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[174591_at] dynactin

Description CEK011C8R_rc /REP_DB=TREMBL Accession /GB=D32344 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11c8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 50      6grp_total > 18      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1056
1 2 3 4 range
A 1089.4
(3)
P 		289
(-3)
P 		373.8
(-2.4)
P 		530.4
(-1.2)
P 		800
B 844.7
(3)
P 		320.6
(-3)
P 		365.5
(-2.5)
P 		563.9
(-0.2)
P 		524
C 1344.6
(3)
P 		463.1
(-3)
P 		640.9
(-1.8)
P 		541
(-2.5)
P 		882
avr 1092.9
(3) 		357.6
(-3) 		460.1
(-2.2) 		545.1
(-1.5) 		735

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 794
1 2 3 4 5 6 range
A 515.1
(1.1)
P 		558.2
(1.9)
P 		395
(-1.3)
P 		307.2
(-3)
P 		612.1
(3)
P 		475.7
(0.3)
P 		305
B 873.6
(3)
P 		589.6
(-0.3)
P 		536.3
(-1)
P 		688.2
(0.8)
P 		364.2
(-3)
P 		582.4
(-0.4)
P 		509
C 1101.3
(3)
P 		461.3
(-2.6)
P 		472.7
(-2.5)
P 		442.3
(-2.8)
P 		413.8
(-3)
P 		483
(-2.4)
P 		688
avr 830
(3) 		536.4
(-1.8) 		468
(-2.9) 		479.2
(-2.7) 		463.4
(-3) 		513.7
(-2.2) 		367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 396.7
(3)
P 		62.7
(-3)
P 		97.7
(-2.4)
P 		77.6
(-2.7)
P 		334
B 269.4
(3)
P 		79.2
(-3)
P 		84.7
(-2.8)
P 		120.2
(-1.7)
P 		190
C 445.1
(3)
P 		133.1
(-2.3)
P 		223.4
(-0.8)
P 		91.2
(-3)
P 		354
avr 370.4
(3) 		91.7
(-3) 		135.3
(-2.1) 		96.3
(-2.9) 		279

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 95
(-2.1)
P 		198.5
(3)
P 		86
(-2.6)
P 		77.9
(-3)
P 		184.7
(2.3)
P 		115.8
(-1.1)
P 		121
B 304
(3)
P 		196.3
(-0.7)
P 		130.2
(-3)
P 		182.3
(-1.2)
P 		148
(-2.4)
P 		217.2
(0)
P 		174
C 408.2
(3)
P 		140.8
(-2)
P 		120.3
(-2.4)
P 		88.9
(-3)
P 		133.9
(-2.2)
P 		161.7
(-1.6)
P 		319
avr 269.1
(3) 		178.5
(-0.5) 		112.2
(-3) 		116.4
(-2.8) 		155.5
(-1.3) 		164.9
(-1) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174591_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21382
Sequence Type Exemplar sequence
Representative Public ID CEK011C8R NCBI
Target Description CEK011C8R_rc /REP_DB=TREMBL Accession /GB=D32344 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11c8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069632(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069632 NCBI Caenorhabditis elegans ZK593.5 (dynactin) mRNA, complete cds. 11/11 None
ZK593.5 ENSEMBL cdna:known chromosome:CEL140:IV:10926702:10933092:-1 gene:ZK593.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000042105 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10926717-10927313(-) 100.0 100.0
Public Domain and Genome References
Gene Title dynactin
Gene Symbol dnc-1
Ensembl ZK593.5
Entrez Gene 177987 Entrez gene
SwissProt Q23542 EMBL-EBI
WormBase CE30718 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17183.1.S1_AT similar to dynactin 1 isoform 1 cfa
CANINE_2:CFA.18089.1.S1_S_AT similar to dynactin 1 isoform 1 cfa
CANINE_2:CFA.21128.1.S1_S_AT similar to dynactin 1 isoform 1 cfa
CANINE_2:CFAAFFX.13659.1.S1_S_AT similar to dynactin 1 isoform 1 cfa
DROSOPHILA_2:1626483_AT Glued dm
DROSGENOME1:143177_AT Glued dm
HG-U133A:201082_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133_PLUS_2:201082_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133A_2:201082_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-FOCUS:201082_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133_PLUS_2:204296_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133A:204296_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-FOCUS:204296_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133A_2:204296_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133_PLUS_2:211780_X_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133A:211780_X_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U133A_2:211780_X_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HUGENEFL:X98801_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HUGENEFL:U73799_AT dynactin 1 (p150, glued homolog, Drosophila) hs
U133_X3P:G13544059_3P_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
U133_X3P:G13259509_3P_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
U133_X3P:G10864004_3P_AT dynactin 1 (p150, glued homolog, Drosophila) hs
U133_X3P:211780_3P_X_AT dynactin 1 (p150, glued homolog, Drosophila) hs
U133_X3P:201082_3P_S_AT dynactin 1 (p150, glued homolog, Drosophila) hs
HG-U95AV2:36158_AT dynactin 1 (p150, glued homolog, Drosophila) hs
MU11KSUBA:U60312_S_AT dynactin 1 mm
MU11KSUBB:MSA.11860.0_AT dynactin 1 mm
MU11KSUBB:MSA.5854.0_S_AT dynactin 1 mm
MU11KSUBB:MSA.19580.0_S_AT dynactin 1 mm
MU11KSUBB:MSA.12975.0_S_AT dynactin 1 mm
MG-U74AV2:101367_AT dynactin 1 mm
MOE430A:1435414_S_AT dynactin 1 mm
MOUSE430A_2:1435414_S_AT dynactin 1 mm
MOUSE430_2:1435414_S_AT dynactin 1 mm
MOE430A:1422521_AT dynactin 1 mm
MOUSE430A_2:1422521_AT dynactin 1 mm
MOUSE430_2:1422521_AT dynactin 1 mm
MU19KSUBA:TC14507_AT Dynactin 1 (Dctn1), mRNA mm
MU19KSUBA:TC14507_G_AT Dynactin 1 (Dctn1), mRNA mm
RG-U34A:X62160_AT dynactin 1 rn
RAE230A:1367848_AT dynactin 1 rn
RAT230_2:1367848_AT dynactin 1 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5813 centrosome inferred from direct assay QuickGO AmiGO
5737 cytoplasm inferred from direct assay QuickGO AmiGO
5869 dynactin complex inferred from sequence similarity QuickGO AmiGO
776 kinetochore inferred from direct assay QuickGO AmiGO
30496 midbody inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8092 cytoskeletal protein binding inferred from sequence similarity QuickGO AmiGO
45505 dynein intermediate chain binding inferred from sequence similarity QuickGO AmiGO
8017 microtubule binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93427 Hypothetical protein ZK593.5 [Caenorhabditis elegans] ref|NP_502033.2| ZK593.5 [Caenorhabditis elegans] 0.0
blast CAE61960 Hypothetical protein CBG05960 [Caenorhabditis briggsae] 0.0
blast CAA44091 150K dynein-associated polypeptide [Rattus norvegicus] ref|NP_077044.1| dynactin 1 [Rattus norvegicus] sp|P28023|DYNA_RAT Dynactin-1 (150 kDa dynein-associated polypeptide) (DP-150) (DAP-150) (p150-glued) 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR000938 EMBL-EBI CAP-Gly domain 1.4E-30
Sequence
Target Sequence 
>C. ELEGANS:174591_AT
ctttgataaaggtgaatcgccgtcttcgtcgtgtatggtttgatgcgtggggagaagagt
atccattcgaaatcgcggccaaatctcgaccaatagtagcagtacctgaaccgaagaagt
tcgaagaaatcagtgccaagtggggaatcgtgtggtagactgccgaaattcatattaaat
catgacgtaacttctattagtatcataattctcgttttgcttcgctacgcgacgggttcg
cttgtaactttctcaaatatcatttttcaccattaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTTGATAAAGGTGAATCGCCGTCT 135 185 64 Antisense
GGAATCGTGTGGTAGACTGCCGAAA 257 531 207 Antisense
GTGGTAGACTGCCGAAATTCATATT 420 485 215 Antisense
AAATCATGACGTAACTTCTATTAGT 648 117 240 Antisense
GACGTAACTTCTATTAGTATCATAA 124 377 247 Antisense
AGTATCATAATTCTCGTTTTGCTTC 39 63 262 Antisense
CATAATTCTCGTTTTGCTTCGCTAC 672 213 267 Antisense
GCTACGCGACGGGTTCGCTTGTAAC 493 301 287 Antisense
GGGTTCGCTTGTAACTTTCTCAAAT 157 497 297 Antisense
CGCTTGTAACTTTCTCAAATATCAT 87 255 302 Antisense
TCAAATATCATTTTTCACCATTAAA 219 629 316 Antisense
Sequence Source Affymetrix Proprietary Database