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[174638_at]

Description CEK015G4R_rc /REP_DB=TREMBL Accession /GB=D27624 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk15g4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 49      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 86      6grp_total > 48      4grp_total > 55      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 869
1 2 3 4 range
A 1276
(3)
P 		534.6
(-2.1)
P 		712.7
(-0.9)
P 		406.6
(-3)
P 		869
B 1127.5
(3)
P 		744.2
(-1.8)
P 		830.3
(-0.8)
P 		652.9
(-3)
P 		475
C 1141.8
(3)
P 		763.7
(-1)
P 		775.3
(-0.9)
P 		580.7
(-3)
P 		561
avr 1181.8
(3) 		680.8
(-1.7) 		772.8
(-0.9) 		546.7
(-3) 		635

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 714
1 2 3 4 5 6 range
A 1285.4
(3)
P 		1140.7
(1.2)
P 		796.1
(-3)
P 		931.7
(-1.3)
P 		1235.5
(2.4)
P 		1080
(0.5)
P 		489
B 886.3
(-1)
P 		835.6
(-1.4)
P 		645.6
(-3)
P 		1359.2
(3)
P 		894.6
(-0.9)
P 		1145.2
(1.2)
P 		714
C 1018
(3)
P 		1009.3
(2.8)
P 		954.9
(1.9)
P 		948.6
(1.7)
P 		686
(-3)
P 		958.5
(1.9)
P 		332
avr 1063.2
(2.6) 		995.2
(1.2) 		798.9
(-3) 		1079.8
(3) 		938.7
(-0) 		1061.2
(2.6) 		281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 405
1 2 3 4 range
A 464.6
(3)
P 		116.1
(-2.2)
P 		186.3
(-1.1)
P 		59.5
(-3)
P 		405
B 359.6
(3)
P 		183.8
(-1.8)
P 		192.4
(-1.5)
P 		139.1
(-3)
P 		221
C 378
(3)
P 		219.6
(-0.4)
P 		270.2
(0.7)
P 		97.9
(-3)
P 		280
avr 400.7
(3) 		173.2
(-1.5) 		216.3
(-0.7) 		98.8
(-3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 270
1 2 3 4 5 6 range
A 236.9
(-1.4)
P 		405.7
(3)
P 		173.4
(-3)
P 		236.3
(-1.4)
P 		372.8
(2.2)
P 		263
(-0.7)
P 		232
B 308.4
(0.4)
P 		278.1
(-0.3)
P 		156.8
(-3)
P 		360
(1.5)
P 		363.5
(1.6)
P 		427
(3)
P 		270
C 377.3
(3)
P 		308
(0.8)
P 		243
(-1.3)
P 		190.8
(-3)
P 		222
(-2)
P 		320.9
(1.2)
P 		187
avr 307.5
(1.8) 		330.6
(2.7) 		191.1
(-3) 		262.4
(-0.1) 		319.4
(2.3) 		337
(3) 		146
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174638_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21351
Sequence Type Exemplar sequence
Representative Public ID CEK015G4R NCBI
Target Description CEK015G4R_rc /REP_DB=TREMBL Accession /GB=D27624 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk15g4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068938(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068938 NCBI Caenorhabditis elegans neuRonal IGCAM family member (rig-4) (rig-4) mRNA, complete cds. 11/11 None
Y42H9B.2 ENSEMBL cdna:known chromosome:CEL140:IV:7991391:8004027:-1 gene:Y42H9B.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7991427-7991782(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rig-4
Ensembl Y42H9B.2
Entrez Gene 177597 Entrez gene
SwissProt Q9N3X8 EMBL-EBI
WormBase CE33870 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7692.1.S1_S_AT similar to sidekick 2 cfa
DROSOPHILA_2:1628319_S_AT sidekick dm
DROSGENOME1:154090_AT sidekick dm
CHICKEN:GGA.118.1.S1_AT sidekick homolog 2 (chicken) gga
HG-U133_PLUS_2:233585_AT sidekick homolog 2 (chicken) hs
HG-U133B:233585_AT sidekick homolog 2 (chicken) hs
HG-U133_PLUS_2:232061_AT sidekick homolog 2 (chicken) hs
HG-U133B:232061_AT sidekick homolog 2 (chicken) hs
HG-U133_PLUS_2:220628_S_AT sidekick homolog 2 (chicken) hs
HG-U133A:220628_S_AT sidekick homolog 2 (chicken) hs
HG-U133A_2:220628_S_AT sidekick homolog 2 (chicken) hs
HU35KSUBA:AA452721_AT sidekick homolog 2 (chicken) hs
HU35KSUBC:RC_N23651_AT sidekick homolog 2 (chicken) hs
U133_X3P:G9506620_3P_A_AT sidekick homolog 2 (chicken) hs
U133_X3P:HS.43410.0.A1_3P_AT sidekick homolog 2 (chicken) hs
U133_X3P:HS.125490.2.S1_3P_AT sidekick homolog 2 (chicken) hs
U133_X3P:HS.125490.1.S1_3P_AT sidekick homolog 2 (chicken) hs
HG-U133_PLUS_2:242064_AT sidekick homolog 2 (chicken) hs
HG-U133B:242064_AT sidekick homolog 2 (chicken) hs
HG-U95B:54276_AT sidekick homolog 2 (chicken) hs
HG-U95E:81862_AT sidekick homolog 2 (chicken) hs
MG-U74BV2:105828_AT sidekick homolog 2 (chicken) mm
MOE430B:1430425_AT sidekick homolog 2 (chicken) mm
MOUSE430_2:1430425_AT sidekick homolog 2 (chicken) mm
MOE430B:1457333_AT sidekick homolog 2 (chicken) mm
MOUSE430_2:1457333_AT sidekick homolog 2 (chicken) mm
MG-U74CV2:138166_AT MKIAA1514 protein mm
MOE430B:1447445_AT MKIAA1514 protein mm
MOUSE430_2:1447445_AT MKIAA1514 protein mm
MU19KSUBC:TC38342_AT MKIAA1514 protein mm
RAE230B:1381475_AT sidekick homolog 2 (chicken) (predicted) rn
RAT230_2:1381475_AT sidekick homolog 2 (chicken) (predicted) rn
RAE230B:1379016_AT Sidekick homolog 2 (chicken) (predicted) rn
RAT230_2:1379016_AT Sidekick homolog 2 (chicken) (predicted) rn
RAE230B:1397981_AT Sidekick homolog 2 (chicken) (predicted) rn
RAT230_2:1397981_AT Sidekick homolog 2 (chicken) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60566 Neuronal igcam protein 4 [Caenorhabditis elegans] ref|NP_501339.2| neuRonal IGCAM family member (rig-4) [Caenorhabditis elegans] 0.0
blast CAE70924 Hypothetical protein CBG17724 [Caenorhabditis briggsae] 0.0
blast AAN09028 CG5227-PC, isoform C [Drosophila melanogaster] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 6.3E-18
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.6E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.3E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.1E-16
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.7E-20
Pfam IPR003961 EMBL-EBI Fibronectin, type III 9.7E-15
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.1E-13
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.0E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.0E-8
Pfam IPR003961 EMBL-EBI Fibronectin, type III 9.3E-11
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.8E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501339.2 2 5-24,2019-2041
Y42H9B.2 2 5-24,2019-2041
Sequence
Target Sequence 
>C. ELEGANS:174638_AT
atctgtctgtgacttgttccatttctgtctcaattttgttgttcaaaattcttgccgttt
tctttttttttcttcacaaatggtttttttttctgtaccattcggatacttaatttctct
cccttttcctgtaccggtgacatacgccaagttgtccacttaatttccctgaaacataat
tatatctggccgattttctctaaaacgggttttcataatgatgagttttttgtctttttc
cccctcattttcagccccaattttagaatctcgtttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTGTCTGTGACTTGTTCCATTTC 19 35 22 Antisense
GTTCCATTTCTGTCTCAATTTTGTT 528 441 37 Antisense
TGTTCAAAATTCTTGCCGTTTTCTT 425 561 61 Antisense
TTTTTTCTGTACCATTCGGATACTT 103 675 108 Antisense
TCGGATACTTAATTTCTCTCCCTTT 397 605 123 Antisense
CTTTTCCTGTACCGGTGACATACGC 402 221 144 Antisense
GTGACATACGCCAAGTTGTCCACTT 591 481 158 Antisense
TTGTCCACTTAATTTCCCTGAAACA 283 695 173 Antisense
ATATCTGGCCGATTTTCTCTAAAAC 271 21 203 Antisense
TAATGATGAGTTTTTTGTCTTTTTC 624 635 237 Antisense
GCCCCAATTTTAGAATCTCGTTTAT 427 285 275 Antisense
Sequence Source Affymetrix Proprietary Database