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[174625_at]

Description CEK014D4R_rc /REP_DB=TREMBL Accession /GB=D27333 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk14d4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 38      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 01      6grp_total > 38      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3802
1 2 3 4 range
A 2219.4
(-2)
P 		1625.4
(-3)
P 		1981.1
(-2.4)
P 		5370.1
(3)
P 		3745
B 2466
(-0.1)
P 		1862.7
(-2.1)
P 		1568.5
(-3)
P 		3433
(3)
P 		1865
C 1864.1
(-2.3)
P 		1923.8
(-2.1)
P 		1607.2
(-3)
P 		3771.9
(3)
P 		2165
avr 2183.2
(-1.9) 		1804
(-2.8) 		1718.9
(-3) 		4191.7
(3) 		2473

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2527
1 2 3 4 5 6 range
A 3434.6
(3)
P 		1498.4
(-1.6)
P 		907.6
(-3)
P 		924.1
(-3)
P 		1409.8
(-1.8)
P 		2217.7
(0.1)
P 		2527
B 2940.4
(3)
P 		1443.3
(-1.8)
P 		1060.1
(-3)
P 		2117.4
(0.4)
P 		1382.5
(-2)
P 		2103.3
(0.3)
P 		1880
C 2588.1
(3)
P 		1437.9
(-1.4)
P 		1557.6
(-1)
P 		1027
(-3)
P 		1289.7
(-2)
P 		2520.2
(2.7)
P 		1561
avr 2987.7
(3) 		1459.9
(-2.1) 		1175.1
(-3) 		1356.2
(-2.4) 		1360.7
(-2.4) 		2280.4
(0.7) 		1813
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 455
1 2 3 4 range
A 808.1
(3)
P 		352.8
(-3)
P 		517.9
(-0.8)
P 		786.1
(2.7)
P 		455
B 786.4
(3)
P 		460
(-1.6)
P 		363.5
(-3)
P 		731.6
(2.2)
P 		423
C 617.1
(1.6)
P 		553.1
(-3)
P 		560.2
(-2.5)
P 		636
(3)
P 		83
avr 737.2
(3) 		455.3
(-3) 		480.5
(-2.5) 		717.9
(2.6) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 825
1 2 3 4 5 6 range
A 633.1
(3)
P 		532.9
(1.6)
P 		197.7
(-3)
P 		234.4
(-2.5)
P 		425.4
(0.1)
P 		539.9
(1.7)
P 		435
B 1023.1
(3)
P 		480.4
(-1.3)
P 		257.4
(-3)
P 		560.8
(-0.6)
P 		561.7
(-0.6)
P 		784.3
(1.1)
P 		766
C 959.3
(3)
P 		438.7
(-1.1)
P 		396.4
(-1.5)
P 		206.5
(-3)
P 		417.3
(-1.3)
P 		843.6
(2.1)
P 		753
avr 871.8
(3) 		484
(-1) 		283.8
(-3) 		333.9
(-2.5) 		468.1
(-1.1) 		722.6
(1.5) 		588
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174625_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21693
Sequence Type Exemplar sequence
Representative Public ID CEK014D4R NCBI
Target Description CEK014D4R_rc /REP_DB=TREMBL Accession /GB=D27333 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk14d4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171484(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171484 NCBI Caenorhabditis elegans IMportin Beta family member (imb-4) (imb-4) mRNA, complete cds. 11/11 None
ZK742.1a.3 ENSEMBL cdna:known chromosome:CEL140:V:7807654:7817213:1 gene:ZK742.1 11/11 None
ZK742.1a.1 ENSEMBL cdna:known chromosome:CEL140:V:7807656:7817213:1 gene:ZK742.1 11/11 A 
ZK742.1a.2 ENSEMBL cdna:known chromosome:CEL140:V:7807671:7817213:1 gene:ZK742.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179160 2 1 NM_171484 174625_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7816784-7817204(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK742.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04981 Importin beta family protein 4, isoform a [Caenorhabditis elegans] ref|NP_741567.1| IMportin Beta family member (imb-4) [Caenorhabditis elegans] 0.0
blast CAE73178 Hypothetical protein CBG20576 [Caenorhabditis briggsae] 0.0
blast XP_531839 PREDICTED: similar to exportin 1, CRM1 homolog isoform 1 [Canis familiaris] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001494 EMBL-EBI Importin-beta, N-terminal 2.6E-16
Sequence
Target Sequence 
>C. ELEGANS:174625_AT
ttttatttctctaccagttactgtgtagctcccctcaaccggatctcggaacccctttct
ctcccactacttgataactggaatcttctctggacctacttccattcatcatttatcata
atttctgtattcatccgtgcgccccctatttttctctgtgaaccattttggcaaattttc
aaatttcttcaaaaatttgaacatgttcattactcttattttttgtaattgccggaccca
atttttttgaatccttctgtcaattgtcactcttcctcaattcatcattcttgttgttca
ttgattcattcaattattctttattattttatcatacatttttattttacccccaccacc
agcaaatacagttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTATTTCTCTACCAGTTACTGTG 171 675 22 Antisense
CTACCAGTTACTGTGTAGCTCCCCT 264 145 32 Antisense
TTCTCTCCCACTACTTGATAACTGG 153 693 78 Antisense
TAACTGGAATCTTCTCTGGACCTAC 546 637 96 Antisense
CTGGACCTACTTCCATTCATCATTT 501 241 111 Antisense
ATAATTTCTGTATTCATCCGTGCGC 7 27 139 Antisense
CCCCTATTTTTCTCTGTGAACCATT 98 255 164 Antisense
GACCCAATTTTTTTGAATCCTTCTG 284 371 256 Antisense
TGAATCCTTCTGTCAATTGTCACTC 473 575 269 Antisense
ATTCATCATTCTTGTTGTTCATTGA 696 11 301 Antisense
CCCACCACCAGCAAATACAGTTCTC 311 257 373 Antisense
Sequence Source Affymetrix Proprietary Database