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[174612_at]

Description CEK049H4R_rc /REP_DB=TREMBL Accession /GB=D64350 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk49h4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 20      6grp_total > 27      4grp_total > 38      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 578
1 2 3 4 range
A 1233.3
(3)
P 		1000
(0.2)
P 		738.3
(-3)
P 		983.9
(-0)
P 		495
B 1095.4
(3)
P 		830.2
(-1.4)
P 		737.2
(-3)
P 		992.1
(1.3)
P 		358
C 1315.1
(3)
P 		848.3
(-3)
P 		936.3
(-1.9)
P 		967.7
(-1.5)
P 		467
avr 1214.6
(3) 		892.8
(-1.7) 		803.9
(-3) 		981.2
(-0.4) 		411

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1140
1 2 3 4 5 6 range
A 1724
(3)
P 		1074.7
(-0.6)
P 		640
(-3)
P 		638.8
(-3)
P 		965.5
(-1.2)
P 		700.3
(-2.7)
P 		1085
B 1627.7
(3)
P 		1168.2
(-0.1)
P 		738.8
(-3)
P 		861.8
(-2.2)
P 		808.2
(-2.5)
P 		1160.6
(-0.2)
P 		889
C 1220.1
(3)
P 		1159.2
(2.4)
P 		742.8
(-1.5)
P 		583.7
(-3)
P 		756.2
(-1.4)
P 		791.9
(-1)
P 		636
avr 1523.9
(3) 		1134
(0.2) 		707.2
(-2.9) 		694.8
(-3) 		843.3
(-1.9) 		884.3
(-1.6) 		829
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 305
1 2 3 4 range
A 449
(3)
P 		217.1
(-1.6)
P 		193
(-2)
P 		144
(-3)
P 		305
B 349.3
(3)
P 		205
(-1.9)
P 		170.8
(-3)
P 		211.4
(-1.6)
P 		179
C 435.3
(3)
P 		243.9
(-1.2)
P 		326.3
(0.6)
P 		163.2
(-3)
P 		272
avr 411.2
(3) 		222
(-1.8) 		230
(-1.6) 		172.9
(-3) 		238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 449
1 2 3 4 5 6 range
A 317.8
(1.4)
P 		382.2
(3)
P 		139.4
(-3)
P 		162
(-2.4)
P 		291.3
(0.8)
P 		170.5
(-2.2)
P 		243
B 566.4
(3)
P 		388.8
(0.2)
P 		179.4
(-3)
P 		228.3
(-2.2)
P 		328.3
(-0.7)
P 		432.8
(0.9)
P 		387
C 452.2
(3)
P 		353.7
(1.2)
P 		189.1
(-1.7)
P 		117.4
(-3)
P 		244.7
(-0.7)
P 		265.1
(-0.4)
P 		335
avr 445.5
(3) 		374.9
(1.5) 		169.3
(-3) 		169.2
(-3) 		288.1
(-0.4) 		289.5
(-0.4) 		276
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174612_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21359
Sequence Type Exemplar sequence
Representative Public ID CEK049H4R NCBI
Target Description CEK049H4R_rc /REP_DB=TREMBL Accession /GB=D64350 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk49h4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069157(11), NM_001027922(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069157 NCBI Caenorhabditis elegans C53B4.4b (C53B4.4) mRNA, complete cds. 11/11 None
NM_001027922 NCBI Caenorhabditis elegans C53B4.4d (C53B4.4) mRNA, complete cds. 11/11 None
C53B4.4b ENSEMBL cdna:known chromosome:CEL140:IV:8973678:8984686:1 gene:C53B4.4 11/11 A 
C53B4.4d ENSEMBL cdna:known chromosome:CEL140:IV:8975617:8984686:1 gene:C53B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177716 4 2 NM_001027922 174612_at (11)
NM_069157 174612_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8984199-8984682(+) 100.0 100.0
Public Domain and Genome References
Ensembl C53B4.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92456 Hypothetical protein C53B4.4b [Caenorhabditis elegans] ref|NP_501558.1| C53B4.4b [Caenorhabditis elegans] 0.0
blast CAA92457 Hypothetical protein C53B4.4a [Caenorhabditis elegans] ref|NP_501559.1| C53B4.4a [Caenorhabditis elegans] 0.0
blast CAE74079 Hypothetical protein CBG21734 [Caenorhabditis briggsae] 0.0
blast CAH60786 Hypothetical protein C53B4.4d [Caenorhabditis elegans] ref|NP_001023093.1| C53B4.4d [Caenorhabditis elegans] 0.0
blast CAB54213 Hypothetical protein C53B4.4c [Caenorhabditis elegans] ref|NP_501560.2| C53B4.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 2.0E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 2.0E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501558.1 2 110-132,145-164
NP_001023093.1 1 2-24
C53B4.4b 2 110-132,145-164
C53B4.4d 1 2-24
Sequence
Target Sequence 
>C. ELEGANS:174612_AT
actttcttttctccaaatctgtgatatataaaatctcgaaccagtccctccatcccgagc
cagtgcaccaaaatttgtcgaaagtattttgtcttgcaactgtgatttttactattccta
atcttattcgtgtattcatcaaatttaaaattccatacgcaccaaatcaagttttgatga
ctatacatacttcatcattctctgaatccccatcgaaaaactgcataatttttatttatt
gactcgcgaaaaattgaaaatgtcattccctattgcttcccacgaattgtccctaaaaca
ctgtacgtttcacataaatctaatttatcttttaagtccttctttttcaaagttcttggt
tcaaaaaatatctcgtcatttcggctagaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTCTTTTCTCCAAATCTGTGAT 220 103 36 Antisense
ATATAAAATCTCGAACCAGTCCCTC 543 13 61 Antisense
TCCCGAGCCAGTGCACCAAAATTTG 204 597 88 Antisense
GCAACTGTGATTTTTACTATTCCTA 51 321 131 Antisense
ACTATTCCTAATCTTATTCGTGTAT 624 101 146 Antisense
AATTCCATACGCACCAAATCAAGTT 452 177 184 Antisense
GACTATACATACTTCATCATTCTCT 320 373 214 Antisense
TCATTCTCTGAATCCCCATCGAAAA 622 619 230 Antisense
GAAAATGTCATTCCCTATTGCTTCC 438 353 291 Antisense
GAATTGTCCCTAAAACACTGTACGT 241 323 319 Antisense
AATATCTCGTCATTTCGGCTAGACA 708 173 402 Antisense
Sequence Source Affymetrix Proprietary Database