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[174575_at] YK10 homolog

Description CEC2533_rc /REP_DB=TREMBL Accession /GB=C10253 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk125e8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 20      4grp_high_8_8 > 39      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 123.7
(-1.2)
P 		105.7
(-2.8)
P 		170.9
(3)
P 		103.8
(-3)
P 		67
B 85.5
(-3)
P 		97
(-1.6)
P 		118.2
(0.9)
P 		135.3
(3)
P 		50
C 116.6
(-3)
P 		124.3
(3)
P 		122.4
(1.5)
P 		120.6
(0.1)
P 		8
avr 108.6
(-3) 		109
(-2.9) 		137.2
(3) 		119.9
(-0.6) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 94.9
(3)
P 		92.6
(2.2)
P 		76.9
(-3)
P 		87.7
(0.6)
P 		83.6
(-0.8)
P 		91.4
(1.8)
P 		18
B 198.8
(3)
P 		143.1
(0.3)
P 		81.5
(-2.7)
P 		115.5
(-1)
P 		74.8
(-3)
P 		85.7
(-2.5)
P 		124
C 151.2
(3)
P 		149.8
(2.9)
P 		70.8
(-3)
P 		141.2
(2.3)
P 		113.5
(0.2)
P 		73.4
(-2.8)
P 		80
avr 148.3
(3) 		128.5
(1.3) 		76.4
(-3) 		114.8
(0.2) 		90.6
(-1.8) 		83.5
(-2.4) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 45
(3)
P 		23
(-1.4)
P 		44.7
(2.9)
P 		15.2
(-3)
P 		30
B 27.3
(1.2)
P 		23.9
(-3)
P 		27.4
(1.3)
P 		28.8
(3)
P 		5
C 38.6
(1.9)
P 		35.7
(1.1)
P 		42.7
(3)
P 		20.3
(-3)
P 		22
avr 37
(2.5) 		27.5
(-0.8) 		38.3
(3) 		21.4
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 17.5
(-2.7)
P 		32.9
(3)
P 		16.7
(-3)
P 		22.2
(-1)
P 		25.2
(0.1)
P 		22.3
(-0.9)
P 		16
B 69.2
(3)
P 		47.6
(0.4)
P 		19.8
(-3)
P 		30.6
(-1.7)
P 		30.4
(-1.7)
P 		32
(-1.5)
P 		49
C 56
(3)
P 		45.7
(1.4)
P 		18
(-3)
P 		28.4
(-1.4)
P 		36.7
(-0)
P 		24.6
(-2)
P 		38
avr 47.6
(3) 		42.1
(1.9) 		18.2
(-3) 		27.1
(-1.2) 		30.8
(-0.4) 		26.3
(-1.3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174575_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20906
Sequence Type Exemplar sequence
Representative Public ID CEC2533 NCBI
Target Description CEC2533_rc /REP_DB=TREMBL Accession /GB=C10253 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk125e8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066878(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066878 NCBI Caenorhabditis elegans T07A5.2 (T07A5.2) mRNA, complete cds. 11/11 None
T07A5.2 ENSEMBL cdna:known chromosome:CEL140:III:10314472:10318039:-1 gene:T07A5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10314477-10314768(-) 100.0 100.0
Public Domain and Genome References
Gene Title YK10 homolog
Ensembl T07A5.2
Entrez Gene 176443 Entrez gene
SwissProt Q10045 EMBL-EBI
WormBase CE36501 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13583_AT UNC-50 family protein at
ATH1-121501:263303_AT UNC-50 family protein at
CANINE:1591503_AT similar to unc-50 homolog cfa
CANINE_2:CFA.10690.1.S1_AT similar to unc-50 homolog cfa
CANINE_2:CFA.10690.1.S1_S_AT similar to unc-50 homolog cfa
DROSOPHILA_2:1635637_AT dm
DROSGENOME1:149516_AT dm
CHICKEN:GGA.12244.1.S1_AT unc-50 homolog (C. elegans) gga
HG-U133A_2:203583_AT unc-50 homolog (C. elegans) hs
HG-U133A:203583_AT unc-50 homolog (C. elegans) hs
HG-U133_PLUS_2:203583_AT unc-50 homolog (C. elegans) hs
U133_X3P:G7661609_3P_AT unc-50 homolog (C. elegans) hs
HG-U95AV2:39442_AT unc-50 homolog (C. elegans) hs
HG-U133_PLUS_2:239505_AT Unc-50 homolog (C. elegans) hs
HG-U133B:239505_AT Unc-50 homolog (C. elegans) hs
HG-U95D:76392_AT Unc-50 homolog (C. elegans) hs
U133_X3P:HS.121740.0.S1_3P_AT Unc-50 homolog (C. elegans) hs
MOUSE430_2:1421982_A_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1421982_A_AT unc-50 homolog (C. elegans) mm
MOE430A:1421982_A_AT unc-50 homolog (C. elegans) mm
MG-U74AV2:96675_AT unc-50 homolog (C. elegans) mm
MOE430A:1435812_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1435812_AT unc-50 homolog (C. elegans) mm
MOUSE430_2:1435812_AT unc-50 homolog (C. elegans) mm
MOE430A:1435811_A_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1435811_A_AT unc-50 homolog (C. elegans) mm
MOUSE430_2:1435811_A_AT unc-50 homolog (C. elegans) mm
MU19KSUBB:TC27432_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBB:TC27432_G_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBB:TC27433_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBC:TC38061_S_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBC:TC38062_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
RG-U34A:RC_AI008423_AT unc-50 homolog (C. elegans) rn
RAE230A:1398865_AT unc-50 homolog (C. elegans) rn
RAT230_2:1398865_AT unc-50 homolog (C. elegans) rn
YEAST_2:1776281_AT Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Sc
Protein Similarities
Method ID Description E-Value
blast CAA88132 Hypothetical protein T07A5.2 [Caenorhabditis elegans] ref|NP_499279.2| T07A5.2 [Caenorhabditis elegans] sp|Q10045|YRT2_CAEEL Hypothetical protein T07A5.2 in chromosome III 1.0E-165
blast CAE56800 Hypothetical protein CBG24612 [Caenorhabditis briggsae] 1.0E-153
blast XP_785090 PREDICTED: similar to unc-50 homolog [Strongylocentrotus purpuratus] 8.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007881 EMBL-EBI UNC-50 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499279.2 5 116-138,148-170,191-213,228-250,255-277
T07A5.2 5 116-138,148-170,191-213,228-250,255-277
Sequence
Target Sequence 
>C. ELEGANS:174575_AT
tgtccctctctaatttgtttgtaaattttttagtgatttttgagctatctggaagtgaaa
atatggtttaaaatgggtctcgtagcgattttctcacaactttggagccatgcgttggcg
gtaattaatttcccttcaagacccccactccaaattttcatctccagcaatataaaaatt
gcttcgaaaatagttaattttttcaactatttatactgcatgatggtgtgctgaaaacct
aaccgattttccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCCCTCTCTAATTTGTTTGTAAA 359 557 18 Antisense
GTGATTTTTGAGCTATCTGGAAGTG 702 483 50 Antisense
TATGGTTTAAAATGGGTCTCGTAGC 669 607 79 Antisense
GTCTCGTAGCGATTTTCTCACAACT 299 471 94 Antisense
GTAGCGATTTTCTCACAACTTTGGA 562 455 99 Antisense
TTCTCACAACTTTGGAGCCATGCGT 637 691 108 Antisense
TTTGGAGCCATGCGTTGGCGGTAAT 555 665 118 Antisense
TGGCGGTAATTAATTTCCCTTCAAG 354 543 133 Antisense
AACTATTTATACTGCATGATGGTGT 576 137 222 Antisense
TGGTGTGCTGAAAACCTAACCGATT 85 551 241 Antisense
GCTGAAAACCTAACCGATTTTCCAT 500 289 247 Antisense
Sequence Source Affymetrix Proprietary Database