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[174624_at]

Description CEK041A5R_rc /REP_DB=TREMBL Accession /GB=D34103 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41a5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 18      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 0      6grp_total > 05      4grp_total > 22      6grp_cgc > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 464
1 2 3 4 range
A 1299.6
(-0)
P 		1365.9
(3)
P 		1274.2
(-1.2)
P 		1235.2
(-3)
P 		131
B 1520.1
(1.4)
P 		1260.1
(-2)
P 		1182.3
(-3)
P 		1646.6
(3)
P 		464
C 1356.3
(3)
P 		1275.1
(-2.1)
P 		1338.6
(1.9)
P 		1261
(-3)
P 		95
avr 1392
(3) 		1300.4
(-1.3) 		1265
(-3) 		1380.9
(2.5) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1058
1 2 3 4 5 6 range
A 1877.4
(3)
P 		1756.7
(1.9)
P 		1321.3
(-2)
P 		1210.6
(-3)
P 		1722.8
(1.6)
P 		1440.5
(-0.9)
P 		667
B 1904
(3)
P 		1795.5
(1.9)
P 		1547.5
(-0.5)
P 		1555.8
(-0.4)
P 		1367.5
(-2.3)
P 		1296.1
(-3)
P 		608
C 2134.7
(3)
P 		1566.1
(-0.2)
P 		1475.3
(-0.7)
P 		1076.4
(-3)
P 		1643.9
(0.2)
P 		1265.1
(-1.9)
P 		1058
avr 1972
(3) 		1706.1
(0.7) 		1448
(-1.5) 		1280.9
(-3) 		1578.1
(-0.4) 		1333.9
(-2.5) 		691
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 304
1 2 3 4 range
A 473.2
(3)
P 		296.5
(-0.6)
P 		333.1
(0.1)
P 		180.8
(-3)
P 		292
B 484.7
(3)
P 		311.2
(-1.9)
P 		274
(-3)
P 		350.9
(-0.8)
P 		211
C 449
(2.6)
P 		366.6
(0.6)
P 		466.5
(3)
P 		212.6
(-3)
P 		254
avr 469
(3) 		324.8
(-0.9) 		357.9
(-0) 		248.1
(-3) 		221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 575
1 2 3 4 5 6 range
A 346.1
(-2)
P 		624.8
(3)
P 		287.7
(-3)
P 		307
(-2.7)
P 		519.9
(1.1)
P 		350.7
(-1.9)
P 		337
B 662.5
(3)
P 		597.7
(1.6)
P 		375.8
(-3)
P 		412.1
(-2.2)
P 		555.6
(0.8)
P 		483.3
(-0.8)
P 		287
C 791.2
(3)
P 		477.9
(-0.3)
P 		375.5
(-1.3)
P 		216.5
(-3)
P 		532
(0.3)
P 		423.5
(-0.8)
P 		575
avr 599.9
(3) 		566.8
(2.3) 		346.3
(-2.3) 		311.9
(-3) 		535.8
(1.7) 		419.2
(-0.8) 		288
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174624_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21646
Sequence Type Exemplar sequence
Representative Public ID CEK041A5R NCBI
Target Description CEK041A5R_rc /REP_DB=TREMBL Accession /GB=D34103 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk41a5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067740 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-4) (egl-4) mRNA, complete cds. 11/11 None
F55A8.2a.2 ENSEMBL cdna:known chromosome:CEL140:IV:1847964:1880574:1 gene:F55A8.2 11/11 A 
F55A8.2a.1 ENSEMBL cdna:known chromosome:CEL140:IV:1847966:1880586:1 gene:F55A8.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F55A8.2a.3 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176991 6 1 NM_001028052 192802_s_at (11)
NM_001028053 192802_s_at (11)
NM_067740 174624_at (11), 192802_s_at (11)
NM_067741 192802_s_at (11)
NM_171279 192802_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1880176-1880571(+) 100.0 100.0
Public Domain and Genome References
Ensembl F55A8.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD36954 Egg laying defective protein 4, isoform a [Caenorhabditis elegans] ref|NP_500141.1| EGg Laying defective family member (egl-4) [Caenorhabditis elegans] 0.0
blast AAD36953 Egg laying defective protein 4, isoform b [Caenorhabditis elegans] ref|NP_500142.1| EGg Laying defective family member (egl-4) [Caenorhabditis elegans] 0.0
blast AAU05567 Egg laying defective protein 4, isoform e [Caenorhabditis elegans] ref|NP_001023223.1| EGg Laying defective family member (egl-4) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 1.1.18 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DG2 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-76
Pfam IPR000595 EMBL-EBI Cyclic nucleotide-binding domain 2.1E-28
Pfam IPR000595 EMBL-EBI Cyclic nucleotide-binding domain 3.5E-27
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.6E-4
Sequence
Target Sequence 
>C. ELEGANS:174624_AT
ctttttccaccatgttcactgagattctatgtgatcttttccggtttctctatgattttt
tgaaactctaccgccactattttagtattctcgaattttcatatattcaccccctcctcc
tcttttttctcagttcctcattttcagtctaacaccattttttagtttcccaacaaaaaa
gtccgagagaaaactcggcaaactacaccaccaccaggtaatattattattttctaatcc
ccccctaatactgatatatctctattttcatttctatctaaaatccaatcattcgaaatt
gaactatacatacttttttttctttgctctctctcttatgtctcgattctatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTTTTCCACCATGTTCACTGAGAT 314 223 22 Antisense
ATTCTATGTGATCTTTTCCGGTTTC 279 11 45 Antisense
TCTTTTCCGGTTTCTCTATGATTTT 526 615 56 Antisense
TGATTTTTTGAAACTCTACCGCCAC 279 561 74 Antisense
AACTCTACCGCCACTATTTTAGTAT 382 137 85 Antisense
CGAATTTTCATATATTCACCCCCTC 354 253 113 Antisense
TCTCAGTTCCTCATTTTCAGTCTAA 426 613 149 Antisense
CATTTTCAGTCTAACACCATTTTTT 426 215 160 Antisense
AATCCCCCCCTAATACTGATATATC 82 167 257 Antisense
TATCTCTATTTTCATTTCTATCTAA 579 659 278 Antisense
TCTCTCTTATGTCTCGATTCTATAT 689 611 351 Antisense
Sequence Source Affymetrix Proprietary Database