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[174587_at]

Description CEK063H7R_rc /REP_DB=TREMBL Accession /GB=D71228 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk63h7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 54      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 82      6grp_total > 55      4grp_total > 57      6grp_cgc > 55      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 461
1 2 3 4 range
A 236.8
(-2)
P 		441
(1)
P 		582
(3)
P 		164.8
(-3)
P 		417
B 203.6
(-3)
P 		462.4
(1.4)
P 		555.8
(3)
P 		209
(-2.9)
P 		352
C 216.4
(-3)
P 		560.5
(2)
P 		625.7
(3)
P 		244.8
(-2.6)
P 		409
avr 218.9
(-2.8) 		488
(1.4) 		587.8
(3) 		206.2
(-3) 		382

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 237.1
(-3)
P 		417.8
(3)
P 		409.6
(2.7)
P 		333.5
(0.2)
P 		287
(-1.3)
P 		417
(3)
P 		181
B 313.3
(-0.1)
P 		404.6
(2.9)
P 		397.7
(2.7)
P 		339.5
(0.7)
P 		226.2
(-3)
P 		407.9
(3)
P 		182
C 248.4
(-3)
P 		378.4
(1.2)
P 		347.9
(0.3)
P 		282.6
(-1.9)
P 		263.3
(-2.5)
P 		432
(3)
P 		184
avr 266.3
(-2.7) 		400.3
(2.3) 		385.1
(1.7) 		318.5
(-0.8) 		258.8
(-3) 		419
(3) 		160
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 86.2
(-0.1)
P 		95.7
(0.4)
P 		152.1
(3)
P 		24.1
(-3)
P 		128
B 64.9
(-1.5)
P 		114.2
(2)
P 		128.8
(3)
P 		44.5
(-3)
P 		84
C 71.6
(-2)
P 		161.2
(1.1)
P 		218.1
(3)
P 		41.3
(-3)
P 		177
avr 74.2
(-1.3) 		123.7
(1) 		166.3
(3) 		36.6
(-3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 43.7
(-3)
P 		148.6
(3)
P 		89.2
(-0.4)
P 		84.6
(-0.7)
P 		86.6
(-0.5)
P 		101.5
(0.3)
P 		105
B 109
(-1.2)
P 		134.7
(1.3)
P 		96.6
(-2.4)
P 		89.9
(-3)
P 		91.9
(-2.8)
P 		152.1
(3)
P 		62
C 92.1
(-0.6)
P 		115.5
(1)
P 		88.6
(-0.8)
P 		56.8
(-3)
P 		85.2
(-1.1)
P 		144.6
(3)
P 		88
avr 81.6
(-2.5) 		132.9
(3) 		91.5
(-1.5) 		77.1
(-3) 		87.9
(-1.8) 		132.7
(3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174587_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21374
Sequence Type Exemplar sequence
Representative Public ID CEK063H7R NCBI
Target Description CEK063H7R_rc /REP_DB=TREMBL Accession /GB=D71228 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk63h7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070000(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070000 NCBI Caenorhabditis elegans HSN Abnormal Migration family member (ham-1) (ham-1) mRNA, complete cds. 11/11 None
F53B2.6 ENSEMBL cdna:known chromosome:CEL140:IV:12542005:12545685:1 gene:F53B2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12545343-12545636(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ham-1
Ensembl F53B2.6
Entrez Gene 178205 Entrez gene
SwissProt Q9XTH7 EMBL-EBI
WormBase CE17851 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98132 Hypothetical protein F53B2.6 [Caenorhabditis elegans] emb|CAB05844.1| Hypothetical protein F53B2.6 [Caenorhabditis elegans] ref|NP_502401.1| HSN Abnormal Migration family member (ham-1) [Caenorhabditis elegans] 0.0
blast CAE67925 Hypothetical protein CBG13523 [Caenorhabditis briggsae] 1.0E-171
blast XP_125685 PREDICTED: similar to winged helix domain-containing protein isoform A [Mus musculus] 9.0E-26
Sequence
Target Sequence 
>C. ELEGANS:174587_AT
aaatgtttagctatagttccacaaacacatcagaatccagtgaaattttgttttttttct
acttctatttttacccttacaaatagagtttgttagcgcacttttaagcccccacccatc
aaaacgtgtcttctttgttttcacccaatttcatactttttttgttacactttctgcgtc
tcttctactccatgttggagttgtgtctatgtctcttctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGTTTAGCTATAGTTCCACAAA 123 121 42 Antisense
GTTCCACAAACACATCAGAATCCAG 332 441 57 Antisense
CAGAATCCAGTGAAATTTTGTTTTT 122 205 72 Antisense
AATAGAGTTTGTTAGCGCACTTTTA 298 173 123 Antisense
GAGTTTGTTAGCGCACTTTTAAGCC 569 397 127 Antisense
CACCCATCAAAACGTGTCTTCTTTG 283 201 154 Antisense
TCTTCTTTGTTTTCACCCAATTTCA 255 617 170 Antisense
TGTTTTCACCCAATTTCATACTTTT 180 561 177 Antisense
CGTCTCTTCTACTCCATGTTGGAGT 27 247 218 Antisense
GTTGGAGTTGTGTCTATGTCTCTTC 218 437 235 Antisense
GGAGTTGTGTCTATGTCTCTTCTCC 549 517 238 Antisense
Sequence Source Affymetrix Proprietary Database