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[174621_at]

Description CEK004AXR_rc /REP_DB=TREMBL Accession /GB=D27798 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4a10 : 3prime end, single read.
Cluster 6grp_total > 13      4grp_total > 07      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 40.6
(0.1)
A 		45.2
(2.3)
P 		34.1
(-3)
A 		46.6
(3)
A 		13
B 48.1
(2.5)
A 		22.8
(-3)
A 		50.5
(3)
A 		39.1
(0.5)
A 		28
C 35.4
(-0.1)
A 		23
(-2.4)
A 		19.9
(-3)
A 		51.9
(3)
A 		32
avr 41.4
(1.3) 		30.3
(-3) 		34.8
(-1.3) 		45.9
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 63.2
(3)
A 		37.5
(-0.3)
A 		40.8
(0.1)
A 		32
(-1)
A 		16.4
(-3)
A 		20.9
(-2.4)
A 		47
B 41.6
(1)
A 		43.9
(1.4)
A 		38.9
(0.6)
A 		54.1
(3)
M 		43.4
(1.3)
P 		16.3
(-3)
A 		38
C 78.6
(3)
P 		73.6
(2.5)
A 		41
(-1.1)
A 		61.2
(1.1)
A 		36.3
(-1.6)
A 		23.3
(-3)
A 		55
avr 61.1
(3) 		51.7
(1.6) 		40.2
(-0.1) 		49.1
(1.2) 		32
(-1.3) 		20.2
(-3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 14.8
(3)
A 		9.8
(-0.7)
P 		8.9
(-1.4)
A 		6.8
(-3)
A 		8
B 15.3
(3)
A 		5.6
(-3)
A 		11.7
(0.8)
A 		8.3
(-1.3)
A 		10
C 11.7
(3)
A 		6.6
(-3)
A 		6.9
(-2.6)
A 		8.8
(-0.4)
A 		5
avr 13.9
(3) 		7.3
(-3) 		9.2
(-1.3) 		8
(-2.4) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 11.6
(1.8)
A 		13.3
(3)
A 		8.9
(-0.1)
A 		8.1
(-0.7)
A 		4.9
(-3)
A 		5.1
(-2.9)
A 		8
B 14.5
(1.4)
A 		14.6
(1.4)
A 		9.5
(-1.2)
A 		14.3
(1.3)
M 		17.6
(3)
P 		6.1
(-3)
A 		12
C 29.1
(3)
P 		22.5
(1.1)
A 		10.4
(-2.3)
A 		12.3
(-1.7)
A 		11.7
(-1.9)
A 		7.8
(-3)
A 		21
avr 18.4
(3) 		16.8
(2.2) 		9.6
(-1.4) 		11.6
(-0.4) 		11.4
(-0.5) 		6.3
(-3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174621_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22101
Sequence Type Exemplar sequence
Representative Public ID CEK004AXR NCBI
Target Description CEK004AXR_rc /REP_DB=TREMBL Accession /GB=D27798 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4a10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029788(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029788 NCBI Caenorhabditis elegans Y34B4A.4b (Y34B4A.4) mRNA, complete cds. 11/11 None
Y34B4A.4b ENSEMBL cdna:known chromosome:CEL140:X:5264295:5271753:1 gene:Y34B4A.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180798 2 1 NM_001029788 174621_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5271358-5271744(+) 93.26 100.0
Public Domain and Genome References
Ensembl Y34B4A.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59639 Hypothetical protein Y34B4A.4a [Caenorhabditis elegans] ref|NP_001024958.1| Y34B4A.4a [Caenorhabditis elegans] 0.0
blast AAO91688 Hypothetical protein Y34B4A.4b [Caenorhabditis elegans] ref|NP_001024959.1| Y34B4A.4b [Caenorhabditis elegans] 0.0
blast CAE68723 Hypothetical protein CBG14649 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.4E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 7.6E-12
Sequence
Target Sequence 
>C. ELEGANS:174621_AT
gaggtcgttattgtgactaggacacactaggcgactccacttattcatcattcaataacc
cactagataatctcaatctaaatcattcaggaattatgacataaagaagaacgacatcgt
agtgcagtattttaatggctttaaagactgtatcgatgaggagcgcacttggcaactttc
tcatcaaattaagccgtcaaaccgaaagtccaaaacacaggcataatatacaatccacaa
ttcacagcccctgtcattatttctcagatttcttttctcactttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGTCGTTATTGTGACTAGGACAC 128 399 37 Antisense
ACTAGGACACACTAGGCGACTCCAC 422 99 52 Antisense
ACACTAGGCGACTCCACTTATTCAT 451 97 60 Antisense
CGACTCCACTTATTCATCATTCAAT 84 253 68 Antisense
ATTCATCATTCAATAACCCACTAGA 677 11 79 Antisense
TTCAATAACCCACTAGATAATCTCA 503 683 87 Antisense
GATAATCTCAATCTAAATCATTCAG 225 423 102 Antisense
GCATAATATACAATCCACAATTCAC 174 307 257 Antisense
ACAATTCACAGCCCCTGTCATTATT 272 115 273 Antisense
CAGCCCCTGTCATTATTTCTCAGAT 541 203 281 Antisense
TTCTCAGATTTCTTTTCTCACTTTC 123 693 297 Antisense
Sequence Source Affymetrix Proprietary Database