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[174675_at] long-chain-fatty-acid-CoA ligase

Description CEK072F9R_rc /REP_DB=TREMBL Accession /GB=D66039 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk72f9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 91      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 46      6grp_total > 83      4grp_total > 16      6grp_highest_sub > 05      6grp_cgc > 88      6grp_high1_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2197
1 2 3 4 range
A 788.9
(-3)
P 		1380.7
(-1.2)
P 		1692.3
(-0.2)
P 		2735.7
(3)
P 		1947
B 765.3
(-3)
P 		1547.8
(0.2)
P 		1285.9
(-0.8)
P 		2217.3
(3)
P 		1452
C 710.2
(-3)
P 		1708.4
(-0.3)
P 		1543
(-0.7)
P 		2907.3
(3)
P 		2197
avr 754.8
(-3) 		1545.6
(-0.5) 		1507.1
(-0.6) 		2620.1
(3) 		1865

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2527
1 2 3 4 5 6 range
A 2860.3
(-2.3)
P 		2662.1
(-3)
P 		3096.7
(-1.4)
P 		2970.4
(-1.9)
P 		4286.9
(3)
P 		2783.4
(-2.6)
P 		1625
B 2153.7
(-2.9)
P 		2508.5
(-1.6)
P 		2139.8
(-3)
P 		3656.4
(2.8)
P 		3421.8
(1.9)
P 		3702.6
(3)
P 		1563
C 1951.7
(-3)
P 		2285.5
(-2.2)
P 		2735.6
(-1.1)
P 		2803.7
(-1)
P 		4478.6
(3)
P 		1993.1
(-2.9)
P 		2527
avr 2321.9
(-3) 		2485.4
(-2.4) 		2657.4
(-1.8) 		3143.5
(-0.2) 		4062.4
(3) 		2826.4
(-1.3) 		1741
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 303
1 2 3 4 range
A 287.2
(-3)
P 		299.7
(-2.5)
P 		442.4
(3)
P 		400.5
(1.4)
P 		155
B 244.1
(-3)
P 		382.3
(0.6)
P 		298
(-1.6)
P 		472.5
(3)
P 		228
C 235.1
(-3)
P 		491.2
(2.1)
P 		537.8
(3)
P 		490.2
(2.1)
P 		303
avr 255.5
(-3) 		391.1
(1.1) 		426.1
(2.1) 		454.4
(3) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 930
1 2 3 4 5 6 range
A 527.3
(-3)
P 		946.8
(0.3)
P 		674.4
(-1.8)
P 		753.4
(-1.2)
P 		1293.6
(3)
P 		677.7
(-1.8)
P 		766
B 749.4
(-1.4)
P 		835
(-0.8)
P 		519.6
(-3)
P 		968.5
(0.1)
P 		1390.2
(3)
P 		1380.7
(2.9)
P 		871
C 723.4
(-1.9)
P 		697.4
(-2.1)
P 		696.3
(-2.1)
P 		563.8
(-3)
P 		1449.2
(3)
P 		667.2
(-2.3)
P 		885
avr 666.7
(-2.7) 		826.4
(-1.4) 		630.1
(-3) 		761.9
(-1.9) 		1377.7
(3) 		908.5
(-0.8) 		748
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174675_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21861
Sequence Type Exemplar sequence
Representative Public ID CEK072F9R NCBI
Target Description CEK072F9R_rc /REP_DB=TREMBL Accession /GB=D66039 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk72f9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074468(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074468 NCBI Caenorhabditis elegans fatty Acid CoA Synthetase family member (acs-2) (acs-2) mRNA, complete cds. 11/11 None
F28F8.2.2 ENSEMBL cdna:known chromosome:CEL140:V:15569977:15572473:1 gene:F28F8.2 11/11 A 
F28F8.2.1 ENSEMBL cdna:known chromosome:CEL140:V:15569978:15572473:1 gene:F28F8.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15572035-15572447(+) 100.0 100.0
Public Domain and Genome References
Gene Title long-chain-fatty-acid-CoA ligase
Gene Symbol acs-2
Ensembl F28F8.2
Entrez Gene 3565680 Entrez gene
SwissProt O18693 EMBL-EBI
WormBase CE09756 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03012 Hypothetical protein F28F8.2 [Caenorhabditis elegans] ref|NP_506869.1| fatty Acid CoA Synthetase family member (acs-2) [Caenorhabditis elegans] 0.0
blast CAE71630 Hypothetical protein CBG18597 [Caenorhabditis briggsae] 0.0
blast AAV58866 Hypothetical protein F46E10.1c [Caenorhabditis elegans] ref|NP_001023939.1| F46E10.1c [Caenorhabditis elegans] 1.0E-175
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.9E-116
Sequence
Target Sequence 
>C. ELEGANS:174675_AT
gtaagagaggactacaaggttccaaagtacgtgctcatcaaaaaggagtccgaattccct
ctcacaatttccggaaaagtcaagaaattcgaaatccaaaagatttccaaagagctgttg
ggtctcggaactgtcaagtctcacttcacagaagctgtcgacaattaaaatcgggttgaa
catatttcctatattttaagttgccttttcgctgggatttccttgctttttacggtctaa
ccgctataattgtaatagtgacatatttaaccgggtttttatgtactcttccgtgtattt
gccatgtatttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAAGAGAGGACTACAAGGTTCCAA 433 461 24 Antisense
TATTTCCTATATTTTAAGTTGCCTT 500 661 206 Antisense
TAAGTTGCCTTTTCGCTGGGATTTC 384 637 220 Antisense
TTCGCTGGGATTTCCTTGCTTTTTA 534 697 231 Antisense
TTGCTTTTTACGGTCTAACCGCTAT 372 701 246 Antisense
ACGGTCTAACCGCTATAATTGTAAT 387 33 255 Antisense
TGTAATAGTGACATATTTAACCGGG 34 555 274 Antisense
GGTTTTTATGTACTCTTCCGTGTAT 142 507 297 Antisense
TATGTACTCTTCCGTGTATTTGCCA 380 655 303 Antisense
ACTCTTCCGTGTATTTGCCATGTAT 276 101 308 Antisense
TTCCGTGTATTTGCCATGTATTTAA 199 699 312 Antisense
Sequence Source Affymetrix Proprietary Database