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[174646_at]

Description CEK023F9R_rc /REP_DB=TREMBL Accession /GB=D32935 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk23f9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 40      6grp_total > 05      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 498
1 2 3 4 range
A 928.7
(3)
P 		600.1
(-1)
P 		430.8
(-3)
P 		490.1
(-2.3)
P 		498
B 773.5
(3)
P 		691.7
(0.7)
P 		562.4
(-3)
P 		580.8
(-2.5)
P 		211
C 736.7
(3)
P 		670.6
(1.2)
P 		513.7
(-3)
P 		535.9
(-2.4)
P 		223
avr 813
(3) 		654.1
(-0.1) 		502.3
(-3) 		535.6
(-2.4) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 750
1 2 3 4 5 6 range
A 903.5
(3)
P 		727
(1.3)
P 		420.3
(-1.7)
P 		315.7
(-2.7)
P 		554.8
(-0.4)
P 		283.9
(-3)
P 		620
B 1034.2
(3)
P 		836.8
(1.2)
P 		549.8
(-1.4)
P 		516.7
(-1.7)
P 		518.7
(-1.7)
P 		370.5
(-3)
P 		664
C 875
(3)
P 		769.7
(1.9)
P 		403.6
(-2.1)
P 		408.5
(-2)
P 		493.1
(-1.1)
P 		319.7
(-3)
P 		555
avr 937.6
(3) 		777.8
(1.4) 		457.9
(-1.7) 		413.6
(-2.1) 		522.2
(-1.1) 		324.7
(-3) 		613
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 338.1
(3)
P 		130.3
(-1.7)
P 		112.6
(-2.1)
P 		71.7
(-3)
P 		266
B 246.7
(3)
P 		170.8
(-0.7)
P 		130.3
(-2.7)
P 		123.8
(-3)
P 		123
C 243.9
(3)
P 		192.8
(1)
P 		179
(0.5)
P 		90.4
(-3)
P 		154
avr 276.2
(3) 		164.6
(-0.7) 		140.6
(-1.5) 		95.3
(-3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 166.6
(0.1)
P 		258.6
(3)
P 		91.5
(-2.3)
P 		80.1
(-2.7)
P 		167.4
(0.1)
P 		69.1
(-3)
P 		190
B 359.8
(3)
P 		278.5
(0.8)
P 		133.5
(-3)
P 		136.8
(-2.9)
P 		210.8
(-1)
P 		138.2
(-2.9)
P 		226
C 324.3
(3)
P 		234.9
(0.8)
P 		102.7
(-2.5)
P 		82.1
(-3)
P 		159.6
(-1.1)
P 		107
(-2.4)
P 		242
avr 283.6
(3) 		257.3
(2.1) 		109.2
(-2.7) 		99.7
(-3) 		179.3
(-0.4) 		104.8
(-2.8) 		184
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174646_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21112
Sequence Type Exemplar sequence
Representative Public ID CEK023F9R NCBI
Target Description CEK023F9R_rc /REP_DB=TREMBL Accession /GB=D32935 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk23f9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027735(11), NM_001027736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027735 NCBI Caenorhabditis elegans mammalian FE65 Homolog family member (feh-1) (feh-1) mRNA, complete cds. 11/11 None
NM_001027736 NCBI Caenorhabditis elegans mammalian FE65 Homolog family member (feh-1) (feh-1) mRNA, complete cds. 11/11 None
Y54F10AM.2b ENSEMBL cdna:known chromosome:CEL140:III:2549916:2560662:1 gene:Y54F10AM.2 11/11 A 
Y54F10AM.2c ENSEMBL cdna:known chromosome:CEL140:III:2554948:2560657:1 gene:Y54F10AM.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000034926 3/11 Cross Hyb Matching Probes None
SNAP00000034924 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175373 3 2 NM_001027735 174646_at (11)
NM_001027736 174646_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2560321-2560641(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54F10AM.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84831 Mammalian fe65 homolog protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022906.1| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
blast AAK29940 Mammalian fe65 homolog protein 1, isoform a [Caenorhabditis elegans] ref|NP_497578.3| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
blast CAE65009 Hypothetical protein CBG09847 [Caenorhabditis briggsae] 0.0
blast CAC87812 FEH-1 protein [Caenorhabditis elegans] 0.0
blast AAN84832 Mammalian fe65 homolog protein 1, isoform c [Caenorhabditis elegans] ref|NP_001022907.1| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Sequence
Target Sequence 
>C. ELEGANS:174646_AT
gcgacgcttttggttcggtgacgtcacgtatggtaccttctaggtcggcacaacgacttt
gaattcttcatttttcttcggttctttaattttctgtatttttgatggatttttctcata
tttttttctctcatactctgttctgctcatcccaaatatgctttcaaaccaaaagtttac
cccggcccctcacttggcaatattcttcaatccaccgatacatttttacttgtacaatcc
ctctttctgtgtagctttttcaacatctgtgacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGACGCTTTTGGTTCGGTGACGTC 421 289 23 Antisense
GGTGACGTCACGTATGGTACCTTCT 334 499 39 Antisense
GTACCTTCTAGGTCGGCACAACGAC 518 293 55 Antisense
GGCACAACGACTTTGAATTCTTCAT 537 535 69 Antisense
TGTTCTGCTCATCCCAAATATGCTT 249 563 161 Antisense
GGCCCCTCACTTGGCAATATTCTTC 96 543 206 Antisense
GCAATATTCTTCAATCCACCGATAC 80 323 219 Antisense
CAATCCACCGATACATTTTTACTTG 35 181 230 Antisense
CATTTTTACTTGTACAATCCCTCTT 218 215 243 Antisense
CCTCTTTCTGTGTAGCTTTTTCAAC 677 267 262 Antisense
GTAGCTTTTTCAACATCTGTGACAA 437 457 273 Antisense
Sequence Source Affymetrix Proprietary Database