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[174686_at]

Description CEK018G5R_rc /REP_DB=TREMBL Accession /GB=D32563 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk18g5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      6grp_highest_nonorm > 50      6grp_total > 03      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 41      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 722
1 2 3 4 range
A 724.4
(3)
P 		394.4
(0)
P 		320.6
(-0.6)
P 		58.4
(-3)
A 		666
B 780.1
(3)
P 		538.3
(0.8)
P 		383.5
(-0.7)
P 		131.7
(-3)
A 		648
C 699
(3)
P 		474.6
(0.7)
P 		421.4
(0.2)
P 		111.7
(-3)
A 		587
avr 734.5
(3) 		469.1
(0.5) 		375.2
(-0.4) 		100.6
(-3) 		634

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 698
1 2 3 4 5 6 range
A 861.4
(3)
P 		786
(2.2)
P 		385.6
(-1.9)
P 		395.2
(-1.8)
P 		600.5
(0.3)
P 		283.7
(-3)
P 		578
B 641.6
(2.9)
P 		651.6
(3)
P 		213.1
(-3)
P 		596.3
(2.2)
P 		489.3
(0.8)
P 		267.6
(-2.3)
P 		439
C 848.1
(2.5)
P 		911.2
(3)
P 		599.9
(0.3)
P 		420
(-1.3)
P 		541.7
(-0.2)
P 		219
(-3)
P 		692
avr 783.7
(3) 		782.9
(3) 		399.5
(-1.4) 		470.5
(-0.6) 		543.8
(0.3) 		256.8
(-3) 		527
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 263.7
(3)
P 		85.6
(-1.2)
P 		83.8
(-1.2)
P 		8.5
(-3)
A 		255
B 248.8
(3)
P 		133
(-0.1)
P 		88.9
(-1.3)
P 		28.1
(-3)
A 		221
C 231.4
(3)
P 		136.5
(0.3)
P 		146.9
(0.6)
P 		18.8
(-3)
A 		213
avr 248
(3) 		118.4
(-0.4) 		106.5
(-0.7) 		18.5
(-3) 		230

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 158.8
(-0.4)
P 		279.5
(3)
P 		84
(-2.6)
P 		100.2
(-2.1)
P 		181.2
(0.2)
P 		69.1
(-3)
P 		210
B 223.3
(3)
P 		216.9
(2.8)
P 		51.7
(-3)
P 		157.9
(0.7)
P 		198.8
(2.1)
P 		99.8
(-1.3)
P 		172
C 314.3
(3)
P 		278
(2.1)
P 		152.7
(-1)
P 		84.5
(-2.7)
P 		175.3
(-0.5)
P 		73.3
(-3)
P 		241
avr 232.1
(2.1) 		258.1
(3) 		96.1
(-2.5) 		114.2
(-1.9) 		185.1
(0.5) 		80.7
(-3) 		177
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174686_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20181
Sequence Type Exemplar sequence
Representative Public ID CEK018G5R NCBI
Target Description CEK018G5R_rc /REP_DB=TREMBL Accession /GB=D32563 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk18g5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025814 NCBI Caenorhabditis elegans UNCoordinated family member (unc-89) (unc-89) mRNA, complete cds. 11/11 None
C09D1.1b ENSEMBL cdna:known chromosome:CEL140:I:4035894:4090875:1 gene:C09D1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171990 7 1 NM_001025813 175353_s_at (11), 192998_at (11)
NM_001025814 174686_at (11), 175353_s_at (11), 189196_at (11), 192998_at (11)
NM_001025815 189196_at (11)
NM_001025816 189196_at (11)
NM_001025817 174492_at (11), 192998_at (11)
NM_001025818 189196_at (11), 192998_at (11)
NM_001025819 175353_s_at (11), 192998_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4090479-4090865(+) 94.04 100.0
Public Domain and Genome References
Ensembl C09D1.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP68958 Uncoordinated protein 89, isoform b [Caenorhabditis elegans] ref|NP_001020985.1| UNCoordinated family member (unc-89) [Caenorhabditis elegans] 0.0
blast AAV34800 Uncoordinated protein 89, isoform f [Caenorhabditis elegans] ref|NP_001020989.1| UNCoordinated family member (unc-89) [Caenorhabditis elegans] 0.0
blast AAV34801 Uncoordinated protein 89, isoform g [Caenorhabditis elegans] ref|NP_001020990.1| UNCoordinated family member (unc-89) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 4.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.4E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.3E-12
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-41
Pfam IPR000719 EMBL-EBI Protein kinase 2.9E-30
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.9E-8
Pfam IPR007850 EMBL-EBI RCSD 1.4E-35
Pfam IPR007850 EMBL-EBI RCSD 2.0E-50
Pfam IPR007850 EMBL-EBI RCSD 7.5E-38
Pfam IPR007850 EMBL-EBI RCSD 1.1E-30
Pfam IPR007850 EMBL-EBI RCSD 3.4E-11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.8E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 8.6E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.021
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.34
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.024
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0023
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.0E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.68
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.8E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.15
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.9E-11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.4E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.12
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0069
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.1E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.4E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.6E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.9E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.1E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.2E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.2E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 8.6E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.77
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.06
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.9E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.1E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.018
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.069
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0093
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.3
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.066
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.4E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.014
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.6E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.7E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.4E-11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.1E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.4E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 7.7E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 7.9E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.6E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.018
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0098
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 7.5E-6
Sequence
Target Sequence 
>C. ELEGANS:174686_AT
ctttttggatacaatctgtgcccgagattttccaaattaatagtcgaatttatcgtttct
acgagtagatttttattcatttttgaacttttttttaactttaaaagccttcaacgactt
tttgcctattttgtgctttctgtttcccgaatctgcttaattttctcttgtgcccaagga
gaaattgagtggggtgggaaacttgtgttaaatgttttttttttaatttttatttatgca
acgatgattaaattgtaattagaatagcttcatatttctattttcataactttttaatga
taaccttcttccttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTTTTGGATACAATCTGTGCCCGA 283 223 44 Antisense
TCTGTGCCCGAGATTTTCCAAATTA 695 605 58 Antisense
ATAGTCGAATTTATCGTTTCTACGA 298 3 83 Antisense
CTTCAACGACTTTTTGCCTATTTTG 84 221 152 Antisense
TGCCTATTTTGTGCTTTCTGTTTCC 577 585 166 Antisense
TTTCTGTTTCCCGAATCTGCTTAAT 618 671 180 Antisense
ATCTGCTTAATTTTCTCTTGTGCCC 94 35 194 Antisense
TAATTTTCTCTTGTGCCCAAGGAGA 388 633 201 Antisense
TGAGTGGGGTGGGAAACTTGTGTTA 651 565 229 Antisense
AGAATAGCTTCATATTTCTATTTTC 655 75 304 Antisense
TTTAATGATAACCTTCTTCCTTCCA 284 673 336 Antisense
Sequence Source Affymetrix Proprietary Database