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[174681_at] RAS-related protein

Description CEK063H3R_rc /REP_DB=TREMBL Accession /GB=D71225 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk63h3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 17      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 05      6grp_total > 17      4grp_total > 35      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2056
1 2 3 4 range
A 6860.5
(-1.7)
P 		6908.6
(-1.3)
P 		6686.4
(-3)
P 		7483.2
(3)
P 		797
B 5872.7
(-3)
P 		7253.7
(1)
P 		6768.2
(-0.4)
P 		7920.7
(3)
P 		2048
C 7857.8
(3)
P 		7534.1
(2)
P 		6180.5
(-2.1)
P 		5865.2
(-3)
P 		1993
avr 6863.7
(-0.2) 		7232.1
(3) 		6545
(-3) 		7089.7
(1.8) 		687

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5264
1 2 3 4 5 6 range
A 10220.8
(3)
P 		5431.1
(-2.8)
P 		6166.3
(-1.9)
P 		5229.7
(-3)
P 		6953.6
(-0.9)
P 		5457
(-2.7)
P 		4991
B 9312.1
(3)
P 		7107.5
(-0)
P 		6647.1
(-0.7)
P 		8019.5
(1.2)
P 		4956.9
(-3)
P 		6530.1
(-0.8)
P 		4355
C 7019.2
(0.8)
P 		8163.1
(3)
P 		5442.2
(-2.2)
P 		5042.2
(-3)
P 		5542.2
(-2)
P 		6443.9
(-0.3)
P 		3121
avr 8850.7
(3) 		6900.6
(-0.9) 		6085.2
(-2.5) 		6097.1
(-2.4) 		5817.6
(-3) 		6143.7
(-2.4) 		3033
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1612
1 2 3 4 range
A 2497.9
(3)
P 		1499.7
(-1.3)
P 		1748
(-0.2)
P 		1095.4
(-3)
P 		1403
B 1872.8
(3)
P 		1791.5
(1.4)
P 		1568.3
(-3)
P 		1687.9
(-0.6)
P 		305
C 2601.1
(3)
P 		2166.1
(1.4)
P 		2154.1
(1.3)
P 		989
(-3)
P 		1612
avr 2323.9
(3) 		1819.1
(0.2) 		1823.5
(0.2) 		1257.4
(-3) 		1067

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2226
1 2 3 4 5 6 range
A 1884.1
(1.3)
P 		1931.6
(1.7)
P 		1342.9
(-2.9)
P 		1326.4
(-3)
P 		2098.3
(3)
P 		1328.6
(-3)
P 		772
B 3240.1
(3)
P 		2365.8
(-0.2)
P 		1614.1
(-3)
P 		2124.1
(-1.1)
P 		2013.9
(-1.5)
P 		2435.1
(0)
P 		1626
C 2601.7
(3)
P 		2490.8
(2.6)
P 		1385.1
(-1.6)
P 		1014
(-3)
P 		1793.4
(-0.1)
P 		2157.1
(1.3)
P 		1588
avr 2575.3
(3) 		2262.7
(1.3) 		1447.4
(-3) 		1488.2
(-2.8) 		1968.5
(-0.2) 		1973.6
(-0.2) 		1128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174681_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20057
Sequence Type Exemplar sequence
Representative Public ID CEK063H3R NCBI
Target Description CEK063H3R_rc /REP_DB=TREMBL Accession /GB=D71225 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk63h3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058832(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058832 NCBI Caenorhabditis elegans UNCoordinated family member (unc-108) (unc-108) mRNA, complete cds. 11/11 None
F53F10.4.2 ENSEMBL cdna:known chromosome:CEL140:I:3824948:3826508:1 gene:F53F10.4 11/11 None
F53F10.4.1 ENSEMBL cdna:known chromosome:CEL140:I:3824949:3826516:1 gene:F53F10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3826163-3826498(+) 83.88 100.0
Public Domain and Genome References
Gene Title RAS-related protein
Gene Symbol unc-108
Ensembl F53F10.4
Entrez Gene 171956 Entrez gene
SwissProt O01577 EMBL-EBI
WormBase CE10986 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17949_S_AT Rab2-like GTP-binding protein (RAB2) at
ATGENOME1:17948_I_AT Rab2-like GTP-binding protein (RAB2) at
ATH1-121501:245407_AT Rab2-like GTP-binding protein (RAB2) at
CANINE:1582711_S_AT RAB2, member RAS oncogene family cfa
CANINE_2:CFA.3880.1.A1_S_AT RAB2, member RAS oncogene family cfa
CANINE_2:CFA.3880.1.A2_AT RAB2, member RAS oncogene family cfa
CANINE:1587782_AT RAB2, member RAS oncogene family cfa
CANINE:1594278_AT RAB2, member RAS oncogene family cfa
DROSOPHILA_2:1636607_AT Rab-protein 2 dm
DROSGENOME1:151803_AT Rab-protein 2 dm
CHICKEN:GGA.13000.1.S1_AT RAB2, member RAS oncogene family gga
CHICKEN:GGA.4794.1.S1_AT RAB2, member RAS oncogene family gga
HG-U133_PLUS_2:208733_AT RAB2, member RAS oncogene family hs
HG-U133A_2:208733_AT RAB2, member RAS oncogene family hs
HG-U133A:208733_AT RAB2, member RAS oncogene family hs
HG-U133_PLUS_2:208731_AT RAB2, member RAS oncogene family hs
HG-U133A_2:208731_AT RAB2, member RAS oncogene family hs
HG-U133A:208731_AT RAB2, member RAS oncogene family hs
HG-U133A:208734_X_AT RAB2, member RAS oncogene family hs
HG-U133_PLUS_2:208734_X_AT RAB2, member RAS oncogene family hs
HG-U133A_2:208734_X_AT RAB2, member RAS oncogene family hs
HG-U133_PLUS_2:208730_X_AT RAB2, member RAS oncogene family hs
HG-U133A_2:208730_X_AT RAB2, member RAS oncogene family hs
HG-U133A:208730_X_AT RAB2, member RAS oncogene family hs
HU35KSUBD:RC_R38819_AT RAB2, member RAS oncogene family hs
HG-U95AV2:623_S_AT RAB2, member RAS oncogene family hs
HC-G110:623_S_AT RAB2, member RAS oncogene family hs
HUGENEFL:X12953_AT RAB2, member RAS oncogene family hs
HUGENEFL:M28213_S_AT RAB2, member RAS oncogene family hs
U133_X3P:G550061_3P_A_AT RAB2, member RAS oncogene family hs
U133_X3P:HS.78305.0.A5_3P_AT RAB2, member RAS oncogene family hs
U133_X3P:HS.78305.0.A3_3P_AT RAB2, member RAS oncogene family hs
U133_X3P:HS.78305.0.A1_3P_AT RAB2, member RAS oncogene family hs
HG-U133_PLUS_2:208732_AT RAB2, member RAS oncogene family hs
HG-U133A_2:208732_AT RAB2, member RAS oncogene family hs
HG-FOCUS:208732_AT RAB2, member RAS oncogene family hs
HG-U133A:208732_AT RAB2, member RAS oncogene family hs
HU35KSUBD:RC_AA479949_AT RAB2, member RAS oncogene family hs
U133_X3P:HS.78305.0.A4_3P_AT RAB2, member RAS oncogene family hs
HG-U95AV2:32581_AT RAB2, member RAS oncogene family hs
HG-U133A_2:221960_S_AT RAB2, member RAS oncogene family hs
HG-U133_PLUS_2:221960_S_AT RAB2, member RAS oncogene family hs
HG-U133A:221960_S_AT RAB2, member RAS oncogene family hs
HG-U95D:67490_AT RAB2, member RAS oncogene family hs
HG-U95D:75891_AT RAB2, member RAS oncogene family hs
U133_X3P:HS.78305.1.A1_3P_A_AT RAB2, member RAS oncogene family hs
MOUSE430A_2:1436477_X_AT RAB2, member RAS oncogene family mm
MOE430A:1436477_X_AT RAB2, member RAS oncogene family mm
MOUSE430_2:1436477_X_AT RAB2, member RAS oncogene family mm
MU11KSUBA:C80220_RC_AT RAB2, member RAS oncogene family mm
MU11KSUBB:X95403_S_AT RAB2, member RAS oncogene family mm
MG-U74AV2:94031_AT RAB2, member RAS oncogene family mm
MG-U74AV2:162141_R_AT RAB2, member RAS oncogene family mm
MOE430A:1419945_S_AT RAB2, member RAS oncogene family mm
MOUSE430A_2:1419945_S_AT RAB2, member RAS oncogene family mm
MOUSE430_2:1419945_S_AT RAB2, member RAS oncogene family mm
MOE430A:1418621_AT RAB2, member RAS oncogene family mm
MOUSE430A_2:1418621_AT RAB2, member RAS oncogene family mm
MOUSE430_2:1418621_AT RAB2, member RAS oncogene family mm
MOE430A:1418622_AT RAB2, member RAS oncogene family mm
MOUSE430A_2:1418622_AT RAB2, member RAS oncogene family mm
MOUSE430_2:1418622_AT RAB2, member RAS oncogene family mm
MOE430A:1418623_AT RAB2, member RAS oncogene family mm
MOUSE430A_2:1418623_AT RAB2, member RAS oncogene family mm
MOUSE430_2:1418623_AT RAB2, member RAS oncogene family mm
MOE430A:1419946_S_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MOUSE430A_2:1419946_S_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MOUSE430_2:1419946_S_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MOE430A:1449676_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MOUSE430A_2:1449676_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MOUSE430_2:1449676_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
MU19KSUBB:TC30361_AT RAB2, member RAS oncogene family (Rab2), mRNA mm
RG-U34C:RC_AI237381_AT RAB2, member RAS oncogene family rn
RAE230A:1370087_AT RAB2, member RAS oncogene family rn
RAT230_2:1370087_AT RAB2, member RAS oncogene family rn
RG-U34A:RC_AI009095_AT RAB2, member RAS oncogene family rn
RAE230B:1392681_AT RAB2, member RAS oncogene family rn
RAT230_2:1392681_AT RAB2, member RAS oncogene family rn
RG-U34C:RC_AI112132_AT RAB2, member RAS oncogene family rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
160 two-component signal transduction system (phosphorelay) inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52431 Uncoordinated protein 108 [Caenorhabditis elegans] ref|NP_491233.1| UNCoordinated family member (unc-108) [Caenorhabditis elegans] 1.0E-102
blast CAE66672 Hypothetical protein CBG12011 [Caenorhabditis briggsae] 1.0E-101
blast CAA51234 RAB2 [Lymnaea stagnalis] sp|Q05975|RAB2_LYMST Ras-related protein Rab-2 1.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.2E-100
Sequence
Target Sequence 
>C. ELEGANS:174681_AT
tctctcttccagaaccataattttactttacatatatattgcaattgtctcttattacta
ccatcatttactgtatattgtcgcctgctttttctctttctcctctctttctctattttc
tctccttttcattcagtctctcaccaattgtgtgaatatctgaaattcctgtgattttcc
actcaatccatcagaacaacatgttgtgaaaatcccgctctctctccctcatttcctctt
aaaagtttgtatgtaagctctacttcttttttcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCTCTTCCAGAACCATAATTTTA 227 613 13 Antisense
ATTGCAATTGTCTCTTATTACTACC 602 3 50 Antisense
GTCTCTTATTACTACCATCATTTAC 540 469 59 Antisense
TCATTTACTGTATATTGTCGCCTGC 394 619 76 Antisense
CTGTATATTGTCGCCTGCTTTTTCT 214 239 83 Antisense
CATTCAGTCTCTCACCAATTGTGTG 110 217 142 Antisense
AAATTCCTGTGATTTTCCACTCAAT 633 115 175 Antisense
TCCACTCAATCCATCAGAACAACAT 541 587 190 Antisense
ACATGTTGTGAAAATCCCGCTCTCT 602 105 211 Antisense
TCCCTCATTTCCTCTTAAAAGTTTG 248 597 237 Antisense
ATGTAAGCTCTACTTCTTTTTTCCT 689 47 263 Antisense
Sequence Source Affymetrix Proprietary Database