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[174639_at] serine/threonine kinase (KIN1/SNF1/Nim1 subfamily)

Description CEK041F2R_rc /REP_DB=TREMBL Accession /GB=D34142 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41f2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 46      6grp_highest_sub > 01_02      6grp_cgc > 23      6grp_high1_sub > 01_02      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 422
1 2 3 4 range
A 676.9
(3)
P 		353
(-1.7)
P 		264.3
(-3)
P 		377.5
(-1.4)
P 		413
B 644
(3)
P 		424.5
(-0.9)
P 		303
(-3)
P 		333.6
(-2.5)
P 		341
C 686.7
(3)
P 		446.5
(-1)
P 		329.7
(-3)
P 		381.1
(-2.1)
P 		357
avr 669.2
(3) 		408
(-1.2) 		299
(-3) 		364.1
(-1.9) 		370

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 477
1 2 3 4 5 6 range
A 590.1
(3)
P 		487.4
(1.6)
P 		239.2
(-1.7)
P 		142.8
(-3)
P 		271.5
(-1.3)
P 		224.1
(-1.9)
P 		447
B 619.9
(3)
P 		536.4
(1.8)
P 		242.9
(-2.6)
P 		304.9
(-1.7)
P 		260.9
(-2.3)
P 		217.1
(-3)
P 		403
C 475
(1.1)
P 		607.9
(3)
P 		323.5
(-1.1)
P 		276.8
(-1.8)
P 		194.6
(-3)
P 		255.8
(-2.1)
P 		413
avr 561.7
(3) 		543.9
(2.7) 		268.5
(-2.3) 		241.5
(-2.8) 		242.3
(-2.8) 		232.3
(-3) 		329
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 246.5
(3)
P 		76.6
(-2.3)
P 		69.1
(-2.6)
P 		55.3
(-3)
P 		191
B 205.4
(3)
P 		104.9
(-1.5)
P 		70.2
(-3)
P 		71.1
(-3)
P 		135
C 227.3
(3)
P 		128.4
(-0.6)
P 		114.9
(-1.1)
P 		64.3
(-3)
P 		163
avr 226.4
(3) 		103.3
(-1.5) 		84.7
(-2.2) 		63.6
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 108.8
(0.2)
P 		173.3
(3)
P 		52.1
(-2.3)
P 		36.2
(-3)
P 		81.9
(-1)
P 		54.6
(-2.2)
P 		137
B 215.7
(3)
P 		178.5
(1.6)
P 		59
(-3)
P 		80.8
(-2.2)
P 		106
(-1.2)
P 		81
(-2.2)
P 		157
C 176.1
(2.6)
P 		185.5
(3)
P 		82.3
(-1.8)
P 		55.7
(-3)
P 		63
(-2.7)
P 		85.6
(-1.6)
P 		130
avr 166.9
(2.4) 		179.1
(3) 		64.5
(-2.7) 		57.6
(-3) 		83.6
(-1.7) 		73.7
(-2.2) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174639_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22356
Sequence Type Exemplar sequence
Representative Public ID CEK041F2R NCBI
Target Description CEK041F2R_rc /REP_DB=TREMBL Accession /GB=D34142 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41f2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077852(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077852 NCBI Caenorhabditis elegans Synapses of Amphids Defective family member (sad-1) (sad-1) mRNA, complete cds. 11/11 A 
F15A2.6 ENSEMBL cdna:known chromosome:CEL140:X:13487483:13500569:-1 gene:F15A2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13487492-13487863(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase (KIN1/SNF1/Nim1 subfamily)
Gene Symbol sad-1
Ensembl F15A2.6
Entrez Gene 181471 Entrez gene
SwissProt Q19469 EMBL-EBI
WormBase CE28218 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.4766.1.S1_S_AT similar to BR serine/threonine-protein kinase 1 (SAD1 kinase) (SAD1A) cfa
DROSOPHILA_2:1634134_AT dm
DROSOPHILA_2:1623313_AT dm
DROSGENOME1:148833_AT dm
U133_X3P:HS2.182081.1.S1_3P_X_AT BR serine/threonine kinase 1 hs
U133_X3P:HS2.182081.1.S1_3P_S_AT BR serine/threonine kinase 1 hs
U133_X3P:HS2.182081.1.S1_3P_A_AT BR serine/threonine kinase 1 hs
U133_X3P:1552504_3P_A_AT BR serine/threonine kinase 1 hs
HG-U133_PLUS_2:1552504_A_AT BR serine/threonine kinase 1 hs
HG-U95E:67204_AT BR serine/threonine kinase 1 hs
MOE430B:1439892_AT BR serine/threonine kinase 1 mm
MOUSE430_2:1439892_AT BR serine/threonine kinase 1 mm
MG-U74BV2:105888_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
MU19KSUBB:TC30057_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
MU19KSUBB:TC30057_G_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
30424 axon inferred from direct assay QuickGO AmiGO
30425 dendrite inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from sequence similarity QuickGO AmiGO
5524 ATP binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94127 Hypothetical protein F15A2.6 [Caenorhabditis elegans] ref|NP_510253.1| Synapses of Amphids Defective family member (sad-1) [Caenorhabditis elegans] gb|AAG50270.1| serine/threonine kinase SAD-1 [Caenorhabditis elegans] 0.0
blast CAE63138 Hypothetical protein CBG07440 [Caenorhabditis briggsae] 0.0
blast AAF49569 CG6114-PA [Drosophila melanogaster] ref|NP_648814.3| CG6114-PA [Drosophila melanogaster] 1.0E-162
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-69
hanks 2.2.4 CaMK Group; CaMK II KIN1/SNF1/Nim1; p78 5.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.6E-95
Sequence
Target Sequence 
>C. ELEGANS:174639_AT
aatgtacagatctttttgaaaaactattttcctccactttcatcatggtttcgccctgct
cttattttgtagaaaacgattatttctgcagttcatttctaattgccaaacattgaaaca
tgcattccctgttctcacattccatttttttccccaatttgtgtctagtttgaatctcag
tcttttttaccaatttttgtcctttttgctttgttcctcataccaaacttcattgtactg
tcttcattatctcttttttcaattttattttaacggactgttctggtttttcctcggttc
tctgtaaaacttcatttaactatttccatcggatgccattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTACAGATCTTTTTGAAAAACT 404 161 13 Antisense
TATTTTCCTCCACTTTCATCATGGT 325 663 37 Antisense
TTTCCTCCACTTTCATCATGGTTTC 72 671 40 Antisense
TCCTCCACTTTCATCATGGTTTCGC 23 593 42 Antisense
TTCGCCCTGCTCTTATTTTGTAGAA 363 697 62 Antisense
GCCCTGCTCTTATTTTGTAGAAAAC 527 285 65 Antisense
TGAAACATGCATTCCCTGTTCTCAC 463 573 126 Antisense
GTTTGAATCTCAGTCTTTTTTACCA 252 451 180 Antisense
TTGAATCTCAGTCTTTTTTACCAAT 59 703 182 Antisense
TCATTTAACTATTTCCATCGGATGC 637 619 324 Antisense
TAACTATTTCCATCGGATGCCATTT 557 637 329 Antisense
Sequence Source Affymetrix Proprietary Database