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[174619_at]

Description CEK004AYR_rc /REP_DB=TREMBL Accession /GB=D27800 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4a11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 06      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 788
1 2 3 4 range
A 857.9
(3)
P 		340
(-1.2)
P 		164.9
(-2.6)
P 		119
(-3)
P 		739
B 894.1
(3)
P 		194.9
(-2.9)
P 		210
(-2.8)
P 		184.3
(-3)
P 		710
C 742.4
(3)
P 		288.2
(-1.3)
P 		224.2
(-1.9)
P 		105.7
(-3)
P 		637
avr 831.5
(3) 		274.4
(-1.8) 		199.7
(-2.5) 		136.3
(-3) 		695

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 764
1 2 3 4 5 6 range
A 833.1
(3)
P 		356.1
(-1)
P 		260.4
(-1.8)
P 		162.9
(-2.6)
P 		193.1
(-2.4)
P 		120.3
(-3)
P 		713
B 861.6
(3)
P 		548.1
(0.5)
P 		452.5
(-0.2)
P 		236
(-1.9)
P 		188.2
(-2.3)
P 		97.3
(-3)
P 		764
C 733.3
(3)
P 		523.1
(0.9)
P 		240.5
(-2)
P 		172.3
(-2.7)
P 		144.1
(-2.9)
P 		137.7
(-3)
P 		596
avr 809.3
(3) 		475.8
(0.1) 		317.8
(-1.3) 		190.4
(-2.4) 		175.1
(-2.5) 		118.4
(-3) 		691
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 312.4
(3)
P 		73.8
(-1.9)
P 		43.1
(-2.5)
P 		17.4
(-3)
P 		295
B 285.1
(3)
P 		48.1
(-2.8)
P 		48.7
(-2.8)
P 		39.3
(-3)
P 		246
C 245.7
(3)
P 		82.9
(-1.3)
P 		78.1
(-1.4)
P 		17.8
(-3)
P 		228
avr 281.1
(3) 		68.3
(-2) 		56.6
(-2.3) 		24.8
(-3) 		256

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 271
1 2 3 4 5 6 range
A 153.6
(3)
P 		126.7
(1.7)
P 		56.7
(-1.7)
P 		41.3
(-2.4)
P 		58.3
(-1.6)
P 		29.3
(-3)
P 		124
B 299.8
(3)
P 		182.4
(0.3)
P 		109.9
(-1.3)
P 		62.5
(-2.4)
P 		76.5
(-2.1)
P 		36.3
(-3)
P 		264
C 271.8
(3)
P 		159.6
(0.2)
P 		61.2
(-2.3)
P 		34.6
(-3)
P 		46.6
(-2.7)
P 		46.1
(-2.7)
P 		237
avr 241.7
(3) 		156.2
(0.5) 		75.9
(-1.9) 		46.1
(-2.7) 		60.5
(-2.3) 		37.2
(-3) 		205
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174619_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21106
Sequence Type Exemplar sequence
Representative Public ID CEK004AYR NCBI
Target Description CEK004AYR_rc /REP_DB=TREMBL Accession /GB=D27800 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4a11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027419(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027419 NCBI Caenorhabditis elegans F45G2.2a (F45G2.2) mRNA, complete cds. 11/11 None
F45G2.2a ENSEMBL cdna:known chromosome:CEL140:III:13409437:13423042:1 gene:F45G2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027420 5/11 Cross Hyb Matching Probes None
GENEFINDER00000011678 5/11 Cross Hyb Matching Probes None
SNAP00000011667 5/11 Cross Hyb Matching Probes None
F45G2.2b.2 5/11 Cross Hyb Matching Probes None
F45G2.2b.1 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176764 2 1 NM_001027419 174619_at (11), 193933_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13422748-13423017(+) 87.73 100.0
Public Domain and Genome References
Ensembl F45G2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07611 Hypothetical protein F45G2.2a [Caenorhabditis elegans] ref|NP_001022590.1| F45G2.2a [Caenorhabditis elegans] 0.0
blast CAE73701 Hypothetical protein CBG21212 [Caenorhabditis briggsae] 0.0
blast AAG24132 Hypothetical protein F58G4.1 [Caenorhabditis elegans] ref|NP_505094.1| F58G4.1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 4.5E-14
Pfam IPR002928 EMBL-EBI Myosin tail 5.4E-14
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174619_AT
tcttcgtattttggatcaccgccgtgccctcagcaagcaaccacttccgagagtttacgt
cgaggatccggatagtgattcggactaaagaaaattgatatattttaccaaaccttctct
tttagttttttttcttgtactttatcttctgaccctctttactctggcgccaactactca
aataatttcactttcagttttactct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCGTATTTTGGATCACCGCCGT 494 617 29 Antisense
GCAAGCAACCACTTCCGAGAGTTTA 213 319 61 Antisense
GCAACCACTTCCGAGAGTTTACGTC 57 321 65 Antisense
GAGAGTTTACGTCGAGGATCCGGAT 442 395 77 Antisense
GGATCCGGATAGTGATTCGGACTAA 165 513 92 Antisense
GATATATTTTACCAAACCTTCTCTT 369 427 125 Antisense
TTTTTTTCTTGTACTTTATCTTCTG 410 675 155 Antisense
TTCTTGTACTTTATCTTCTGACCCT 664 689 160 Antisense
TTTACTCTGGCGCCAACTACTCAAA 226 673 186 Antisense
GCGCCAACTACTCAAATAATTTCAC 28 287 195 Antisense
ATAATTTCACTTTCAGTTTTACTCT 582 25 210 Antisense
Sequence Source Affymetrix Proprietary Database