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[174622_at]

Description CEK072B5R_rc /REP_DB=TREMBL Accession /GB=D66003 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk72b5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 19      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 30      6grp_total > 29      4grp_total > 15      6grp_cgc > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 828
1 2 3 4 range
A 1182.1
(3)
P 		506.6
(-3)
P 		670.4
(-1.5)
P 		766.2
(-0.7)
P 		676
B 784
(3)
P 		534.6
(-0.6)
P 		363.1
(-3)
P 		780.7
(3)
P 		421
C 812.8
(-0.4)
P 		527.4
(-3)
P 		796.1
(-0.6)
P 		1191.1
(3)
P 		664
avr 926.3
(3) 		522.9
(-3) 		609.9
(-1.7) 		912.7
(2.8) 		403

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 806
1 2 3 4 5 6 range
A 692.6
(0.7)
P 		880
(3)
P 		400.2
(-3)
P 		628.5
(-0.1)
P 		787.3
(1.8)
P 		664.3
(0.3)
P 		480
B 1190.2
(3)
P 		988.3
(1.5)
P 		383.8
(-3)
P 		576
(-1.6)
P 		756
(-0.2)
P 		693.2
(-0.7)
P 		806
C 1114.9
(3)
P 		756.5
(-0.3)
P 		611.6
(-1.6)
P 		457
(-3)
P 		901.2
(1.1)
P 		598.6
(-1.7)
P 		658
avr 999.2
(3) 		874.9
(1.6) 		465.2
(-3) 		553.8
(-2) 		814.8
(0.9) 		652
(-0.9) 		534
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 346
1 2 3 4 range
A 430.4
(3)
P 		110
(-3)
P 		175.2
(-1.8)
P 		112.2
(-3)
P 		320
B 250
(3)
P 		132
(-1.3)
P 		84.1
(-3)
P 		166.4
(-0)
P 		166
C 269
(2.6)
P 		151.6
(-3)
P 		277.5
(3)
P 		200.8
(-0.7)
P 		126
avr 316.5
(3) 		131.2
(-3) 		178.9
(-1.5) 		159.8
(-2.1) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 327
1 2 3 4 5 6 range
A 127.7
(-1.9)
P 		313
(3)
P 		87.2
(-3)
P 		159.4
(-1.1)
P 		237.6
(1)
P 		161.7
(-1)
P 		226
B 414.1
(3)
P 		329
(1.4)
P 		93.2
(-3)
P 		152.6
(-1.9)
P 		307.1
(1)
P 		258.5
(0.1)
P 		321
C 413.3
(3)
P 		230.8
(-0.4)
P 		155.7
(-1.8)
P 		91.9
(-3)
P 		291.6
(0.7)
P 		200.4
(-1)
P 		321
avr 318.4
(3) 		290.9
(2.2) 		112
(-3) 		134.6
(-2.3) 		278.8
(1.8) 		206.9
(-0.2) 		206
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174622_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21637
Sequence Type Exemplar sequence
Representative Public ID CEK072B5R NCBI
Target Description CEK072B5R_rc /REP_DB=TREMBL Accession /GB=D66003 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk72b5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description Only library file information is available for this probe set. See probe design information above.
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
B0545.1b.2 ENSEMBL cdna:known chromosome:CEL140:IV:92803:110707:1 gene:B0545.1 11/11 None
B0545.1b.1 ENSEMBL cdna:known chromosome:CEL140:IV:103444:110707:1 gene:B0545.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
B0545.1a 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:110433-110697(+) 89.02 100.0
Public Domain and Genome References
Ensembl B0545.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26917 Tpa (tetradecanoyl phorbol acetate) resistant protein 1, isoform b [Caenorhabditis elegans] ref|NP_499861.1| TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) [Caenorhabditis elegans] dbj|BAA08471.1| TPA-1B [Caenorhabditis elegans] 0.0
blast AAC26916 Tpa (tetradecanoyl phorbol acetate) resistant protein 1, isoform a [Caenorhabditis elegans] ref|NP_499860.1| TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) [Caenorhabditis elegans] dbj|BAA08470.1| TPA-1A [Caenorhabditis elegans] sp|P34722|KPC1_CAEEL Protein kinase C-like 1 (PKC) (Tetradecanoyl phorbol acetate resistant protein 1) 0.0
blast T33399 protein kinase C homolog tpa-1, splice form 1 - Caenorhabditis elegans 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-51
hanks 1.2.11 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; CeTPA1 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.8E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-71
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-32
Sequence
Target Sequence 
>C. ELEGANS:174622_AT
gatgccacgtgtataaaaattcactgatcagtaaagactgcttctccatatagatttttt
ttgataattaaattttaaataaccccgccccctccctggccacgcccattaatttgtatt
tcatatcacttgacatctctccccgatttttgtctaattttcaactttttttttacaata
ttgaatttcccacgcttttttgtcttacttccatattttctttgtcagagcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCCACGTGTATAAAAATTCACT 446 413 13 Antisense
ACTGATCAGTAAAGACTGCTTCTCC 81 99 35 Antisense
AAGACTGCTTCTCCATATAGATTTT 684 153 46 Antisense
GCTTCTCCATATAGATTTTTTTTGA 694 305 52 Antisense
TGGCCACGCCCATTAATTTGTATTT 603 543 109 Antisense
CACGCCCATTAATTTGTATTTCATA 376 199 113 Antisense
TTTCATATCACTTGACATCTCTCCC 155 673 131 Antisense
ATATTGAATTTCCCACGCTTTTTTG 200 19 190 Antisense
GCTTTTTTGTCTTACTTCCATATTT 332 305 206 Antisense
GTCTTACTTCCATATTTTCTTTGTC 611 467 214 Antisense
TCCATATTTTCTTTGTCAGAGCATT 513 591 222 Antisense
Sequence Source Affymetrix Proprietary Database