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[174581_s_at] acyl-CoA dehydrogenase

Description CEK060A1R_rc /REP_DB=TREMBL Accession /GB=D70965 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk60a1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 57      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 58      6grp_total > 59      4grp_total > 01      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2871
1 2 3 4 range
A 3627.4
(-1.5)
P 		3069.7
(-3)
P 		3847.2
(-0.9)
P 		5335.4
(3)
P 		2266
B 3170.2
(-3)
P 		3363.2
(-2.6)
P 		3387.7
(-2.5)
P 		5940.4
(3)
P 		2770
C 3409.9
(-2.7)
P 		3281.9
(-3)
P 		3371.1
(-2.8)
P 		5611.9
(3)
P 		2330
avr 3402.5
(-2.6) 		3238.3
(-3) 		3535.3
(-2.3) 		5629.2
(3) 		2391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2872
1 2 3 4 5 6 range
A 3838.3
(3)
P 		1407.5
(-3)
P 		2469.1
(-0.4)
P 		1945.1
(-1.7)
P 		2215.7
(-1)
P 		3713.2
(2.7)
P 		2431
B 4125.1
(2.6)
P 		1749.9
(-3)
P 		2891.8
(-0.3)
P 		2252
(-1.8)
P 		2329.9
(-1.6)
P 		4279.8
(3)
P 		2530
C 3319.3
(1.4)
P 		1847.1
(-3)
P 		2093.3
(-2.3)
P 		2057.9
(-2.4)
P 		2191.3
(-2)
P 		3839.6
(3)
P 		1993
avr 3760.9
(2.5) 		1668.2
(-3) 		2484.7
(-0.8) 		2085
(-1.9) 		2245.6
(-1.5) 		3944.2
(3) 		2276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 654
1 2 3 4 range
A 1320.7
(3)
P 		666.4
(-3)
P 		1005.7
(0.1)
P 		781
(-1.9)
P 		654
B 1010.9
(-0.2)
P 		830.6
(-2.4)
P 		785
(-3)
P 		1265.9
(3)
P 		481
C 1128.8
(1.8)
P 		943.6
(-3)
P 		1174.9
(3)
P 		946.3
(-2.9)
P 		231
avr 1153.5
(3) 		813.5
(-3) 		988.5
(0.1) 		997.7
(0.3) 		340

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1182
1 2 3 4 5 6 range
A 707.6
(0.1)
P 		500.6
(-2.9)
P 		537.7
(-2.4)
P 		493.3
(-3)
P 		668.6
(-0.4)
P 		904.1
(3)
P 		411
B 1435.3
(2)
P 		582.5
(-3)
P 		702.2
(-2.3)
P 		596.5
(-2.9)
P 		946.6
(-0.8)
P 		1595.9
(3)
P 		1013
C 1230.3
(2.6)
P 		563.6
(-2)
P 		532.8
(-2.2)
P 		413.8
(-3)
P 		709.1
(-1)
P 		1285.3
(3)
P 		872
avr 1124.4
(1.9) 		548.9
(-2.6) 		590.9
(-2.3) 		501.2
(-3) 		774.8
(-0.8) 		1261.8
(3) 		761
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 174581_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22377
Sequence Type Exemplar sequence
Representative Public ID CEK060A1R NCBI
Target Description CEK060A1R_rc /REP_DB=TREMBL Accession /GB=D70965 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk60a1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078388(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078388 NCBI Caenorhabditis elegans T25G12.5 (T25G12.5) mRNA, complete cds. 11/11 A 
SNAP00000027868 ENSEMBL cdna:SNAP chromosome:CEL140:X:17215032:17216957:-1 11/11 None
GENEFINDER00000027885 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17215032:17217564:-1 11/11 None
T25G12.5.1 ENSEMBL cdna:known chromosome:CEL140:X:17214930:17217019:-1 gene:T25G12.5 11/11 None
T25G12.5.2 ENSEMBL cdna:known chromosome:CEL140:X:17214941:17217017:-1 gene:T25G12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17214955-17215297(-) 98.84 98.84
Public Domain and Genome References
Gene Title acyl-CoA dehydrogenase
Ensembl T25G12.5
Entrez Gene 181758 Entrez gene
SwissProt Q22781 EMBL-EBI
WormBase CE18284 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3995 acyl-CoA dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69025 Hypothetical protein T25G12.5 [Caenorhabditis elegans] ref|NP_510789.1| T25G12.5 [Caenorhabditis elegans] 0.0
blast CAE69690 Hypothetical protein CBG15945 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-152
ec ACDM_DROME (Q9VSA3) Probable acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-157
ec ACDM_DROME (Q9VSA3) Probable acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-162
Protein Domains
Database ID Description E-Value
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 2.6E-70
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 6.1E-61
Pfam IPR006092 EMBL-EBI Acyl-CoA dehydrogenase, N-terminal 1.3E-24
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 2.6E-70
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 6.1E-61
Pfam IPR006092 EMBL-EBI Acyl-CoA dehydrogenase, N-terminal 1.3E-24
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 2.6E-70
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 6.1E-61
Pfam IPR006092 EMBL-EBI Acyl-CoA dehydrogenase, N-terminal 1.3E-24
Sequence
Target Sequence 
>C. ELEGANS:174581_S_AT
caaatctttggaggagctggtttcaacactgaatacccagcggagaagctgatgcgtgac
gccaagatcttccaaatctacgagggaacttcgcaaattcaacgcatggtcattgctcgt
cagttgctcgctcgagtcgctcagaatggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAATCTTTGGAGGAGCTGGTTTCA 670 191 120 Antisense
GGAGGAGCTGGTTTCAACACTGAAT 578 515 129 Antisense
GGTTTCAACACTGAATACCCAGCGG 243 505 138 Antisense
ACACTGAATACCCAGCGGAGAAGCT 677 107 145 Antisense
CGGAGAAGCTGATGCGTGACGCCAA 580 243 160 Antisense
TGATGCGTGACGCCAAGATCTTCCA 317 565 169 Antisense
GACGCCAAGATCTTCCAAATCTACG 605 381 177 Antisense
GAGGGAACTTCGCAAATTCAACGCA 396 335 201 Antisense
ATTCAACGCATGGTCATTGCTCGTC 689 11 216 Antisense
GCATGGTCATTGCTCGTCAGTTGCT 555 309 223 Antisense
CTCGCTCGAGTCGCTCAGAATGGAG 164 233 246 Antisense
Sequence Source Affymetrix Proprietary Database