118.9
(-0.8)
P 		79.1
(-2.2)
P 		57.6
(-3)
P 		98.3
(-1.5)
P 		224.7
(3)
P 		167
avr 128
(-0.2) 		144.1
(0.6) 		79.3
(-2.6) 		70.9
(-3) 		124.9
(-0.4) 		194
(3) 		123
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174720_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22148
Sequence Type Exemplar sequence
Representative Public ID CEK026D7R NCBI
Target Description CEK026D7R_rc /REP_DB=TREMBL Accession /GB=D33117 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk26d7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076800(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076800 NCBI Caenorhabditis elegans Insulin receptor SubsTrate homolog family member (ist-1) (ist-1) mRNA, complete cds. 11/11 A 
C54D1.3 ENSEMBL cdna:known chromosome:CEL140:X:7132155:7139598:1 gene:C54D1.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7139252-7139587(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ist-1
Ensembl C54D1.3
Entrez Gene 180978 Entrez gene
SwissProt Q18820 EMBL-EBI
WormBase CE37098 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48153 Insulin receptor substrate homolog protein 1 [Caenorhabditis elegans] ref|NP_509201.3| Insulin receptor SubsTrate homolog family member (ist-1) [Caenorhabditis elegans] 0.0
blast AAL40865 insulin receptor substrate-like protein [Caenorhabditis elegans] 0.0
blast CAE65846 Hypothetical protein CBG10979 [Caenorhabditis briggsae] 1.0E-167
Sequence
Target Sequence 
>C. ELEGANS:174720_AT
ctagaagtttttaagcacctacacaaaacctcaaaaaattgacccatttcatctcttttt
ctactttccaatctcaacctttccttcattacctctttctctactcatttcatttttagt
tacaatttttcctagtttgcattcccatcaaccacatgtcggaagttttactgtgtttta
atatttgtgtcccgagtacttctatttttgagatctaacaacaaattgccgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTAGAAGTTTTTAAGCACCTACACA 306 225 57 Antisense
GCACCTACACAAAACCTCAAAAAAT 436 313 71 Antisense
AATTGACCCATTTCATCTCTTTTTC 189 179 93 Antisense
CAATTTTTCCTAGTTTGCATTCCCA 315 181 179 Antisense
CATTCCCATCAACCACATGTCGGAA 403 217 196 Antisense
ACATGTCGGAAGTTTTACTGTGTTT 295 103 210 Antisense
CTGTGTTTTAATATTTGTGTCCCGA 403 239 227 Antisense
ATTTGTGTCCCGAGTACTTCTATTT 553 13 239 Antisense
TGTCCCGAGTACTTCTATTTTTGAG 300 557 244 Antisense
GAGTACTTCTATTTTTGAGATCTAA 496 399 250 Antisense
TGAGATCTAACAACAAATTGCCGTT 508 551 265 Antisense
Sequence Source Affymetrix Proprietary Database