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[174719_at]

Description CEK025E6R_rc /REP_DB=TREMBL Accession /GB=D33056 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk25e6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 56      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 76      6grp_total > 47      4grp_total > 15      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 527
1 2 3 4 range
A 1167.4
(3)
P 		933.1
(-1.5)
P 		852.1
(-3)
P 		1073.8
(1.2)
P 		315
B 1022.3
(-1.3)
P 		892.2
(-3)
P 		894.6
(-3)
P 		1358.5
(3)
P 		466
C 1287.5
(3)
P 		831.4
(-3)
P 		1064.6
(0.1)
P 		1078.9
(0.3)
P 		456
avr 1159.1
(2.8) 		885.6
(-3) 		937.1
(-1.9) 		1170.4
(3) 		285

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 1097
(3)
P 		1065.9
(2)
P 		988.4
(-0.7)
P 		918.7
(-3)
P 		1026.2
(0.6)
P 		1032.2
(0.8)
P 		178
B 1108.1
(0.2)
P 		1110.9
(0.2)
P 		908.3
(-3)
P 		1060.6
(-0.6)
P 		909.6
(-3)
P 		1286.3
(3)
P 		378
C 1033.4
(-0.6)
P 		1092.2
(0.4)
P 		964.9
(-1.8)
P 		1060.7
(-0.2)
P 		894.2
(-3)
P 		1247.5
(3)
P 		353
avr 1079.5
(0.3) 		1089.7
(0.6) 		953.9
(-2.7) 		1013.3
(-1.3) 		943.3
(-3) 		1188.7
(3) 		245
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 425.1
(3)
P 		202.5
(-2)
P 		222.8
(-1.5)
P 		157.2
(-3)
P 		268
B 326
(3)
P 		220.4
(-2.3)
P 		207.3
(-3)
P 		289.5
(1.2)
P 		119
C 426.2
(3)
P 		239
(-1.6)
P 		371.1
(1.6)
P 		181.9
(-3)
P 		244
avr 392.4
(3) 		220.6
(-2.6) 		267.1
(-1.1) 		209.5
(-3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 202.2
(-3)
P 		379.1
(3)
P 		215.3
(-2.6)
P 		233
(-2)
P 		309.7
(0.6)
P 		251.3
(-1.3)
P 		177
B 385.6
(0.8)
P 		369.8
(0.5)
P 		220.6
(-3)
P 		280.9
(-1.6)
P 		369.6
(0.5)
P 		479.7
(3)
P 		259
C 383
(2)
P 		333.2
(0.5)
P 		245.6
(-2.1)
P 		213.3
(-3)
P 		289.4
(-0.8)
P 		417.6
(3)
P 		204
avr 323.6
(0.7) 		360.7
(2.1) 		227.2
(-3) 		242.4
(-2.4) 		322.9
(0.7) 		382.9
(3) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174719_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20351
Sequence Type Exemplar sequence
Representative Public ID CEK025E6R NCBI
Target Description CEK025E6R_rc /REP_DB=TREMBL Accession /GB=D33056 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk25e6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060186(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060186 NCBI Caenorhabditis elegans GEI-4(Four) Interacting protein family member (gfi-2) (gfi-2) mRNA, complete cds. 11/11 None
K02A11.1b.1 ENSEMBL cdna:known chromosome:CEL140:I:9745939:9752263:1 gene:K02A11.1 11/11 A 
K02A11.1b.2 ENSEMBL cdna:known chromosome:CEL140:I:9748666:9752263:1 gene:K02A11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172825 2 1 NM_060186 174719_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9751828-9752158(+) 98.8 98.8
Public Domain and Genome References
Ensembl K02A11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC42316 Hypothetical protein K02A11.1b [Caenorhabditis elegans] ref|NP_492587.1| GEI-4(Four) Interacting protein family member (gfi-2) [Caenorhabditis elegans] 0.0
blast CAA99881 Hypothetical protein K02A11.1a [Caenorhabditis elegans] ref|NP_492588.1| GEI-4(Four) Interacting protein family member (gfi-2) [Caenorhabditis elegans] 0.0
blast CAE60239 Hypothetical protein CBG03811 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 3.3
Pfam IPR002110 EMBL-EBI Ankyrin 2.4E-9
Pfam IPR002110 EMBL-EBI Ankyrin 4.2E-10
Pfam IPR002110 EMBL-EBI Ankyrin 1.0E-6
Pfam IPR002110 EMBL-EBI Ankyrin 6.7E-5
Sequence
Target Sequence 
>C. ELEGANS:174719_AT
ccctcaaatcatttctctactgtgatcgccgccttctctctccgaactttggtcattttc
tcttaaaacgtttagtcccaaatctatatatttatatatgtatttcttttatcgaaatgt
gtaagacctcttgatatatccatattttctgctgataattctaaaacggttgtgaactgt
ctaattgcttcttgaaaacccccaaaaatccttcacctttctctctaaatttctcctcac
atttatgacagaatttgtttgttttcgaattttcttcttccatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCTCAAATCATTTCTCTACTGTGA 558 259 13 Antisense
TCTCTCTCCGAACTTTGGTCATTTT 212 613 47 Antisense
TCCGAACTTTGGTCATTTTCTCTTA 558 595 53 Antisense
AACGTTTAGTCCCAAATCTATATAT 457 143 79 Antisense
TATCGAAATGTGTAAGACCTCTTGA 540 651 122 Antisense
AAGACCTCTTGATATATCCATATTT 53 155 135 Antisense
ATCCATATTTTCTGCTGATAATTCT 9 41 150 Antisense
ACGGTTGTGAACTGTCTAATTGCTT 187 91 178 Antisense
TCTAATTGCTTCTTGAAAACCCCCA 104 613 192 Antisense
AATTTCTCCTCACATTTATGACAGA 364 175 240 Antisense
GTTTTCGAATTTTCTTCTTCCATCA 256 449 273 Antisense
Sequence Source Affymetrix Proprietary Database