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[174616_at]

Description CEK008FZR_rc /REP_DB=TREMBL Accession /GB=D34560 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk8f12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 59      4grp_high_8_8 > 26      6grp_highest_nonorm > 91      6grp_total > 39      4grp_total > 38      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 285.8
(3)
P 		241.2
(0.7)
P 		169.4
(-2.9)
P 		168.2
(-3)
P 		118
B 252.1
(3)
P 		206.8
(1)
P 		113.8
(-3)
P 		210.7
(1.2)
P 		138
C 336.2
(3)
P 		174.6
(-3)
P 		196.9
(-2.2)
P 		222.8
(-1.2)
P 		162
avr 291.4
(3) 		207.5
(-0.8) 		160
(-3) 		200.6
(-1.1) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 276.8
(3)
P 		223
(0.5)
P 		150.2
(-3)
P 		163.6
(-2.4)
P 		263.7
(2.4)
P 		230.1
(0.8)
P 		127
B 306.3
(3)
P 		195.5
(-0)
P 		86.4
(-3)
P 		152.6
(-1.2)
P 		218
(0.6)
P 		282.2
(2.3)
P 		220
C 298.7
(1.7)
P 		220.8
(-1)
P 		179.6
(-2.5)
P 		165
(-3)
P 		334
(3)
P 		244.4
(-0.2)
P 		169
avr 293.9
(3) 		213.1
(-0.1) 		138.7
(-3) 		160.4
(-2.2) 		271.9
(2.1) 		252.2
(1.4) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 104.1
(3)
P 		52.4
(-0.9)
P 		44.3
(-1.5)
P 		24.6
(-3)
P 		80
B 80.4
(3)
P 		51.1
(-0.3)
P 		26.4
(-3)
P 		44.9
(-0.9)
P 		54
C 111.3
(3)
P 		50.2
(-2)
P 		68.6
(-0.5)
P 		37.6
(-3)
P 		74
avr 98.6
(3) 		51.2
(-1.5) 		46.4
(-2) 		35.7
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 51
(-0.7)
P 		79.3
(3)
P 		32.7
(-3)
P 		41.5
(-1.9)
P 		79.6
(3)
P 		56
(-0)
P 		47
B 106.6
(3)
P 		65.1
(0.1)
P 		21
(-3)
P 		40.4
(-1.6)
P 		88.6
(1.7)
P 		105.2
(2.9)
P 		86
C 110.7
(3)
P 		67.4
(-0.4)
P 		45.7
(-2)
P 		33.2
(-3)
P 		108.1
(2.8)
P 		81.8
(0.8)
P 		78
avr 89.4
(2.7) 		70.6
(0.8) 		33.1
(-3) 		38.4
(-2.5) 		92.1
(3) 		81
(1.9) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174616_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21767
Sequence Type Exemplar sequence
Representative Public ID CEK008FZR NCBI
Target Description CEK008FZR_rc /REP_DB=TREMBL Accession /GB=D34560 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk8f12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description Only library file information is available for this probe set. See probe design information above.
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T19A5.2a ENSEMBL cdna:known chromosome:CEL140:V:8950398:8958880:1 gene:T19A5.2 11/11 None
T19A5.2c ENSEMBL cdna:known chromosome:CEL140:V:8951623:8958880:1 gene:T19A5.2 11/11 None
T19A5.2d ENSEMBL cdna:known chromosome:CEL140:V:8952138:8958880:1 gene:T19A5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8958501-8958856(+) 100.0 100.0
Public Domain and Genome References
Ensembl T19A5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69038 Germinal center kinase family protein 1, isoform a [Caenorhabditis elegans] ref|NP_505309.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAT81184 Germinal center kinase family protein 1, isoform d [Caenorhabditis elegans] ref|NP_001024141.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAK77641 Germinal center kinase family protein 1, isoform b [Caenorhabditis elegans] ref|NP_505310.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAK77642 Germinal center kinase family protein 1, isoform c [Caenorhabditis elegans] ref|NP_505311.2| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-63
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 9.0E-69
hanks 2.1.5 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DmCamKII 3.0E-76
hanks 1.9.6 AGC group; AGC Other; SPK1 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Pfam IPR000719 EMBL-EBI Protein kinase 7.8E-87
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Sequence
Target Sequence 
>C. ELEGANS:174616_AT
ttaaccaaacttttcgatgatcaccggtctcattctccccatttaggtcatttttctcat
gtttattttatttgtgtgtgtgtagttttaataactatttctctttttttcatttataat
atctctcattgctctacatatgttcatatgtagagaaaagagagattactatttccgcct
ttatcacttttttttctcatttgtttaatttaaacgcggttattcctcttcacttttccc
tcccaccctttttttgctccaaatcaaaaagtcttccattgactggatttattttcatta
gttcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTAACCAAACTTTTCGATGATCACC 84 677 14 Antisense
TCATTCTCCCCATTTAGGTCATTTT 95 619 43 Antisense
TCCCCATTTAGGTCATTTTTCTCAT 239 597 49 Antisense
TAGGTCATTTTTCTCATGTTTATTT 351 655 57 Antisense
GTTTATTTTATTTGTGTGTGTGTAG 389 447 74 Antisense
TTATAATATCTCTCATTGCTCTACA 533 679 127 Antisense
CTCTCATTGCTCTACATATGTTCAT 451 231 136 Antisense
AGAGATTACTATTTCCGCCTTTATC 240 69 174 Antisense
TGCTCCAAATCAAAAAGTCTTCCAT 114 583 268 Antisense
CAAAAAGTCTTCCATTGACTGGATT 369 187 278 Antisense
CTGGATTTATTTTCATTAGTTCATT 102 241 296 Antisense
Sequence Source Affymetrix Proprietary Database