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[174685_at]

Description CEK024B7R_rc /REP_DB=TREMBL Accession /GB=D32964 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk24b7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 30      6grp_total > 26      4grp_total > 15      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 1029.2
(3)
P 		510.7
(-3)
P 		553.8
(-2.5)
P 		951.3
(2.1)
P 		519
B 832.7
(3)
P 		524.9
(-3)
P 		607.9
(-1.4)
P 		805.4
(2.5)
P 		308
C 899.3
(3)
P 		624.1
(-3)
P 		777.6
(0.3)
P 		799.4
(0.8)
P 		275
avr 920.4
(3) 		553.2
(-3) 		646.4
(-1.5) 		852
(1.9) 		367

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1003
1 2 3 4 5 6 range
A 1009.9
(3)
P 		904.7
(1.6)
P 		740.3
(-0.5)
P 		586.4
(-2.5)
P 		652.3
(-1.6)
P 		547.8
(-3)
P 		462
B 1518.9
(3)
P 		1028.2
(-0.2)
P 		784.1
(-1.8)
P 		788.9
(-1.7)
P 		596.2
(-3)
P 		892
(-1.1)
P 		923
C 1070
(2.7)
P 		1103.6
(3)
P 		739.5
(-0.7)
P 		685.8
(-1.3)
P 		515.6
(-3)
P 		748.4
(-0.6)
P 		588
avr 1199.6
(3) 		1012.2
(1.2) 		754.6
(-1.4) 		687
(-2) 		588
(-3) 		729.4
(-1.6) 		612
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 264
1 2 3 4 range
A 374.7
(3)
P 		110.9
(-3)
P 		144.8
(-2.2)
P 		139.2
(-2.4)
P 		264
B 265.5
(3)
P 		129.6
(-3)
P 		140.9
(-2.5)
P 		171.6
(-1.1)
P 		136
C 297.7
(3)
P 		179.4
(-1.4)
P 		271
(2)
P 		134.8
(-3)
P 		163
avr 312.6
(3) 		140
(-3) 		185.6
(-1.4) 		148.5
(-2.7) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 395
1 2 3 4 5 6 range
A 186.2
(-1.3)
P 		321.8
(3)
P 		161.2
(-2.1)
P 		148.7
(-2.5)
P 		196.8
(-1)
P 		133.4
(-3)
P 		188
B 528.5
(3)
P 		342.2
(-0.3)
P 		190.4
(-3)
P 		209
(-2.7)
P 		242.2
(-2.1)
P 		332.6
(-0.5)
P 		338
C 396.6
(3)
P 		336.7
(1.6)
P 		188.2
(-1.8)
P 		137.9
(-3)
P 		166.8
(-2.3)
P 		250.5
(-0.4)
P 		259
avr 370.4
(3) 		333.6
(1.9) 		179.9
(-2.6) 		165.2
(-3) 		201.9
(-1.9) 		238.8
(-0.8) 		205
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174685_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21294
Sequence Type Exemplar sequence
Representative Public ID CEK024B7R NCBI
Target Description CEK024B7R_rc /REP_DB=TREMBL Accession /GB=D32964 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk24b7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069127(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069127 NCBI Caenorhabditis elegans T28C6.7 (T28C6.7) mRNA, complete cds. 11/11 None
T28C6.7 ENSEMBL cdna:known chromosome:CEL140:IV:8827786:8840638:1 gene:T28C6.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8840253-8840634(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28C6.7
Entrez Gene 177697 Entrez gene
SwissProt Q22847 EMBL-EBI
WormBase CE32942 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.12875.1.S1_AT similar to centrosomal protein 2 cfa
CHICKEN:GGA.7522.1.S1_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
CHICKEN:GGAAFFX.1161.2.S1_S_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
CHICKEN:GGAAFFX.1161.3.S1_S_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
HG-U133_PLUS_2:209495_AT centrosomal protein 2 hs
HG-U133A:209495_AT centrosomal protein 2 hs
HG-U133A_2:209495_AT centrosomal protein 2 hs
HG-U133_PLUS_2:210894_S_AT centrosomal protein 2 hs
HG-U133A:210894_S_AT centrosomal protein 2 hs
HG-FOCUS:210894_S_AT centrosomal protein 2 hs
HG-U133A_2:210894_S_AT centrosomal protein 2 hs
HG-U95AV2:35206_AT centrosomal protein 2 hs
HU35KSUBB:RC_R87989_AT centrosomal protein 2 hs
HU35KSUBC:RC_AA251153_AT centrosomal protein 2 hs
U133_X3P:G2832236_3P_AT centrosomal protein 2 hs
U133_X3P:G12655156_3P_S_AT centrosomal protein 2 hs
HG-U95C:56138_AT centrosomal protein 2 hs
HG-U95C:56139_AT centrosomal protein 2 hs
HG-U95E:74643_AT centrosomal protein 2 hs
MOUSE430_2:1421512_AT centrosomal protein 2 mm
MOUSE430A_2:1421512_AT centrosomal protein 2 mm
MOE430A:1421512_AT centrosomal protein 2 mm
MU11KSUBA:U33198_S_AT centrosomal protein 2 mm
MG-U74AV2:101145_AT centrosomal protein 2 mm
MG-U74CV2:135659_AT PREDICTED: centrosomal protein 2 [Mus musculus], mRNA sequence mm
MG-U74CV2:169516_AT PREDICTED: centrosomal protein 2 [Mus musculus], mRNA sequence mm
RAE230A:1377062_AT similar to centrosomal Nek2-associated protein 1 rn
RAT230_2:1377062_AT similar to centrosomal Nek2-associated protein 1 rn
Protein Similarities
Method ID Description E-Value
blast CAA90998 Hypothetical protein T28C6.7 [Caenorhabditis elegans] ref|NP_501528.2| T28C6.7 [Caenorhabditis elegans] 0.0
blast XP_653447 Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] gb|EAL48061.1| Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] 1.0E-43
blast CAE56384 Hypothetical protein CBG24066 [Caenorhabditis briggsae] emb|CAE57042.1| Hypothetical protein CBG24928 [Caenorhabditis briggsae] 2.0E-42
Sequence
Target Sequence 
>C. ELEGANS:174685_AT
cttttattctaggtctgtcagttctgtaggatccctaattaagtaacttttcctcaagta
ttttttaatgtatattcgtagatctgctaaacctaaattgaatcgataatttttggaaac
cttcttttcgagtcgataatatacatgagcgttctgttttatagttttcatccagatgac
ttgcccatacgtcatcgtcaagagatgactgattaatttattattttttacatccaaagt
gccttgatatcggtagctaatcgagttttttgaagggttttcatttgttttcttgtttat
aatgcacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTTTATTCTAGGTCTGTCAGTTCT 667 221 52 Antisense
GTATATTCGTAGATCTGCTAAACCT 579 451 121 Antisense
GAAACCTTCTTTTCGAGTCGATAAT 449 351 167 Antisense
GTCGATAATATACATGAGCGTTCTG 443 473 183 Antisense
GCGTTCTGTTTTATAGTTTTCATCC 175 291 200 Antisense
TTCATCCAGATGACTTGCCCATACG 168 689 218 Antisense
TTGCCCATACGTCATCGTCAAGAGA 92 701 232 Antisense
TATTTTTTACATCCAAAGTGCCTTG 151 659 273 Antisense
AAGTGCCTTGATATCGGTAGCTAAT 591 157 288 Antisense
TGAAGGGTTTTCATTTGTTTTCTTG 38 575 322 Antisense
TTGTTTTCTTGTTTATAATGCACGA 55 707 336 Antisense
Sequence Source Affymetrix Proprietary Database