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[174626_at]

Description CEK042E3R_rc /REP_DB=TREMBL Accession /GB=D34200 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk42e3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 37      4grp_high_8_8 > 02      6grp_highest_nonorm > 0      6grp_total > 17      4grp_total > 06      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1983
1 2 3 4 range
A 2124
(3)
P 		1092.4
(-3)
P 		1455
(-0.9)
P 		2072.6
(2.7)
P 		1032
B 2404.2
(0.5)
P 		1457.7
(-3)
P 		1461.2
(-3)
P 		3075.5
(3)
P 		1618
C 2006.1
(0.6)
P 		1455.7
(-3)
P 		1608.3
(-2)
P 		2378.3
(3)
P 		923
avr 2178.1
(1.3) 		1335.3
(-3) 		1508.2
(-2.1) 		2508.8
(3) 		1174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1483
1 2 3 4 5 6 range
A 2162.7
(3)
P 		1691.8
(0.2)
P 		1325.2
(-2)
P 		1156.4
(-3)
P 		1412.6
(-1.5)
P 		1298
(-2.2)
P 		1006
B 2446.8
(3)
P 		1805.8
(0.4)
P 		1487.6
(-0.9)
P 		1487.6
(-0.9)
P 		963.7
(-3)
P 		1365.6
(-1.4)
P 		1483
C 2157.7
(3)
P 		1580.4
(-0.9)
P 		1316.6
(-2.7)
P 		1271.2
(-3)
P 		1290.4
(-2.9)
P 		1657.5
(-0.4)
P 		887
avr 2255.7
(3) 		1692.7
(-0.3) 		1376.5
(-2.1) 		1305.1
(-2.5) 		1222.2
(-3) 		1440.4
(-1.7) 		1034
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 536
1 2 3 4 range
A 773.3
(3)
P 		237.1
(-3)
P 		380.4
(-1.4)
P 		303.4
(-2.3)
P 		536
B 766.7
(3)
P 		360
(-2.7)
P 		338.6
(-3)
P 		655.4
(1.4)
P 		428
C 664.1
(3)
P 		418.5
(-2.6)
P 		560.5
(0.6)
P 		401
(-3)
P 		263
avr 734.7
(3) 		338.5
(-3) 		426.5
(-1.7) 		453.3
(-1.3) 		396

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 596
1 2 3 4 5 6 range
A 398.7
(-0.9)
P 		601.7
(3)
P 		288.6
(-3)
P 		293.3
(-2.9)
P 		426.3
(-0.4)
P 		316
(-2.5)
P 		313
B 851.4
(3)
P 		601.1
(-0.1)
P 		361.2
(-3)
P 		394
(-2.6)
P 		391.5
(-2.6)
P 		509.2
(-1.2)
P 		490
C 799.8
(3)
P 		482.2
(-0.5)
P 		335.1
(-2.1)
P 		255.6
(-3)
P 		417.6
(-1.2)
P 		554.9
(0.3)
P 		544
avr 683.3
(3) 		561.7
(1) 		328.3
(-2.8) 		314.3
(-3) 		411.8
(-1.4) 		460
(-0.6) 		369
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174626_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21345
Sequence Type Exemplar sequence
Representative Public ID CEK042E3R NCBI
Target Description CEK042E3R_rc /REP_DB=TREMBL Accession /GB=D34200 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk42e3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068740 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-158) (tag-158) mRNA, complete cds. 11/11 None
R13A1.2 ENSEMBL cdna:known chromosome:CEL140:IV:7213701:7217972:1 gene:R13A1.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7217568-7217968(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-158
Ensembl R13A1.2
Entrez Gene 177495 Entrez gene
SwissProt Q21977 EMBL-EBI
WormBase CE04838 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_501141 Temporarily Assigned Gene name family member (tag-158) [Caenorhabditis elegans] gb|AAA81476.1| Temporarily assigned gene name protein 158 [Caenorhabditis elegans] 0.0
blast CAE61836 Hypothetical protein CBG05808 [Caenorhabditis briggsae] 0.0
blast CAC80545 putative Na-K-Cl cotransporter [Meloidogyne incognita] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-14
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501141.1 10 36-58,70-92,131-153,166-188,192-214,327-349,364-386,464-486,490-512,525-547
R13A1.2 10 36-58,70-92,131-153,166-188,192-214,327-349,364-386,464-486,490-512,525-547
Sequence
Target Sequence 
>C. ELEGANS:174626_AT
cctccgtactctcgtcggaatatgatgaacggcattttatatattcgtttaggtagttcc
tgatttgttttttttgttttcttgaaaattgattgatgttcaaattttttttgaataatt
tcttatcaactttgtagattctttccatcctaaacattgccgatgttagtaccgtaacct
accgtaagtcgtgttttcctccctgtggtcattcactgtttttatgtcaaaggagtctgt
ctgactttacttctatcattcaccatccccttcagtatcatatcaaccccgatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTCCGTACTCTCGTCGGAATATGA 404 267 53 Antisense
CGGCATTTTATATATTCGTTTAGGT 46 1 82 Antisense
TCGTTTAGGTAGTTCCTGATTTGTT 643 603 97 Antisense
CAACTTTGTAGATTCTTTCCATCCT 543 187 179 Antisense
TTCTTTCCATCCTAAACATTGCCGA 673 689 191 Antisense
TGCCGATGTTAGTACCGTAACCTAC 642 585 210 Antisense
TCCCTGTGGTCATTCACTGTTTTTA 630 597 252 Antisense
GGTCATTCACTGTTTTTATGTCAAA 306 503 259 Antisense
AAGGAGTCTGTCTGACTTTACTTCT 458 159 282 Antisense
GACTTTACTTCTATCATTCACCATC 559 371 295 Antisense
TTCAGTATCATATCAACCCCGATCC 554 687 323 Antisense
Sequence Source Affymetrix Proprietary Database