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[174657_s_at]

Description CEK028B2R_rc /REP_DB=TREMBL Accession /GB=D33243 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk28b2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_high_8_8 > 46      6grp_highest_nonorm > 81      6grp_total > 81      4grp_total > 49      6grp_cgc > 74      6grp_high1_sub > 02_03_04_05      6grp_cgc_sub > 02_03_04_05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 575
1 2 3 4 range
A 59.2
(-3)
P 		622.9
(3)
P 		500.7
(1.7)
P 		56.8
(-3)
M 		566
B 69.5
(-3)
P 		456.8
(3)
P 		329.6
(1)
P 		79.8
(-2.8)
P 		387
C 47.7
(-3)
P 		479.1
(2.2)
P 		549.2
(3)
P 		80
(-2.6)
P 		502
avr 58.8
(-3) 		519.6
(3) 		459.8
(2.2) 		72.2
(-2.8) 		461

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 446
1 2 3 4 5 6 range
A 88.2
(-3)
P 		391.7
(3)
P 		283.4
(0.9)
P 		387.4
(2.9)
P 		354.8
(2.3)
P 		91.6
(-2.9)
P 		304
B 118.8
(-3)
P 		386.5
(2.9)
P 		225.7
(-0.6)
P 		390.8
(3)
P 		327.2
(1.6)
P 		121.3
(-2.9)
P 		272
C 108.6
(-3)
P 		333.2
(0.2)
P 		241.1
(-1.1)
P 		534
(3)
P 		240.9
(-1.1)
P 		107
(-3)
P 		427
avr 105.2
(-3) 		370.5
(1.8) 		250.1
(-0.4) 		437.4
(3) 		307.6
(0.7) 		106.6
(-3) 		332
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 21.5
(-2.4)
P 		135.2
(3)
P 		130.9
(2.8)
P 		8.3
(-3)
M 		127
B 22.2
(-2.7)
P 		112.8
(3)
P 		76.4
(0.7)
P 		17
(-3)
P 		96
C 15.8
(-2.9)
P 		137.7
(1.2)
P 		191.4
(3)
P 		13.5
(-3)
P 		178
avr 19.8
(-2.7) 		128.6
(2.8) 		132.9
(3) 		12.9
(-3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 16.3
(-3)
P 		139.3
(3)
P 		61.7
(-0.8)
P 		98.3
(1)
P 		107.1
(1.4)
P 		22.3
(-2.7)
P 		123
B 41.3
(-3)
P 		128.6
(2.7)
P 		54.8
(-2.1)
P 		103.5
(1.1)
P 		132.9
(3)
P 		45.2
(-2.7)
P 		92
C 40.2
(-2.6)
P 		101.7
(2.5)
P 		61.4
(-0.9)
P 		107.4
(3)
P 		77.9
(0.5)
P 		35.8
(-3)
P 		72
avr 32.6
(-3) 		123.2
(3) 		59.3
(-1.2) 		103.1
(1.7) 		106
(1.9) 		34.4
(-2.9) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174657_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20225
Sequence Type Exemplar sequence
Representative Public ID CEK028B2R NCBI
Target Description CEK028B2R_rc /REP_DB=TREMBL Accession /GB=D33243 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk28b2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059272(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059272 NCBI Caenorhabditis elegans abnormal CHEmotaxis family member (che-14) (che-14) mRNA, complete cds. 11/11 A 
GENEFINDER00000038427 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5752320:5762255:1 11/11 None
SNAP00000038419 ENSEMBL cdna:SNAP chromosome:CEL140:I:5757114:5762255:1 11/11 None
F56H1.1 ENSEMBL cdna:known chromosome:CEL140:I:5754861:5762427:1 gene:F56H1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5761563-5762404(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol che-14
Ensembl F56H1.1
Entrez Gene 172240 Entrez gene
SwissProt O76372 EMBL-EBI
WormBase CE28468 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC19198 Abnormal chemotaxis protein 14 [Caenorhabditis elegans] ref|NP_491673.1| abnormal CHEmotaxis family member (che-14) [Caenorhabditis elegans] emb|CAB90211.1| CHE-14 protein [Caenorhabditis elegans] 0.0
blast CAE67389 Hypothetical protein CBG12874 [Caenorhabditis briggsae] 0.0
blast AAY44003 Hypothetical protein F56H1.6 [Caenorhabditis elegans] ref|NP_001021512.1| F56H1.6 [Caenorhabditis elegans] 0.0
blast NP_505396 disPaTchED family member (ptd-2) [Caenorhabditis elegans] gb|AAG24025.2| Dispatched family protein 2 [Caenorhabditis elegans] 9.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR002085 EMBL-EBI Zinc-containing alcohol dehydrogenase superfamily 1.2E-36
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491673.1 14 19-41,267-289,321-343,347-369,376-398,413-435,448-470,485-504,509-531,743-765,772-794,798-820,840-862,866-888
F56H1.1 14 19-41,267-289,321-343,347-369,376-398,413-435,448-470,485-504,509-531,743-765,772-794,798-820,840-862,866-888
GENEFINDER00000038427 13 579-601,633-655,659-681,688-710,725-747,760-782,797-816,821-843,1055-1077,1084-1106,1110-1132,1152-1174,1178-1200
SNAP00000038419 8 280-302,307-329,357-379,592-614,621-643,647-669,689-711,715-737
Sequence
Target Sequence 
>C. ELEGANS:174657_S_AT
gtaggacctgttacattggctgcattcacttcatttttagctggagcttcaatgttacca
gcgttgactcatgcgttctatcaggtggtgtgttccttgttgtaatatccataacttcat
ggacattttcaacatttttctatcttccaatgctctctctaacacttcctaaccaatctg
gaatatgcccttattgtgaaaaaaccaatctaatgcatttatcacctcggagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGGACCTGTTACATTGGCTGCAT 594 455 62 Antisense
ACATTGGCTGCATTCACTTCATTTT 569 103 74 Antisense
TCACTTCATTTTTAGCTGGAGCTTC 185 627 87 Antisense
GGAGCTTCAATGTTACCAGCGTTGA 409 521 104 Antisense
CGTTGACTCATGCGTTCTATCAGGT 147 247 123 Antisense
TGGTGTGTTCCTTGTTGTAATATCC 641 551 147 Antisense
AACTTCATGGACATTTTCAACATTT 473 139 174 Antisense
CATTTTTCTATCTTCCAATGCTCTC 303 215 194 Antisense
TCTCTAACACTTCCTAACCAATCTG 161 613 217 Antisense
TAACCAATCTGGAATATGCCCTTAT 667 637 231 Antisense
CTAATGCATTTATCACCTCGGAGTT 214 225 271 Antisense
Sequence Source Affymetrix Proprietary Database