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[174574_at]

Description CEK006C5R_rc /REP_DB=TREMBL Accession /GB=D27938 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk6c5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 67      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 92      6grp_total > 67      4grp_total > 12      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 592
1 2 3 4 range
A 539.3
(0.1)
P 		361.7
(-1.9)
P 		258.8
(-3)
P 		808.1
(3)
P 		549
B 456.5
(-1)
P 		398
(-1.6)
P 		271.6
(-3)
P 		831.2
(3)
P 		560
C 407.5
(-1.8)
P 		294.8
(-3)
P 		346.7
(-2.4)
P 		851
(3)
P 		556
avr 467.8
(-1) 		351.5
(-2.3) 		292.4
(-3) 		830.1
(3) 		538

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 445
1 2 3 4 5 6 range
A 291.2
(-2.6)
P 		291.9
(-2.6)
P 		420.3
(-0.6)
P 		300.7
(-2.4)
P 		263.2
(-3)
P 		657.4
(3)
P 		394
B 412.7
(-0.3)
P 		267.5
(-2.6)
P 		246.6
(-2.9)
P 		243.1
(-3)
P 		261
(-2.7)
P 		625.1
(3)
P 		382
C 344.3
(-1.1)
P 		266.6
(-2.2)
P 		355
(-1)
P 		212.1
(-3)
P 		360.1
(-0.9)
P 		632.2
(3)
P 		420
avr 349.4
(-1.5) 		275.3
(-2.6) 		340.6
(-1.6) 		252
(-3) 		294.8
(-2.3) 		638.2
(3) 		386
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 196.4
(3)
P 		78.5
(-2.5)
P 		67.7
(-3)
P 		118.3
(-0.6)
P 		129
B 145.6
(1.3)
P 		98.3
(-1.1)
P 		62.9
(-3)
P 		177.1
(3)
P 		114
C 134.9
(2.1)
P 		84.8
(-3)
P 		120.8
(0.7)
P 		143.5
(3)
P 		59
avr 159
(3) 		87.2
(-2.7) 		83.8
(-3) 		146.3
(2) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 53.7
(-3)
P 		103.8
(-0.2)
P 		91.5
(-0.9)
P 		76.3
(-1.7)
P 		79.4
(-1.6)
P 		160.1
(3)
P 		106
B 143.6
(-0.1)
P 		89
(-2)
P 		59.9
(-3)
P 		64.4
(-2.8)
P 		106.1
(-1.4)
P 		233.1
(3)
P 		173
C 127.6
(0)
P 		81.3
(-1.6)
P 		90.4
(-1.3)
P 		42.6
(-3)
P 		116.5
(-0.4)
P 		211.6
(3)
P 		169
avr 108.3
(-1) 		91.4
(-1.7) 		80.6
(-2.2) 		61.1
(-3) 		100.7
(-1.3) 		201.6
(3) 		141
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174574_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20634
Sequence Type Exemplar sequence
Representative Public ID CEK006C5R NCBI
Target Description CEK006C5R_rc /REP_DB=TREMBL Accession /GB=D27938 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk6c5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027085(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027085 NCBI Caenorhabditis elegans M106.4a (M106.4) mRNA, complete cds. 11/11 None
M106.4a ENSEMBL cdna:known chromosome:CEL140:II:10863604:10868719:-1 gene:M106.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174672 2 1 NM_001027085 174574_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10863611-10863939(-) 93.6 100.0
Public Domain and Genome References
Ensembl M106.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA87052 Hypothetical protein M106.4a [Caenorhabditis elegans] ref|NP_001022256.1| M106.4a [Caenorhabditis elegans] sp|Q09580|GUAA_CAEEL Probable GMP synthase [glutamine-hydrolyzing] (Glutamine amidotransferase) 0.0
blast CAE48508 Hypothetical protein M106.4b [Caenorhabditis elegans] ref|NP_001022257.1| M106.4b [Caenorhabditis elegans] 0.0
blast CAE59594 Hypothetical protein CBG03001 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000991 EMBL-EBI Glutamine amidotransferase class-I 4.0E-45
Pfam IPR001674 EMBL-EBI GMP synthase, C-terminal 2.3E-114
Sequence
Target Sequence 
>C. ELEGANS:174574_AT
tttatatcattttcatctcttaaaagaaaggtttttcaggtaattgttaaatagattatt
gttgtatcatgttgattttgattcttcgttttttctcatcccgggtgcacttttccaacg
tcctctgttccctccccaatttttttgctccgattttatgtgttttaaagcgttaaacgt
cagttcccagtgccgagttttcaaaaggtgtccatcggttttttttttaaattttttatc
tagtgctcaacgataccgatgaacatttccgagcttggcgtgaatcccgatagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTATATCATTTTCATCTCTTAAAA 129 675 16 Antisense
TTATTGTTGTATCATGTTGATTTTG 347 679 71 Antisense
TGTTGATTTTGATTCTTCGTTTTTT 579 563 85 Antisense
AAGCGTTAAACGTCAGTTCCCAGTG 298 147 183 Antisense
TTAAATTTTTTATCTAGTGCTCAAC 31 683 242 Antisense
TTTTATCTAGTGCTCAACGATACCG 7 673 249 Antisense
GCTCAACGATACCGATGAACATTTC 600 301 260 Antisense
GATACCGATGAACATTTCCGAGCTT 518 423 267 Antisense
GATGAACATTTCCGAGCTTGGCGTG 207 413 273 Antisense
TGAACATTTCCGAGCTTGGCGTGAA 597 573 275 Antisense
GAGCTTGGCGTGAATCCCGATAGAT 674 387 286 Antisense
Sequence Source Affymetrix Proprietary Database